Patent US4416994A successfully refreshed from Google Patents.
US4416994A
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Plasmid pEL7 and related cloning vectors for use in streptomyces and related organisms
Assignee: Eli Lilly and Co
•
Filing: 1981-10-19
•
Granted: 1983-11-22
•
Expires: 2001-10-19
Abstract
Abstract The present invention discloses selectable pairs of recombinant DNA cloning vectors for use in Streptomyces and related organisms.
Inventors
- 👤 Walter M. Nakatsukasa
- 👤 Jeffrey T. Fayerman
- 👤 James A. Mabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 05:37
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 72
Claim 1
Independent Claim
70 words
A novel pair of recombinant DNA cloning vectors, said pair comprising: (a) novel plasmid pEL7, and (b) a second plasmid that is functionally dependent upon plasmid pEL7, said second plasmid comprising a restriction fragment of plasmid pEL7 and one or more different DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive host cell, said host cell being susceptible to transformation, cell division, and culture.
Claim 2
Dependent Claim
70 words
A novel pair of recombinant DNA cloning vectors, said pair comprising: (a) novel plasmid pEL7, and (b) a second plasmid that is functionally dependent upon plasmid pEL7, said second plasmid comprising a restriction fragment of plasmid pEL7 and one or more different DNA segments that convey resistance to at least one antibiotic when transformed into a sensitive host cell, said host cell being susceptible to transformation, cell division, and culture.
Claim 3
Dependent Claim
40 words
The vector pair of claim 1 wherein the one or more DNA segments in the second plasmid that convey antibiotic resistance are selected from the group of segments that convey resistance to either or both of antibiotics thiostrepton and neomycin.
Claim 4
Dependent Claim
19 words
The vector pair of claim 2 wherein the DNA segment in the second plasmid conveys resistance to antibiotic thiostrepton.
Claim 5
Dependent Claim
20 words
The vector pair of claim 3 wherein the DNA segment is the 1.6 kb BamHI restriction fragment of plasmid pLR2.
Claim 6
Dependent Claim
20 words
The vector pair of claim 3 wherein the DNA segment is the 13 kb PstI restriction fragment of plasmid pLR2.
Claim 7
Dependent Claim
19 words
The vector pair of claim 2 wherein the DNA segment in the second plasmid conveys resistance to antibiotic neomycin.
Claim 8
Dependent Claim
20 words
The vector pair of claim 6 wherein the DNA segment is the 3.5 kb PstI restriction fragment of plasmid pLR1.
Claim 9
Dependent Claim
23 words
The vector pair of claim 6 wherein the DNA segment is the 3.4 kb BamHI restriction fragment of plasmid pLR1 or plasmid pLR4.
Claim 10
Dependent Claim
32 words
The vector pair of claim 2 wherein the DNA segments are the 1.6 kb BamHI restriction fragment of plasmid pLR2 and the 3.4 kb BamHI restriction fragment of plasmids pLR1 or pLR4.
Claim 11
Dependent Claim
30 words
The vector pair of claim 2 wherein the DNA segments are the 1.6 kb BamHI restriction fragment of plasmids pLR2 and the 3.5 kb PstI restriction fragment of plasmid pLR1.
Claim 12
Dependent Claim
30 words
The vector pair of claim 2 wherein the DNA segments are the 13 kb PstI restriction fragment of plasmid pLR2 and the 3.5 kb PstI restriction fragment of plasmids pLR1.
Claim 13
Dependent Claim
14 words
A single recombinant DNA cloning vector comprising the novel plasmid pEL7 of claim 1.
Claim 14
Dependent Claim
16 words
A recombinant DNA cloning vector selected from the group consisting of plasmids pLR1, pLR2, and pLR4.
Claim 15
Dependent Claim
16 words
A recombinant DNA cloning vector selected from the group consisting of plasmids pLR1, pLR2, and pLR4.
Claim 16
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 13 which is plasmid pLR1.
Claim 17
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 13 which is plasmid pLR2.
Claim 18
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 13 which is plasmid pLR4.
Claim 19
Dependent Claim
15 words
A novel recombinant DNA cloning vector comprising the functionally dependent second plasmid of claim 1.
Claim 20
Dependent Claim
26 words
The recombinant DNA cloning vector of claim 17 which is selected from the group consisting of plasmids pEL7.1, pEL7.2, pEL7.3, pEL7.4, pEL7.5, pEL7.6, pEL7.7, and pEL7.8.
Claim 21
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.1.
Claim 22
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.2.
Claim 23
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.3.
Claim 24
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.4.
Claim 25
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.5.
Claim 26
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.5.
Claim 27
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.7.
Claim 28
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 18 which is plasmid pEL7.8.
Claim 29
Dependent Claim
62 words
A novel recombinant DNA cloning vector comprising: (a) a functional E. coli replicon containing and antibiotic resistance conferring restriction fragment of an E. coli plasmid, and (b) a restriction fragment of a member of the pair of recombinant DNA cloning vectors recited in claim 1, subject to the limitation that said vector is functionally dependent upon plasmid pEL7 when transformed into Streptomyces.
Claim 30
Dependent Claim
25 words
The recombinant DNA cloning vector of claim 27 wherein the restriction fragment of the E. coli plasmid is the BamHI restriction fragment of plasmid pBR322.
Claim 31
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 28 which is plasmid pLR5.
Claim 32
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 28 which is plasmid pLR7.
Claim 33
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 28 which is plasmid pLR9.
Claim 34
Dependent Claim
12 words
The recombinant DNA cloning vector of claim 28 which is plasmid pLR11.
Claim 35
Dependent Claim
33 words
A transformed restrictionless host cell selected from the group consisting of restrictionless cells of Streptomyces, Streptosporangium, Actinoplanes, Nocardia, Micromonospora, Bacillus, and Staphylococcus comprising a pair of recombinant DNA cloning vectors of claim 1.
Claim 36
Dependent Claim
39 words
The transformed restrictionless host cell of claim 33 in which the pair of recombinant DNA cloning vectors comprises plasmid pEL7 and a plasmid selected from the group consisting of plasmids pEL7.1, pEL7.2, pEL7.3, pEL7.4, pEL7.5, pEL7.6, pEL7.7, and pEL7.8.
Claim 37
Dependent Claim
31 words
A transformed restrictionless host cell selected from the group consisting of restrictionless cells of Streptomyces, Streptosporangium, Actinoplanes, Nocardia, Micromonospora, Bacillus, and Staphylococcus comprising a recombinant DNA cloning vector of claim 12.
Claim 38
Dependent Claim
10 words
A transformed host cell of claim 33 which is Streptomyces.
Claim 39
Dependent Claim
10 words
The transformed host cell of claim 34 which is Streptosporangium.
Claim 40
Dependent Claim
10 words
The transformed host cell of claim 34 which is Actinoplanes.
Claim 41
Dependent Claim
10 words
The transformed host cell of claim 34 which is Nocardia.
Claim 42
Dependent Claim
10 words
The transformed host cell of claim 34 which is Micromonospora.
Claim 43
Dependent Claim
10 words
The transformed host cell of claim 34 which is Bacillus.
Claim 44
Dependent Claim
10 words
The transformed host cell of claim 34 which is Staphylococcus.
Claim 45
Dependent Claim
10 words
The transformed host cell of claim 34 which is Streptomyces.
Claim 46
Dependent Claim
9 words
The Streptomyces of claim 43 which is Streptomyces fradiae.
Claim 47
Dependent Claim
9 words
The Streptomyces of claim 43 which is Streptomyces aureofaciens.
Claim 48
Dependent Claim
9 words
The Streptomyces of claim 43 which is Streptomyces coelicolor.
Claim 49
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.1.
Claim 50
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.2.
Claim 51
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.3.
Claim 52
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.4.
Claim 53
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.5.
Claim 54
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.6.
Claim 55
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.7.
Claim 56
Dependent Claim
12 words
The transformed host cell of claim 34 which is Streptomyces ambofaciens/pEL7, pEL7.8.
Claim 57
Dependent Claim
11 words
The transformed host cell of claim 35 which is Streptomyces ambofaciens/pEL7.
Claim 58
Dependent Claim
44 words
A transformed restrictionless host cell selected from the group consisting of E. coli and Streptomyces comprising the recombinant DNA cloning vector of claim 27 subject to the limitation that when said transformed host cell is Streptomyces said vector is functionally dependent upon plasmid pEL7.
Claim 59
Dependent Claim
24 words
The transformed host cell of claim 56 wherein the restriction fragment of the E. coli plasmid is the BamHI restriction fragment of plasmid pBR322.
Claim 60
Dependent Claim
11 words
The transformed host cell of claim 57 which is E. coli.
Claim 61
Dependent Claim
13 words
The transformed host cell of claim 57 which is E. coli K12 HB101/pLR7.
Claim 62
Dependent Claim
13 words
The transformed host cell of claim 57 which is E. coli K12 HB101/pLR9.
Claim 63
Dependent Claim
13 words
The transformed host cell of claim 57 which is E. coli K12 HB101/pLR11.
Claim 64
Dependent Claim
13 words
The transformed host cell of claim 57 which is E. coli K12 HB101/pLR5.
Claim 65
Dependent Claim
13 words
The transformed host cell of claim 57 which is E. coli K12 HB101/pLR6.
Claim 66
Dependent Claim
10 words
The transformed host cell of claim 57 which is Streptomyces.
Claim 67
Dependent Claim
22 words
The transformed host cell of claim 64 which is selected from the group consisting of Streptomyces ambofaciens/pEL7, pLR5, and Streptomyces ambofaciens/pEL7, pLR7.
Claim 68
Dependent Claim
12 words
The transformed host cell of claim 64 which is Streptomyces ambofaciens/pEL7, pLR5.
Claim 69
Dependent Claim
12 words
The transformed host cell of claim 64 which is Streptomyces ambofaciens/pEL7, pLR7.
Claim 70
Dependent Claim
13 words
The transformed restrictionless host cell of claim 56 which is E. coli K12.
Claim 71
Dependent Claim
15 words
The transformed restrictionless host cell of claim 56 which is E. coli K12 HB 101.
Claim 72
Dependent Claim
12 words
The transformed restrictionless host cell of claim 56 which is Streptomyces ambofaciens.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-21 | 1987-05-23 | 1987-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-21 | 1991-05-23 | 1991-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-21 | 1995-05-23 | 1995-11-22 | $7700.00 | Window Closed / Lapsed |