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US4446316A
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Dextran derivative of fibrinolysin
Assignee: Vsesojuzny Kardiologichesky Nauchny Tsentr
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Filing: 1981-12-09
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Granted: 1984-05-01
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Expires: 2001-12-09
Abstract
Abstract A method of preparing a polysaccharide derivative of fibrinolysin which comprises reacting fibrinolysin with dextran dialdehyde having a molecular mass of from 20,000 to 110,000 at a pH of from 8.0 to 9.5 and at a temperature within the range of from 5 DEG to 30 DEG C. with the formation of a covalent bond; the resulting fibrinolysin derivative is reduced with sodium borohydride and isolated.
Inventors
- 👤 Evgeny I. Chazov
- 👤 Vladimir N. Smirnov
- 👤 Vladimir P. Torchilin
- 👤 Igor M. Tereshin
- 👤 Boris V. Moskvichev
- 👤 Grigory M. Grinberg
- 👤 Agris Z. Skuya
- 👤 Gersh I. Kleiner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:26
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6435; C08; C08B; C08B37/00; C12N9/96; C12Y; C12Y304/00; C12Y304/21; C12Y304/21007; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
66 words
A method of preparing a polysaccharide derivative of fibrinolysin having thrombolytic activity comprising reacting fibrinolysin with dextran dialdehyde having a molecular mass of from 20,000 to 110,000 at a pH of 8.0 to 9.5 and at a temperature within the range of from 5° to 30° C. to form a covalent bond, reducing the resulting polysaccharide derivative of fibrinolysin with sodium borohydride and isolating the product.
Claim 2
Independent Claim
66 words
A method of preparing a polysaccharide derivative of fibrinolysin having thrombolytic activity comprising reacting fibrinolysin with dextran dialdehyde having a molecular mass of from 20,000 to 110,000 at a pH of 8.0 to 9.5 and at a temperature within the range of from 5° to 30° C. to form a covalent bond, reducing the resulting polysaccharide derivative of fibrinolysin with sodium borohydride and isolating the product.
Claim 3
Dependent Claim
24 words
A method as claimed in claim 1, wherein sodium borohydride is used in a ratio to the polysaccharide derivative of fibrinolysin equal to 1.5-2.0:1.
Claim 4
Dependent Claim
66 words
A polysaccharide derivative of fibrinolysin having thrombolytic activity obtained by the steps comprising reacting fibrinolysin with dextran dialdehyde having a molecular mass of from 20,000 to 110,000 at a pH of 8.0 to 9.5 and at a temperature within the range of from 5° to 30° C. to form a covalent bond, reducing the resulting polysaccharide derivative of fibrinolysin with sodium borohydride and isolating the product.
Claim 5
Dependent Claim
66 words
A polysaccharide derivative of fibrinolysin having thrombolytic activity obtained by the steps comprising reacting fibrinolysin with dextran dialdehyde having a molecular mass of from 20,000 to 110,000 at a pH of 8.0 to 9.5 and at a temperature within the range of from 5° to 30° C. to form a covalent bond, reducing the resulting polysaccharide derivative of fibrinolysin with sodium borohydride and isolating the product.
Claim 6
Dependent Claim
14 words
The polysaccharide derivative of fibrinolysin prepared according to claim 1 which is water soluble.
Claim 7
Dependent Claim
98 words
A polysaccharide derivative of fibrinolysin which is water soluble and has prolonged thrombolytic activity, obtained by reacting fibrinolysin with dextran dialdehyde to form a covalent bond and reducing the product, said derivative being represented as follows: ##STR2## wherein FL is fibrinolysin n is 100 mol % of glucopyranose units of dextran y is 10-25 mol % of oxidized glucopyranose units of dextran p is the molar percentage of NH 2 groups of fibrinolysin bonded to dextran, and wherein the content of fibrinolysin is 10 to 30 mol % and the content of dextran 90 to 70 mol %.
Claim 8
Dependent Claim
98 words
A polysaccharide derivative of fibrinolysin which is water soluble and has prolonged thrombolytic activity, obtained by reacting fibrinolysin with dextran dialdehyde to form a covalent bond and reducing the product, said derivative being represented as follows: ##STR2## wherein FL is fibrinolysin n is 100 mol % of glucopyranose units of dextran y is 10-25 mol % of oxidized glucopyranose units of dextran p is the molar percentage of NH 2 groups of fibrinolysin bonded to dextran, and wherein the content of fibrinolysin is 10 to 30 mol % and the content of dextran 90 to 70 mol %.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-01 | 1987-10-31 | 1988-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-01 | 1991-10-31 | 1992-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-01 | 1995-10-31 | 1996-05-01 | $7700.00 | Window Closed / Lapsed |