US3862011A
Expired / Lapsed
US
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Method for quantitatively determining enzyme concentrations in homogenates
Assignee: Individual
•
Filing: 1973-06-18
•
Granted: 1975-01-21
•
Expires: 1993-06-18
Abstract
Abstract Various amino acid derivatives of 4-methoxy-2-naphthylamine are disclosed, as is a process for determining the concentrations of enzymes in homogenates through the use of a solution containing, among other compounds, said amino acid derivatives.
Inventors
- 👤 Robert E Smith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 08:05
CPC Classifications
C; C07; C07K; C07K5/00; C07K5/04; C07K5/08; C07K5/0802; C07K5/0804; C07K5/0806; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
24 words
The method of claim 1 in which a denaturing compound is added to the hydrolysed mixture of substrate and homogenate to stop the hydrolysis.
Claim 2
Dependent Claim
11 words
The method of claim 1 in which the substrate is carbobenzoxydiglycyl-L-arginyl-4-methoxy-2naphthylamide.
Claim 3
Dependent Claim
15 words
The method of claim 1 in which a buffer is also added to the homogenate.
Claim 4
Dependent Claim
14 words
The method of claim 2 in which a buffer is added to the homogenate.
Claim 5
Dependent Claim
11 words
The method of claim 5 in which the substrate is carbobenzoxydiglycyl-L-arginyl-4-methoxy-2-naphthylamide.
References Cited (Backward Citations)
US3591459A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-01-20 | 1978-07-22 | 1979-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-01-20 | 1982-07-22 | 1983-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-01-20 | 1986-07-22 | 1987-01-21 | $7700.00 | Window Closed / Lapsed |