US4021534A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Radioimmunoassay
Assignee: Hoffmann La Roche Inc
•
Filing: 1975-12-12
•
Granted: 1977-05-03
•
Expires: 1995-12-12
Abstract
Abstract Soluble antibody-antigen complexes with radiolabeled antigen are made insoluble and separated from soluble unreacted radiolabeled antigen by means of an insoluble complex of para-amino benzyl cellulose and an antibody to the antibody in the antibody-antigen complex.
Inventors
- 👤 George Stanford Lafontaine
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 09:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/813
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
36 words
A method of insolubilizing soluble antigen-antibody complexes which comprises reacting said antigen-antibody complex in solution with a second antibody directed against the antibody in the soluble antigen-antibody complex said second antibody being bound to para-aminobenzyl cellulose.
Claim 2
Independent Claim
36 words
A method of insolubilizing soluble antigen-antibody complexes which comprises reacting said antigen-antibody complex in solution with a second antibody directed against the antibody in the soluble antigen-antibody complex said second antibody being bound to para-aminobenzyl cellulose.
Claim 3
Independent Claim
53 words
A method of separating soluble antigen-antibody complexes from soluble antigen contained in said complex which comprises insolubilizing said antigen-antibody complex by reacting said antigen-antibody complex in solution with a second antibody directed against the antibody in the soluble antigen-antibody complex, said second antibody being bound to para-aminobenzyl cellulose, and separating the insolubilized complex.
Claim 4
Independent Claim
53 words
A method of separating soluble antigen-antibody complexes from soluble antigen contained in said complex which comprises insolubilizing said antigen-antibody complex by reacting said antigen-antibody complex in solution with a second antibody directed against the antibody in the soluble antigen-antibody complex, said second antibody being bound to para-aminobenzyl cellulose, and separating the insolubilized complex.
Claim 5
Dependent Claim
50 words
In an indirect radioimmunoassay procedure, wherein a radiolabeled antigen complexed with its antibody is separated from unreacted radiolabeled antigen, the improvement which comprises insolubilizing the antigen-antibody complex by reacting it with a second antibody directed against the antibody in the antigen-antibody complex, said second antibody being bound to para-aminobenzyl cellulose.
Claim 6
Dependent Claim
50 words
In an indirect radioimmunoassay procedure, wherein a radiolabeled antigen complexed with its antibody is separated from unreacted radiolabeled antigen, the improvement which comprises insolubilizing the antigen-antibody complex by reacting it with a second antibody directed against the antibody in the antigen-antibody complex, said second antibody being bound to para-aminobenzyl cellulose.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the antigen is carcinoembryonic antigen.
Claim 8
Dependent Claim
15 words
The method of claim 3 wherein the radiolabeled antigen is carcinoembryonic antigen labeled with iodine-125.
Claim 9
Dependent Claim
11 words
The method of claim 3 wherein the second antibody is anti-IgG.
Claim 10
Dependent Claim
5 words
Anti-IgG bound to para-aminobenzyl cellulose.
Claim 11
Dependent Claim
5 words
Anti-IgG bound to para-aminobenzyl cellulose.
Claim 12
Dependent Claim
26 words
An antigen-antibody complex consisting essentially of as the antibody anti-IgG bound to para-aminobenzyl cellulose and as the antigen the complex of carcinoembryonic antigen and its antibody.
Claim 13
Dependent Claim
26 words
An antigen-antibody complex consisting essentially of as the antibody anti-IgG bound to para-aminobenzyl cellulose and as the antigen the complex of carcinoembryonic antigen and its antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-02 | 1980-11-01 | 1981-05-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-02 | 1984-11-01 | 1985-05-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-02 | 1988-11-01 | 1989-05-03 | $7700.00 | Window Closed / Lapsed |