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US4468470A
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Method and a kit for the assay of antibodies to soluble antigens
Assignee: STICHTING CENTRAAL LABORATORIUM VAN DE BLOEDTRANSFUSIE-DIEST VAN HET NEDERLANDSE RODE KRUIS
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Filing: 1982-04-30
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Granted: 1984-08-28
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Expires: 2002-04-30
Abstract
Abstract The invention relates to a method for the assay of antibodies to soluble antigens in an aqueous sample, in particular in body fluids, such as blood serum or blood plasma, by contacting said sample with an antigen in vitro, wherein antibodies, if present, are bound by said antigens. The invention furthermore relates to a kit for the assay and detection of antigen-specific antibodies. According to the invention an antigen is used that is modified with a recognizable group, and said modified antigen is soluble in the test sample. The interaction between antigen and antibody takes place in homogeneous solution.
Inventors
- 👤 Robertus C. Aalberse
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 10:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/538; Y; Y10; Y10S; Y10S436/00; Y10S436/815
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
116 words
In a method for the assay of antibodies to soluble antigens in an aqueous sample, by contacting said sample with antigens in vitro, wherein antibodies, if present, are bound by said antigens, the improvement which comprises utilizing an antigen that is modified with a recognizable group, said modified antigen being soluble in the test sample so that an interaction between antigen and antibody occurs in a homogeneous solution, and reacting the modified antigen with the antibody bound to it, if present, with a precipitating or an insoluble reagent that interacts with the recognizable group, wherein said bound antibody is separated from the sample solution while unbound antibody remains in solution, and analyzing said bound separated antibody.
Claim 2
Dependent Claim
116 words
In a method for the assay of antibodies to soluble antigens in an aqueous sample, by contacting said sample with antigens in vitro, wherein antibodies, if present, are bound by said antigens, the improvement which comprises utilizing an antigen that is modified with a recognizable group, said modified antigen being soluble in the test sample so that an interaction between antigen and antibody occurs in a homogeneous solution, and reacting the modified antigen with the antibody bound to it, if present, with a precipitating or an insoluble reagent that interacts with the recognizable group, wherein said bound antibody is separated from the sample solution while unbound antibody remains in solution, and analyzing said bound separated antibody.
Claim 3
Dependent Claim
25 words
The method according to claim 1, characterized in that hapten is used as recognizable group and anti-hapten antibody coupled to a solid-phase as insoluble reagent.
Claim 4
Dependent Claim
14 words
The method according to claim 2, characterized in that as hapten, trinitrophenyl is used.
Claim 5
Dependent Claim
25 words
The method according to claim 1, characterized in that as recognizable group biotin is used and as insoluble reagent avidin, coupled to a solid-phase carrier.
Claim 6
Independent Claim
19 words
The method according to claims 2, 3, or 4, characterized in that as a solid-phase plastic beads are used.
Claim 7
Independent Claim
21 words
The method according to claims 2, 3, or 4, characterized in that as a solid-phase carrier a microtiter plate is used
Claim 8
Dependent Claim
18 words
The method according to claim 2 characterized in that as solid-phase carrier chemically activated insoluble polymers are used.
Claim 9
Dependent Claim
17 words
The method according to claim 7, characterized in that as insoluble polymers CNBr-activated agarose beads are used.
Claim 10
Dependent Claim
85 words
A kit for the assay and determination of antibodies directed against certain antigens in a homogeneous aqueous solution characterized in that said kit contains a first reagent consisting of an aqueous solution including a soluble antigen that is modified with a recognizable group, said modified antigen being reactable with the antibodies to be determined, a second reagent consisting of a precipitating or insoluble reagent that can interact with the recognizable group, and a third reagent consisting of labelled anti-Ig combinable with antibodies to be determined.
Claim 11
Dependent Claim
85 words
A kit for the assay and determination of antibodies directed against certain antigens in a homogeneous aqueous solution characterized in that said kit contains a first reagent consisting of an aqueous solution including a soluble antigen that is modified with a recognizable group, said modified antigen being reactable with the antibodies to be determined, a second reagent consisting of a precipitating or insoluble reagent that can interact with the recognizable group, and a third reagent consisting of labelled anti-Ig combinable with antibodies to be determined.
Claim 12
Dependent Claim
17 words
The kit according to claim 9, characterized in that one or more of the reagents are lyophilized.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-28 | 1988-02-27 | 1988-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-28 | 1992-02-27 | 1992-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-28 | 1996-02-27 | 1996-08-28 | $7700.00 | Window Closed / Lapsed |