Patent US4447526A successfully refreshed from Google Patents.
US4447526A
Expired / Lapsed
US
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Homogeneous specific binding assay with carrier matrix incorporating specific binding partner
Assignee: Bayer Corp
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Filing: 1981-04-20
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Granted: 1984-05-08
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Expires: 2001-04-20
Abstract
Abstract A method for determining the presence of a ligand in, or the ligand binding capacity of a liquid test sample which includes the steps of (a) adding to the sample a conjugate of the ligand and a label, (b) contacting the sample with a test device containing reagents which in conjunction with the conjugate and ligand, are capable of producing a detectable response, and (c) measuring the response.
Inventors
- 👤 Patricia A. Rupchock
- 👤 Richard J. Tyhach
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:49
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/971; Y10S436/00; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
146 words
A method for determining the presence of a ligand in, or the ligand binding capacity of a liquid test sample, the method comprising the steps of forming a solution or suspension of a labeled conjugate and the liquid sample, the conjugate comprising the ligand or a specific binding analog of the ligand and a label, whereby a characteristic of the label is different when the conjugate is bound by a specific binding partner for said ligand or analog, compared to when said conjugate is not so bound, said difference in said characteristic being detectable, contacting said sample with a carrier matrix incorporated with reagents which, when in the presence of said conjugate, produce a homogeneous specific binding assay system wherein said difference in said label characteristic is detectable, said reagents comprising said specific binding partner, thereby producing said difference in said characteristic, and measuring said difference.
Claim 2
Independent Claim
146 words
A method for determining the presence of a ligand in, or the ligand binding capacity of a liquid test sample, the method comprising the steps of forming a solution or suspension of a labeled conjugate and the liquid sample, the conjugate comprising the ligand or a specific binding analog of the ligand and a label, whereby a characteristic of the label is different when the conjugate is bound by a specific binding partner for said ligand or analog, compared to when said conjugate is not so bound, said difference in said characteristic being detectable, contacting said sample with a carrier matrix incorporated with reagents which, when in the presence of said conjugate, produce a homogeneous specific binding assay system wherein said difference in said label characteristic is detectable, said reagents comprising said specific binding partner, thereby producing said difference in said characteristic, and measuring said difference.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein said difference is measured by human observation.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein said difference is measured by instrumental means.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein said carrier matrix comprises a substance absorbent to said liquid sample.
Claim 6
Dependent Claim
11 words
The method of claim 1 wherein said carrier matrix is paper.
Claim 7
Dependent Claim
15 words
The method of claim 1 wherein said carrier matrix comprises a polymeric film or gel.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein said label participates in an enzymatic reaction.
Claim 9
Dependent Claim
14 words
The method of claim 7 wherein said label is a substrate for an enzyme.
Claim 10
Dependent Claim
21 words
The method of claim 8 wherein said enzyme is capable of acting on said substrate label to produce a detectable product.
Claim 11
Dependent Claim
11 words
The method of claim 9 wherein said detectable product is fluorescent.
Claim 12
Dependent Claim
28 words
The method of claim 7 wherein said label is a prosthetic group of an enzyme, said groups being capable of combining with an apoenzyme to form said enzyme.
Claim 13
Dependent Claim
18 words
The method of claim 11 wherein said label comprises flavin adenine dinucleotide and said apoenzyme is apoglucose oxidase.
Claim 14
Dependent Claim
16 words
The method of claim 1 wherein difference is the appearance of or a change in color.
Claim 15
Dependent Claim
17 words
The method of claim 1 wherein said difference is the appearance of or a change in chemiluminescence.
Claim 16
Dependent Claim
17 words
The method of claim 1 wherein said difference is the appearance of or a change in fluorescence.
Claim 17
Dependent Claim
25 words
The method of claim 1 in which said difference is the appearance of or change in fluorescence, chemiluminescence, phosphorescence, light absorption, light reflectance or color.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-08 | 1987-11-07 | 1988-05-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-08 | 1991-11-07 | 1992-05-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-08 | 1995-11-07 | 1996-05-08 | $7700.00 | Window Closed / Lapsed |