US4340674A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cointegrate plasmids and their construction from plasmids of Escherichia and Streptomyces
Assignee: Pharmacia and Upjohn Co
•
Filing: 1980-07-23
•
Granted: 1982-07-20
•
Expires: 2000-07-23
Abstract
Abstract Novel chemical compounds, cointegrate plasmids pUC1012 and pUC1013, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6, and plasmids pUC1015 and pUC1022 which are obtained by restructuring plasmid pUC1012, and plasmids pUC1016 and pUC1023 which are obtained by restructuring plasmid pUC1013. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.
Inventors
- 👤 Jack J. Manis
- 👤 Sarah K. Highlander
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:05
CPC Classifications
C; C12; C12N; C12N15/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/886
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
11 words
E. coli CSH50 (pUC1012) having the deposit accession number NRRL B-12106.
Claim 2
Dependent Claim
11 words
E. coli CSH50 (pUC1012) having the deposit accession number NRRL B-12106.
Claim 3
Dependent Claim
11 words
E. coli CSH50 (pUC1013) having the deposit accession number NRRL B-12107.
Claim 4
Dependent Claim
11 words
E. coli CSH50 (pUC1013) having the deposit accession number NRRL B-12107.
Claim 5
Dependent Claim
16 words
Cointegrate plasmid pUC1012, characterized as shown by the restriction map in FIG. 2 of the drawings.
Claim 6
Dependent Claim
16 words
Cointegrate plasmid pUC1012, characterized as shown by the restriction map in FIG. 2 of the drawings.
Claim 7
Dependent Claim
16 words
Cointegrate plasmid pUC1013 characterized as shown by the restriction map in FIG. 3 of the drawings.
Claim 8
Dependent Claim
16 words
Cointegrate plasmid pUC1013 characterized as shown by the restriction map in FIG. 3 of the drawings.
Claim 9
Dependent Claim
40 words
A process for preparing cointegrate plasmids pUC1012 and pUC1013 which comprises: (a) linearizing plasmids pBR322 and pUC6 to obtain linear plasmid DNA; and, (b) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1012 and pUC1013.
Claim 10
Dependent Claim
40 words
A process for preparing cointegrate plasmids pUC1012 and pUC1013 which comprises: (a) linearizing plasmids pBR322 and pUC6 to obtain linear plasmid DNA; and, (b) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1012 and pUC1013.
Claim 11
Dependent Claim
50 words
A process for cloning plasmid pUC6 into a suitable bacterium which comprises: (a) linearizing plasmids pBR322 and pUC6 to obtain linear plasmid DNA; (b) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1012 and pUC1013; and, (c) transforming said cointegerate plasmids into said suitable bacterium.
Claim 12
Dependent Claim
50 words
A process for cloning plasmid pUC6 into a suitable bacterium which comprises: (a) linearizing plasmids pBR322 and pUC6 to obtain linear plasmid DNA; (b) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1012 and pUC1013; and, (c) transforming said cointegerate plasmids into said suitable bacterium.
Claim 13
Dependent Claim
13 words
A process, according to claim 6, wherein said bacterium is E. coli CSH50.
Claim 14
Dependent Claim
11 words
E. coli CSH50 (pUC1015) having the deposit accession number NRRL B-12150.
Claim 15
Dependent Claim
11 words
E. coli CSH50 (pUC1015) having the deposit accession number NRRL B-12150.
Claim 16
Dependent Claim
11 words
E. coli CSH50 (pUC1016) having the deposit accession number NRRL B-12151.
Claim 17
Dependent Claim
11 words
E. coli CSH50 (pUC1016) having the deposit accession number NRRL B-12151.
Claim 18
Dependent Claim
15 words
Plasmid pUC1015, characterized as shown by the restriction map in FIG. 4 of the drawings.
Claim 19
Dependent Claim
15 words
Plasmid pUC1015, characterized as shown by the restriction map in FIG. 4 of the drawings.
Claim 20
Dependent Claim
15 words
Plasmid pUC1016 characterized as shown by the restriction map in FIG. 5 of the drawings.
Claim 21
Dependent Claim
15 words
Plasmid pUC1016 characterized as shown by the restriction map in FIG. 5 of the drawings.
Claim 22
Dependent Claim
37 words
A process for preparing plasmid pUC1015 which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; and, (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1015.
Claim 23
Dependent Claim
37 words
A process for preparing plasmid pUC1015 which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; and, (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1015.
Claim 24
Dependent Claim
37 words
A process for preparing plasmid pUC1016 which comprises: (a) digestion of pUC1013 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; and, (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1016.
Claim 25
Dependent Claim
37 words
A process for preparing plasmid pUC1016 which comprises: (a) digestion of pUC1013 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; and, (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1016.
Claim 26
Dependent Claim
49 words
A process for cloning plasmid pUC1015 into a suitable bacterium which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1015; and, (c) transforming said plasmid into said suitable bacterium.
Claim 27
Dependent Claim
49 words
A process for cloning plasmid pUC1015 into a suitable bacterium which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1015; and, (c) transforming said plasmid into said suitable bacterium.
Claim 28
Dependent Claim
13 words
A process, according to claim 14, wherein said bacterium is E. coli CSH50.
Claim 29
Dependent Claim
49 words
A process for cloning plasmid pUC1016 into a suitable bacterium which comprises: (a) digestion of pUC1013 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1016; and, (c) transforming said plasmid into said suitable bacterium.
Claim 30
Dependent Claim
49 words
A process for cloning plasmid pUC1016 into a suitable bacterium which comprises: (a) digestion of pUC1013 DNA with restriction endonuclease XhoI to obtain fragmented linear plasmid DNA; (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1016; and, (c) transforming said plasmid into said suitable bacterium.
Claim 31
Dependent Claim
13 words
A process, according to claim 16, wherein said bacterium is E. coli CSH50.
Claim 32
Dependent Claim
11 words
E. coli RR1 (pUC1022) having the deposit accession number NRRL B-12183.
Claim 33
Dependent Claim
11 words
E. coli RR1 (pUC1022) having the deposit accession number NRRL B-12183.
Claim 34
Dependent Claim
11 words
E. coli RR1 (pUC1023) having the deposit accession number NRRL B-12184.
Claim 35
Dependent Claim
11 words
E. coli RR1 (pUC1023) having the deposit accession number NRRL B-12184.
Claim 36
Dependent Claim
15 words
Plasmid pUC1022, characterized as shown by the restriction map in FIG. 10 of the drawings.
Claim 37
Dependent Claim
15 words
Plasmid pUC1022, characterized as shown by the restriction map in FIG. 10 of the drawings.
Claim 38
Dependent Claim
15 words
Plasmid pUC1023, characterized as shown by the restriction map in FIG. 11 of the drawings.
Claim 39
Dependent Claim
15 words
Plasmid pUC1023, characterized as shown by the restriction map in FIG. 11 of the drawings.
Claim 40
Dependent Claim
37 words
A process for preparing plasmid pUC1022 which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; and, (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1022.
Claim 41
Dependent Claim
37 words
A process for preparing plasmid pUC1022 which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; and, (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1022.
Claim 42
Dependent Claim
41 words
A process for preparing plasmid pUC1023 which comprises: (a) digestion of pUC1013 DNA with restrictuion endonuclease PvuII to obtain fragmented linear plasmid DNA; and, (b) ligating the fragment containing the ampicillin resistance gene of said plasmid DNA to obtain plasmid pUC1023.
Claim 43
Dependent Claim
41 words
A process for preparing plasmid pUC1023 which comprises: (a) digestion of pUC1013 DNA with restrictuion endonuclease PvuII to obtain fragmented linear plasmid DNA; and, (b) ligating the fragment containing the ampicillin resistance gene of said plasmid DNA to obtain plasmid pUC1023.
Claim 44
Dependent Claim
49 words
A process for cloning plasmid pUC1022 into a suitable bacterium which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1022; and, (c) transforming said plasmid into said suitable bacterium.
Claim 45
Dependent Claim
49 words
A process for cloning plasmid pUC1022 into a suitable bacterium which comprises: (a) digestion of pUC1012 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; (b) ligating the largest fragment of said plasmid DNA to obtain plasmid pUC1022; and, (c) transforming said plasmid into said suitable bacterium.
Claim 46
Dependent Claim
13 words
A process, according to claim 24, wherein said bacterium is E. coli RR1.
Claim 47
Dependent Claim
53 words
A process for cloning plasmid pUC1023 into a suitable bacterium which comprises: (a) digestion of pUC1013 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; (b) ligating the fragment containing the ampicillin resistance gene of said plasmid DNA to obtain plasmid pUC1023; and, (c) transforming said plasmid into said suitable bacterium.
Claim 48
Dependent Claim
53 words
A process for cloning plasmid pUC1023 into a suitable bacterium which comprises: (a) digestion of pUC1013 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; (b) ligating the fragment containing the ampicillin resistance gene of said plasmid DNA to obtain plasmid pUC1023; and, (c) transforming said plasmid into said suitable bacterium.
Claim 49
Dependent Claim
13 words
A process, according to claim 26, wherein said bacterium is E. coli RR1.
References Cited (Backward Citations)
US4237224A
Cited
US4273875A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-19 | 1986-01-18 | 1986-07-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-19 | 1990-01-18 | 1990-07-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-19 | 1994-01-18 | 1994-07-20 | $7700.00 | Window Closed / Lapsed |