Patent US5537553A successfully refreshed from Google Patents.
Patent US4388233A successfully refreshed from Google Patents.
US4388233A
Expired / Lapsed
US
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Synthetic substrates for enzyme analysis
Assignee: University of California
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Filing: 1981-05-15
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Granted: 1983-06-14
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Expires: 2001-05-15
Abstract
Abstract Synthetic substrates are provided which may be represented as A-D. The A moiety thereof includes an amino acid, polypeptide, or derivative thereof. The D moiety thereof includes 7-amino coumarin derivatives having an electron withdrawing substituent group at the 3 position carbon or fused between the 3 and 4 position carbons.
Inventors
- 👤 Eugene R. Bissell
- 👤 Alexander R. Mitchell
- 👤 Karen W. Pearson
- 👤 Robert E. Smith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 19:35
CPC Classifications
C; C07; C07D; C07D311/00; C07D311/02; C07D311/04; C07D311/06; C07D311/08; C07D311/16; C07D311/78; C07D311/80; C07K; C07K5/00; C07K5/04; C07K5/06; C07K5/06086; C07K5/08; C07K5/0802; C07K5/0804; C07K5/0806; C07K5/0815; C12; C12Q; C12Q1/00; C12Q1/34; C12Q2337/00; C12Q2337/20
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
116 words
A synthetic substrate, useful for enzyme analysis, represented by A-D comprising: an A moiety and a D moiety, said A and D moieties of said A-D bonded together by an enzyme hydrolyzable bond, said A moiety selected from the group consisting of Leu, Ala, Arg, Lys-Ala, Gly-Gly-Arg, salt forms thereof and blocking groups thereon, said A moiety determining an enzyme specificity of at least one enzyme for said synthetic substrate, said D moiety being a coumarin derivative defining a 3 position carbon and a 4 position carbon and having an electron withdrawing group substituted at said 3 position carbon or fused at said 3 and 4 position carbons and selected from the group consisting of ##STR6##
Claim 2
Dependent Claim
116 words
A synthetic substrate, useful for enzyme analysis, represented by A-D comprising: an A moiety and a D moiety, said A and D moieties of said A-D bonded together by an enzyme hydrolyzable bond, said A moiety selected from the group consisting of Leu, Ala, Arg, Lys-Ala, Gly-Gly-Arg, salt forms thereof and blocking groups thereon, said A moiety determining an enzyme specificity of at least one enzyme for said synthetic substrate, said D moiety being a coumarin derivative defining a 3 position carbon and a 4 position carbon and having an electron withdrawing group substituted at said 3 position carbon or fused at said 3 and 4 position carbons and selected from the group consisting of ##STR6##
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-13 | 1986-12-13 | 1987-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-13 | 1990-12-13 | 1991-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-13 | 1994-12-13 | 1995-06-14 | $7700.00 | Window Closed / Lapsed |