US4447529A
Expired / Lapsed
US
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Preparing homogeneous specific binding assay element to avoid premature reaction
Assignee: Bayer Corp
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Filing: 1981-07-06
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Granted: 1984-05-08
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Expires: 2001-07-06
Abstract
Abstract A homogeneous specific binding assay element, a method for its preparation, and a method for its use in determining a ligand in or the ligand binding capacity of a liquid sample. The test element comprises a solid carrier incorporated with reagents for a homogeneous specific binding assay system which produces a detectable response, usually an electromagnetic radiation signal, that is a function of the presence or amount of the ligand in or the ligand binding capacity of the sample. Also a method for preparing a homogeneous specific binding assay element for determining a ligand in or the ligand binding capacity of a liquid sample by incorporating a carrier with a composition which includes a label conjugate, comprising a label component coupled to a ligand moiety or a specific binding analog thereof, and a reagent reactive with the label conjugate, which method comprises (a) incorporating the carrier with the reagent reactive with the label conjugate in a first liquid and drying the carrier; and then (b) incorporating the carrier of (a) with the label conjugate in a liquid effective to prevent reaction with the reagent reactive with the label conjugate prior to contact of the element with the sample and drying the carrier.
Inventors
- 👤 Alfred C. Greenquist
- 👤 Patricia A. Rupchock
- 👤 Richard J. Tyhach
- 👤 Bert Walter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/968; Y10S435/971; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
130 words
A method for preparing a test device useful in performing a homogeneous specific binding assay to determine the presence of a ligand in, or the ligand binding capacity of, a liquid test sample, the method comprising the steps of incorporating a carrier with a first reagent mixture comprising water, a reagent capable of reacting with a label to produce a detectable response, and a specific binding partner to the ligand; drying the carrier incorporated with the first reagent mixture; incorporating the carrier with a second reagent mixture comprising a solvent selected from toluene, acetone, chloroform, methylene chloride, n-propanol and ethylene dichloride, and a label conjugate comprising the label coupled to a ligand moiety or a specific binding analogue thereof; and drying the carrier incorporated with the first and second mixtures.
Claim 2
Independent Claim
130 words
A method for preparing a test device useful in performing a homogeneous specific binding assay to determine the presence of a ligand in, or the ligand binding capacity of, a liquid test sample, the method comprising the steps of incorporating a carrier with a first reagent mixture comprising water, a reagent capable of reacting with a label to produce a detectable response, and a specific binding partner to the ligand; drying the carrier incorporated with the first reagent mixture; incorporating the carrier with a second reagent mixture comprising a solvent selected from toluene, acetone, chloroform, methylene chloride, n-propanol and ethylene dichloride, and a label conjugate comprising the label coupled to a ligand moiety or a specific binding analogue thereof; and drying the carrier incorporated with the first and second mixtures.
Claim 3
Dependent Claim
25 words
The method of claim 1 wherein the reagent of the first reagent mixture comprises a component capable of cleaving the label from the label conjugate.
Claim 4
Dependent Claim
11 words
The method of claim 1 wherein the label is a fluor.
Claim 5
Dependent Claim
10 words
The method of claim 1 wherein the label is umbelliferone.
Claim 6
Dependent Claim
30 words
The method of claim 1 in which the reagent of the first reagent mixture comprises β-galactosidase and antibody to the ligand, the label is β-galactosyl-umbelliferone, and the solvent is acetone.
Claim 7
Dependent Claim
37 words
The method of claim 1 in which the ligand is an aminoglycoside antibiotic, the reagent of the first reagent mixture comprises β-galactosidase and antibody to the aminoglycoside antibiotic, the label is β-galactosyl-umbelliferone, and the solvent is acetone.
Claim 8
Dependent Claim
33 words
The method of claim 1 in which the ligand is gentamycin, the reagent of the first reagent mixture comprises β-galactosidase and antibody to gentamycin, the label is β-galactosyl-umbelliferone, and the solvent is acetone.
Claim 9
Dependent Claim
33 words
The method of claim 1 in which the ligand is tobramycin, the reagent of the first reagent mixture comprises β-galactosidase and antibody to tobramycin, the label is β-galactosyl-umbelliferone and the solvent is acetone.
Claim 10
Dependent Claim
18 words
The method of claim 1 wherein the label of the label conjugate is a substrate for an enzyme.
Claim 11
Dependent Claim
17 words
The method of claim 9 wherein the substrate is a β-galactoside moiety and the enzyme is β-galactosidase.
Claim 12
Dependent Claim
10 words
The method of claim 10 wherein the label is β-galactosyl-umbelliferone.
Claim 13
Dependent Claim
67 words
The method of claim 1 which further comprises, prior to incorporating the carrier with the first reagent mixture, the steps of incorporating the carrier with an indicator mixture comprising an indicator reactive with the reagent of the first reagent mixture to produce a detectable response, and a solvent selected from toluene acetone, chloroform, methylene chloride, n-propanol and ethylene dichloride; and drying the carrier incorporated with the indicator.
Claim 14
Dependent Claim
23 words
The method of claim 12 wherein the solvent is the same as that used to incorporate the carrier with the second reagent mixture.
Claim 15
Dependent Claim
11 words
The method of claim 12 wherein the indicator reagent comprises 3,3',5,5'-tetramethylbenzidine.
Claim 16
Dependent Claim
31 words
The method of claim 12 wherein the reagent of the first reagent mixture comprises glucose, glucose oxidase apoenzyme, peroxidase and antibody to the ligand, and the label comprises flavine adenine dinucleotide.
Claim 17
Dependent Claim
21 words
The method of claim 15 wherein the reagent of the first reagent mixture further comprises bovine serum albumin and polyvinyl alcohol.
Claim 18
Dependent Claim
29 words
An analytical element for determining a ligand in or the ligand binding capacity of a liquid sample, which element is prepared by the method of any of claims 1-8.
Claim 19
Independent Claim
40 words
A method for determining the presence of a ligand in or the ligand binding capacity of a test sample, the method comprising contacting the test sample with a test device prepared by the method of any one of claims 1-8.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-08 | 1987-11-07 | 1988-05-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-08 | 1991-11-07 | 1992-05-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-08 | 1995-11-07 | 1996-05-08 | $7700.00 | Window Closed / Lapsed |