US4371514A
Expired / Lapsed
US
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Radioimmunoassay of pterins and novel pterin derivatives useful therefor
Assignee: Fujifilm RI Pharma Co Ltd
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Filing: 1980-07-07
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Granted: 1983-02-01
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Expires: 2000-07-07
Abstract
Abstract Pterin derivatives are described having the formula (I) ##STR1## wherein R represents a hydroxyphenyl group, a radioiodinated hydroxyphenyl group, a tyraminocarbonyl group, a radioiodinated tyraminocarbonyl group, a proteinocarbonyl group or a carboxy group; Q represents a straight or branched chain alkylene group having 1 to 6 carbon atoms; and R 6 and R 7 each represents a hydrogen, an alkyl group having from 1 to 6 carbon atoms, a hydroxyalkyl group having from 1 to 6 carbon atoms; and a radioimmunoassay method is described using a radioiodinated 4-hydroxy-2-tyraminopteridine derivative or a radioiodinated 4-hydroxy-2-tyraminocarbonylalkylaminopteridine derivative as the tracer.
Inventors
- 👤 Toshiharu Nagatsu
- 👤 Takeshi Kato
- 👤 Tokio Yamaguchi
- 👤 Miki Akino
- 👤 Sadao Matsuura
- 👤 Takashi Sugimoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:07
CPC Classifications
C; C07; C07D; C07D239/00; C07D239/02; C07D239/24; C07D239/28; C07D239/46; C07D239/48; C07D475/00; C07D475/02; C07D475/04; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/534; G01N33/82
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
71 words
A compound of the formula ##STR8## wherein R represents a hydroxyphenyl, a radioiodinated hydroxyphenyl, a tyraminocarbonyl, a radioiodinated tyraminocarbonyl, a proteinocarbonyl group or a carboxyl; Q represents a straight or branched chain alkylene group having from 1 to 6 carbon atoms; and R 6 and R 7 each represents hydrogen, and alkyl group having from 1 to 6 carbon atoms, or a hydroxyalkyl group having from 1 to 6 carbon atoms.
Claim 2
Dependent Claim
71 words
A compound of the formula ##STR8## wherein R represents a hydroxyphenyl, a radioiodinated hydroxyphenyl, a tyraminocarbonyl, a radioiodinated tyraminocarbonyl, a proteinocarbonyl group or a carboxyl; Q represents a straight or branched chain alkylene group having from 1 to 6 carbon atoms; and R 6 and R 7 each represents hydrogen, and alkyl group having from 1 to 6 carbon atoms, or a hydroxyalkyl group having from 1 to 6 carbon atoms.
Claim 3
Dependent Claim
12 words
A compound as in claim 1, wherein the compound is radioiodinated 4-hydroxy-2-[2-(4-hydroxyphenyl)ethylamino]-6-(L-erythro-1,2-dihydroxypropyl)pteridine.
Claim 4
Dependent Claim
12 words
A compound as in claim 1, wherein the compound is radioiodinated 2-[5-(tyraminocarbonyl)pentylamino]-4-hydroxy-6-(L-erythro-1,2-dihydroxypropyl)pteridine.
Claim 5
Dependent Claim
12 words
A compound as in claim 1, wherein the compound is 2-(5-carboxypentylamino)-4-hydroxy-6-(L-erythro-1,2-dihydroxypropyl)pteridine-BSA conjugate.
Claim 6
Dependent Claim
11 words
A compound as in claim 1, wherein the compound is 2-(5-carboxypentylamino)-4-hydroxy-6-(L-erythro-1,2-dihydroxypropyl)pteridine.
Claim 7
Dependent Claim
12 words
A compound as in claim 1, wherein the compound is radioiodinated 4-hydroxy-2-[2-(4-hydroxyphenyl)ethylamino]-6-(L-erythro-1,2,3-trihydroxypropyl)pteridine.
Claim 8
Dependent Claim
12 words
A compound as in claim 1, wherein the compound is radioiodinated 2-[5-(tyraminocarbonyl)pentylamino]-4-hydroxy-6-(L-erythro-1,2,3-trihydroxypropyl)pteridine.
Claim 9
Dependent Claim
12 words
A compound as in claim 1, wherein the compound is 2-(5-carboxypentylamino)-4-hydroxy-6-(L-erythro-1,2,3-trihydroxypropyl)-pteridine-BSA conjugate.
Claim 10
Dependent Claim
11 words
A compound as in claim 1, wherein the compound is 2-(5-carboxypentylamino)-4-hydroxy-6-(L-erythro-1,2,3-trihydroxypropyl)pteridine.
Claim 11
Independent Claim
117 words
A method of radioimmunoassay for determining pterines, comprising mixing an unknown sample or a known amount of a standard with a labeled compound of the formula ##STR9## wherein R represents a radioiodinated hydroxyphenyl group or a radioiodinated tyraminocarbonyl group; Q represents a straight or branched chain alkylene group having from 1 to 6 carbon atoms; and R 6 and R 7 each represents hydrogen, an alkyl group having from 1 to 6 carbon atoms, or a hydroxyalkyl group having from 1 to 6 carbon atoms, and anti-pterines antibody, separating bound labeled compound from remaining free labeled compound, and counting the radiation emitted from either the bound labeled compound or the free labeled compound in a gamma counter.
Claim 12
Independent Claim
117 words
A method of radioimmunoassay for determining pterines, comprising mixing an unknown sample or a known amount of a standard with a labeled compound of the formula ##STR9## wherein R represents a radioiodinated hydroxyphenyl group or a radioiodinated tyraminocarbonyl group; Q represents a straight or branched chain alkylene group having from 1 to 6 carbon atoms; and R 6 and R 7 each represents hydrogen, an alkyl group having from 1 to 6 carbon atoms, or a hydroxyalkyl group having from 1 to 6 carbon atoms, and anti-pterines antibody, separating bound labeled compound from remaining free labeled compound, and counting the radiation emitted from either the bound labeled compound or the free labeled compound in a gamma counter.
Claim 13
Dependent Claim
24 words
A method of radioimmunoassay for determining pterins as in claim 10 wherein the anti-pterines antibody is prepared by using 2-(5-carboxypentylamino)-4-hydroxy-6-(L-erythro-1,2-dihydroxypropyl)pteridine-BSA conjugate as an antigen.
Claim 14
Dependent Claim
18 words
A method of radioimmunoassay for determining pterines as in claim 10 wherein the labeled compound is radioiodinated 4-hydroxy-2-[2-(4-hydroxyphenyl)ethylamino]-6-(L-erythro-1,2-dihydroxypropyl)pteridine.
Claim 15
Dependent Claim
18 words
A method of radioimmunoassay for determining pterins as in claim 10 wherein the labeled compound is radioiodinated 2-[5-(tryaminocarbonyl)pentylamino]-4-hydroxy-6-(L-erythro-1,2-dihydroxypropyl)pteridine.
Claim 16
Dependent Claim
24 words
A method of radioimmunoassay for determining pterines as in claim 10 wherein the anti-pterines antibody is prepared by using 2-(5-carboxypentylamino)-4-hydroxy-6-(L-erythro-1,2,3-trihydroxylpropyl)pteridine-BSA conjugate as an antigen.
Claim 17
Dependent Claim
18 words
A method of radioimmunoassay for determining pterines as in claim 10 wherein the labeled compound is radioiodinated 2-[5-(tyraminocarbonyl)pentylamino]-4-hydroxy-6-(L-erythro-1,2,3-trihydroxypropyl)pteridine.
Claim 18
Dependent Claim
18 words
A method of radioimmunoassay for determining pterines as in claim 10 wherein the labeled compound is radioiodinated 4-hydroxy-2-[2-(4-hydroxyphenyl)ethylamino]-6-(L-erythro-1,2,3-trihydroxypropyl)pteridine.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-01-31 | 1986-08-02 | 1987-02-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-31 | 1990-08-02 | 1991-02-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-31 | 1994-08-02 | 1995-02-01 | $7700.00 | Window Closed / Lapsed |