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US4424200A
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Method for radiolabeling proteins with technetium-99m
Assignee: NUC-MEDINC A CORP OF NEW MEXICO
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Filing: 1981-03-23
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Granted: 1984-01-03
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Expires: 2001-03-23
Abstract
Abstract Proteins are radiolabeled with technetium-99m in a reducing environment comprising buffered stannous chloride wherein the tin utilized to form the stannous chloride is non-oxidized, the buffered stannous chloride is purged of oxygen and the buffer comprises an alkali metal biphthalate and an alkali metal tartrate. Proteins which have been preincubated with tin are radiolabeled with technetium-99m to form a strongly bonded, non-exchangeable radiolabeled tracer substance. The radiolabeling can be accomplished immediately after pretinning, or the pretinned proteins can be freeze-dried and the radiolabeling accomplished at some time in the future when the pretinned proteins are resolubilized in a saline solution of sodium pertechnetate - TC-99m. Pretinning can be accomplished in a reducing environment comprising stannous chloride buffered with an alkali metal biphthalate and an alkali metal tartrate, this solution having been purged of oxygen to assure that the stannous chloride is non-oxidized. When the pretinning proteins are freeze-dried, they can be utilized to prepare instant TC-99m labeling kits for use as radiopharmaceuticals.
Inventors
- 👤 David R. Crockford
- 👤 Buck A. Rhodes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 22:55
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/10; A61K51/1024; A61K2123/00
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
67 words
The process for forming a reducing composition suitable for being radiolabeled with technetium-99m which comprises incubating a source of stannous ion with a protein in the presence of a buffering composition comprising a mixture of an alkali metal biphthalate and an alkali metal tartrate having a pH between about 4.5 and 8.5 for at least about 15 hours at a temperature wherein said protein is not denatured.
Claim 2
Dependent Claim
67 words
The process for forming a reducing composition suitable for being radiolabeled with technetium-99m which comprises incubating a source of stannous ion with a protein in the presence of a buffering composition comprising a mixture of an alkali metal biphthalate and an alkali metal tartrate having a pH between about 4.5 and 8.5 for at least about 15 hours at a temperature wherein said protein is not denatured.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein said mixture is incubated for at least about 21 hours.
Claim 4
Dependent Claim
24 words
The process of any one of claims 1 or 2 wherein said buffering composition contains an alkali metal borate and and alkali metal gentisate.
Claim 5
Dependent Claim
17 words
The process of claim 1 wherein said mixture is incubated for between about 15 and 21 hours.
Claim 6
Dependent Claim
17 words
A reducing composition suitable for being radio-labeled with technetium-99m made by the process according to claim 1.
Claim 7
Dependent Claim
8 words
The composition of claim 5 which is lyophilized.
Claim 8
Dependent Claim
17 words
The composition of any one of claims 5 or 6 wherein said protein is radiolabeled with technetium-99m.
Claim 9
Dependent Claim
10 words
The composition of claim 5 wherein said protein is anti-hCG.
Claim 10
Dependent Claim
10 words
The composition of claim 5 wherein said protein is anti-hCG-beta.
Claim 11
Dependent Claim
32 words
The composition of any one of claims 5 or 6 wherein said protein, stannous ion and buffer are incubated for a period of time of between about 15 and about 20 hours.
Claim 12
Dependent Claim
40 words
The composition of any one of claims 5 or 6 wherein said protein, stannous ion and buffer are incubated for a period of time of between about 15 and about 21 hours and wherein said protein is radiolabeled with technetium-99m.
Claim 13
Dependent Claim
24 words
The composition of any one of claims 5 or 6 wherein said buffering composition contains an alkali metal borate and an alkali metal gentisate.
Claim 14
Dependent Claim
32 words
The composition of any one of claims 5 or 6 wherein said buffering composition contains an alkali metal borate and an alkali metal gentisate and wherein said protein is radiolabeled with technetium-99m.
Claim 15
Dependent Claim
24 words
The composition of any one of claims 8 or 9 wherein said buffering composition contains an alkali metal borate and an alkali metal gentisate.
Claim 16
Dependent Claim
66 words
A diagnostic kit suitable for forming a composition to be administered to a human patient which comprises a first sterile package containing a source of stannous ion and a buffering composition comprising a mixture of an alkali metal biphthalate and an alkali metal tartrate having a pH between about 4.5 and 8.5 and a second sterile package containing a protein suitable for being radiolabeled with technetium-99m.
Claim 17
Dependent Claim
66 words
A diagnostic kit suitable for forming a composition to be administered to a human patient which comprises a first sterile package containing a source of stannous ion and a buffering composition comprising a mixture of an alkali metal biphthalate and an alkali metal tartrate having a pH between about 4.5 and 8.5 and a second sterile package containing a protein suitable for being radiolabeled with technetium-99m.
Claim 18
Dependent Claim
16 words
The kit of claim 15 wherein the composition contained within the first sterile package is lyophilized.
Claim 19
Dependent Claim
26 words
The diagnostic kit of any one of claims 15 or 16 wherein said first sterile package contains an alkali metal borate and an alkali metal gentisate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-02 | 1987-07-04 | 1988-01-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-02 | 1991-07-04 | 1992-01-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-02 | 1995-07-04 | 1996-01-03 | $7700.00 | Window Closed / Lapsed |