US4020268A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Agarose containing affinity matrix materials
Assignee: Oscient Pharmaceuticals Corp
•
Filing: 1974-11-21
•
Granted: 1977-04-26
•
Expires: 1994-11-21
Abstract
Abstract A novel affinity matrix material for trypsin and trypsin-like enzymes is disclosed. Methods employing this material to isolate and/or purify crude extracts containing trypsin and trypsin-like enzymes and to store the purified enzymes obtained are also disclosed.
Inventors
- 👤 A. Hirotoshi Nishikawa
- 👤 Harry F. Hixson, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 14:51
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6429; C12N9/6427; C12Y; C12Y304/00; C12Y304/21; C12Y304/21005; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
106 words
An affinity matrix material consisting essentially of an insoluble matrix material consisting essentially of agarose covalently bonded to a leash molecule succinaminohexamethylamine which is covalently bonded to a ligand satisfying the formula: X-- A -- R wherein X is ##STR2## or ##STR3## A is selected from the group consisting of benzoyl, norvalyl, phenyl, hexyl, cyclohexyl, and pentyl and R is -- (CH 2 ) y Z, -- o(ch 2 ) y Z, -- s(ch 2 ) y Z, or -- S--S(CH 2 ) Y Z where Z is NH, COOH, I, Br, Cl COCl, NCS, NCO, SO 2 Cl, and where y is 0 to 3.
Claim 2
Dependent Claim
106 words
An affinity matrix material consisting essentially of an insoluble matrix material consisting essentially of agarose covalently bonded to a leash molecule succinaminohexamethylamine which is covalently bonded to a ligand satisfying the formula: X-- A -- R wherein X is ##STR2## or ##STR3## A is selected from the group consisting of benzoyl, norvalyl, phenyl, hexyl, cyclohexyl, and pentyl and R is -- (CH 2 ) y Z, -- o(ch 2 ) y Z, -- s(ch 2 ) y Z, or -- S--S(CH 2 ) Y Z where Z is NH, COOH, I, Br, Cl COCl, NCS, NCO, SO 2 Cl, and where y is 0 to 3.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-04-25 | 1980-10-25 | 1981-04-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-04-25 | 1984-10-25 | 1985-04-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-04-25 | 1988-10-25 | 1989-04-26 | $7700.00 | Window Closed / Lapsed |