US4024222A
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Nucleic acid complexes
Assignee: Johns Hopkins University
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Filing: 1973-10-30
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Granted: 1977-05-17
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Expires: 1993-10-30
Abstract
Abstract The present invention relates to the induction of interferon production in the cells of living organisms, including human beings. According to the invention, nucleic acid complexes, such as the polyriboinosinate and polycytidylate complex (rIn.rCn), are modified to yield unpaired bases (uracil or guanine) along the polycytidylate strand which render the complexes more readily hydrolyzable by nucleases present in living cells. The modified complexes retain their ability to stimulate interferon release by the cells but are rendered more vulnerable to destruction within the cells, the modified complexes being significantly less toxic than the original complexes. In addition, polyinosinate strand now has been prepared to contain 5-16% 2'-O-methyl inosinate residues, designated as (rI5-20,2'-MeI)n. The new complex (rI5-20,2'-MeI)n.rCn, exhibits 100-fold more activity than rIn.rCn as an interferon inducer in human cells.
Inventors
- 👤 Paul O. P. Ts'o
- 👤 William A. Carter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:52
CPC Classifications
C; C07; C07H; C07H21/00; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
28 words
A copolynucleotide selected from the group consisting of poly(C n ,U) and poly(C n ,G) wherein n is an integer having a value of from 4 to 29.
Claim 2
Dependent Claim
28 words
A copolynucleotide selected from the group consisting of poly(C n ,U) and poly(C n ,G) wherein n is an integer having a value of from 4 to 29.
Claim 3
Dependent Claim
9 words
A copolynucleotide as in claim 1, poly(C 4 ,U).
Claim 4
Dependent Claim
9 words
A copolynucleotide as in claim 1, poly(C 7 ,U).
Claim 5
Dependent Claim
9 words
A copolynucleotide as in claim 1, poly(C 13 ,U).
Claim 6
Dependent Claim
9 words
A copolynucleotide as in claim 1, poly(C 22 ,U).
Claim 7
Dependent Claim
9 words
A copolynucleotide as in claim 1, poly(C 20 ,G).
Claim 8
Dependent Claim
9 words
A copolynucleotide as in claim 1, poly(C 29 ,G).
Claim 9
Dependent Claim
28 words
A complex selected from the group consisting of poly(I).poly(C n ,U) and poly(I).poly(C n ,G) wherein n is an integer having a value of from 4 to 29.
Claim 10
Dependent Claim
28 words
A complex selected from the group consisting of poly(I).poly(C n ,U) and poly(I).poly(C n ,G) wherein n is an integer having a value of from 4 to 29.
Claim 11
Dependent Claim
9 words
A complex as in claim 8, poly(I).poly(C 4 ,U).
Claim 12
Dependent Claim
9 words
A complex as in claim 8, poly(I).poly(C 7 ,U).
Claim 13
Dependent Claim
9 words
A complex as in claim 8, poly(I).poly(C 13 ,U).
Claim 14
Dependent Claim
9 words
A complex as in claim 8, poly(I).poly(C 22 ,U).
Claim 15
Dependent Claim
9 words
A complex as in claim 8, poly(I).poly(C 20 ,G).
Claim 16
Dependent Claim
9 words
A complex as in claim 8, poly(I).poly(C 29 ,G).
Claim 17
Dependent Claim
15 words
A composition of matter consisting of poly (I) . poly(C p) 23 G > p.
Claim 18
Dependent Claim
15 words
A composition of matter consisting of poly (I) . poly(C p) 23 G > p.
Claim 19
Independent Claim
122 words
A method for producing a chemical site on a double-stranded, complementary polynucleotide duplex of polyinosinic acid and polycytidylic acid which renders the molecule more readily hydrolyzable by enzymatic activity in the cells of a living organism comprising the step of preventing pairing of bases between the strands of the molecule, said prevention of pairing being accomplished by substituting for cytidylic acid (C) or inosinic acid (I) a nucleotide selected from the group consisting of uridylic acid (U) and guanylic acid (G) so as to produce a complex selected from the group consisting of poly (C n ,U), poly (C n ,G), poly (I).poly (C n ,U) and poly (I).poly(C n ,G) wherein n is an integer having a value 4 to 29.
Claim 20
Independent Claim
122 words
A method for producing a chemical site on a double-stranded, complementary polynucleotide duplex of polyinosinic acid and polycytidylic acid which renders the molecule more readily hydrolyzable by enzymatic activity in the cells of a living organism comprising the step of preventing pairing of bases between the strands of the molecule, said prevention of pairing being accomplished by substituting for cytidylic acid (C) or inosinic acid (I) a nucleotide selected from the group consisting of uridylic acid (U) and guanylic acid (G) so as to produce a complex selected from the group consisting of poly (C n ,U), poly (C n ,G), poly (I).poly (C n ,U) and poly (I).poly(C n ,G) wherein n is an integer having a value 4 to 29.
Claim 21
Dependent Claim
27 words
A composition of matter comprising an inosinic acid - 2'-O-methylinosinic acid copolymer. polycytidylic acid complex wherein said copolymer contains from 5 to 20% of 2'-O-methyl-inosinic acid units.
Claim 22
Dependent Claim
27 words
A composition of matter comprising an inosinic acid - 2'-O-methylinosinic acid copolymer. polycytidylic acid complex wherein said copolymer contains from 5 to 20% of 2'-O-methyl-inosinic acid units.
Claim 23
Dependent Claim
19 words
The composition of matter of claim 17 wherein said copolymer contains from 5 to 16% of 2'-O-methylinosinic acid units.
Claim 24
Dependent Claim
93 words
A therapeutic composition of matter comprising an effective amount of a double-stranded, complementary polynucleotide duplex of polyinosinic acid and polycytidylic acid wherein cytidylic acid (C) is partially substituted by a nucleotide selected from the group consisting of uridylic acid (U) and guanylic acid (G) so as to produce a complex selected from the group consisting of poly (C n ,U), poly (C n ,G), poly (I). poly (C n ,U) and poly (I). poly (C n ,G) wherein n is an integer having a value 4 to 29, together with an inert carrier.
Claim 25
Dependent Claim
93 words
A therapeutic composition of matter comprising an effective amount of a double-stranded, complementary polynucleotide duplex of polyinosinic acid and polycytidylic acid wherein cytidylic acid (C) is partially substituted by a nucleotide selected from the group consisting of uridylic acid (U) and guanylic acid (G) so as to produce a complex selected from the group consisting of poly (C n ,U), poly (C n ,G), poly (I). poly (C n ,U) and poly (I). poly (C n ,G) wherein n is an integer having a value 4 to 29, together with an inert carrier.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-05-16 | 1980-11-15 | 1981-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-05-16 | 1984-11-15 | 1985-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-05-16 | 1988-11-15 | 1989-05-17 | $7700.00 | Window Closed / Lapsed |