Abstract A complex of polyriboinosinic-polyribocytidylic acid (poly I:C) with poly-L-lysine hydrobromide ([lysine.HBr]n) having a defined molecular weight is prepared which is about 5-15 times as resistant to hydrolysis by pancreatic ribonuclease as the parent poly I:C. A pharmaceutical solution, containing a relatively high concentration of the complex, prepared as described, induces significant antiviral levels of serum interferon in monkeys under conditions in which poly I:C itself induces little or no interferon. An important feature of this invention is that the product is a soluble material requiring no special solubilizing agent, thereby facilitating the preparation of solutions having the desired concentration. The product will hereinafter be designated as poly I:C/poly-L-lysine.
An interferon-inducing composition comprising a solution in a pharmaceutically acceptable aqueous carrier, of a complex of poly I:C/poly-L-lysine hydrobromide or hydrochloride, the concentration in solution of the poly I:C being from about 1 to about 2 mg/ml, the ratio by dry weight of the poly I:C to the poly-L-lysine hydrobromide or hydrochloride being about 1 to about 0.3, or a molar ratio of 2:1; the poly I having a S w 20° of 11-20, and an E 1% at 248 nm of 240-280; the poly C having a S w 20° of 5-7 and an E 1% at 248 nm of 175-185; and the poly-L-lysine having a molecular weight of from about 10,000 to about 70,000 daltons.
Claim 2
Dependent Claim
117 words
An interferon-inducing composition comprising a solution in a pharmaceutically acceptable aqueous carrier, of a complex of poly I:C/poly-L-lysine hydrobromide or hydrochloride, the concentration in solution of the poly I:C being from about 1 to about 2 mg/ml, the ratio by dry weight of the poly I:C to the poly-L-lysine hydrobromide or hydrochloride being about 1 to about 0.3, or a molar ratio of 2:1; the poly I having a S w 20° of 11-20, and an E 1% at 248 nm of 240-280; the poly C having a S w 20° of 5-7 and an E 1% at 248 nm of 175-185; and the poly-L-lysine having a molecular weight of from about 10,000 to about 70,000 daltons.
Claim 3
Dependent Claim
15 words
The composition of claim 1 in which the poly I:C concentration is about 1 mg/ml.
Claim 4
Dependent Claim
14 words
The composition of claim 1 in which the poly-L-lysine is in the hydrobromide form.
Claim 5
Dependent Claim
22 words
The composition of claim 3 in which the molecular weight of the poly-L-lysine hydrobromide is from about 10,000 to about 35,000 daltons.
Claim 6
Dependent Claim
26 words
The composition of claim 1 wherein the poly I has a S w 20° of about 19 and an E 1% at 248 nm of 240.
Claim 7
Dependent Claim
24 words
The composition of claim 1 wherein poly C has a S w 20° of about 5 and an E 1% at 248 of 177.