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US4343906A Expired / Lapsed US ⏱️ 0.0 years remaining

Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same

Assignee: Pharmacia and Upjohn Co
•
Filing: 1979-08-21
•
Granted: 1982-08-10
•
Expires: 1999-08-21
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Abstract

Abstract A process for cloning DNA into a suitable host, which comprises fragmenting said DNA to obtain fragmented DNA, ligating said fragmented DNA into a suitable vector to obtain chimeric (hybrid) DNA, and transforming said chimeric DNA into said ultimate host. By this process, the useful chemical plasmid pUC3, which is obtainable from a biologically pure culture of the microorganism Streptomyces sp. 3022a, NRRL 11441, is cloned into the well-known bacterium E. coli HB101. This cloning of pUC3 into E. coli HB101 enables the production of large amounts of plasmid pUC3 DNA. pUC3 is useful as a cloning vehicle in recombinant DNA work. For example, using recombinant DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUC3 and the resulting plasmid can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.

Inventors

  • 👤 Fritz Reusser
  • 👤 Vedpal S. Malik

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-02 09:59
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76

Claims Analysis

Total Claims: 12
Claim 1
Independent Claim 11 words
E. coli HB101 (pUC1009) having the desposit accession number NRRL B-12005.
Claim 2
Dependent Claim 11 words
E. coli HB101 (pUC1009) having the desposit accession number NRRL B-12005.
Claim 3
Dependent Claim 11 words
E. coli HB101 (pUC1010) having the deposit accession number NRRL B-12006.
Claim 4
Dependent Claim 11 words
E. coli HB101 (pUC1010) having the deposit accession number NRRL B-12006.
Claim 5
Dependent Claim 11 words
E. coli HB101 (pUC1011) having the deposit accession number NRRL B-12007.
Claim 6
Dependent Claim 11 words
E. coli HB101 (pUC1011) having the deposit accession number NRRL B-12007.
Claim 7
Dependent Claim 48 words
Hybrid plasmid pUC1009 characterized as follows: (a) it is a hybrid plasmid which has the entire nucleotide sequence of plasmid pBR322 and a foreign DNA insert at the BamH-1 site of pBR322; (b) said DNA insert being the 19 kb fragment of plasmid pUC3 shown in the drawing.
Claim 8
Dependent Claim 48 words
Hybrid plasmid pUC1009 characterized as follows: (a) it is a hybrid plasmid which has the entire nucleotide sequence of plasmid pBR322 and a foreign DNA insert at the BamH-1 site of pBR322; (b) said DNA insert being the 19 kb fragment of plasmid pUC3 shown in the drawing.
Claim 9
Dependent Claim 48 words
Hybrid plasmid pUC1010 characterized as follows: (a) it is a hybrid plasmid which has the entire nucleotide sequence of plasmid pBR322 and a foreign DNA insert at the BAMH-1 site of pBR322; (b) said DNA insert being the 5.7 kb fragment of plasmid pUC3 shown in the drawing.
Claim 10
Dependent Claim 48 words
Hybrid plasmid pUC1010 characterized as follows: (a) it is a hybrid plasmid which has the entire nucleotide sequence of plasmid pBR322 and a foreign DNA insert at the BAMH-1 site of pBR322; (b) said DNA insert being the 5.7 kb fragment of plasmid pUC3 shown in the drawing.
Claim 11
Dependent Claim 51 words
Hybrid plasmid pUC1011 characterized as follows: (a) it is a hybrid plasmid which has the entire nucleotide sequence of plasmid pBR322 and a foreign DNA insert at the BamH-1 site of pBR322; (b) said DNA insert being the 5.7, 4.1 and 2.7 kb fragments of plasmid pUC3 shown in the drawing.
Claim 12
Dependent Claim 51 words
Hybrid plasmid pUC1011 characterized as follows: (a) it is a hybrid plasmid which has the entire nucleotide sequence of plasmid pBR322 and a foreign DNA insert at the BamH-1 site of pBR322; (b) said DNA insert being the 5.7, 4.1 and 2.7 kb fragments of plasmid pUC3 shown in the drawing.

References Cited (Backward Citations)

US4237224A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1985-08-09 1986-02-08 1986-08-10 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1989-08-09 1990-02-08 1990-08-10 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1993-08-09 1994-02-08 1994-08-10 $7700.00 Window Closed / Lapsed

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