Radioassay of folates

Claim 2

A chemical compound of the formula: ##STR3## wherein R is selected from the group of H, 125 I, and 131 I.

Claim 3

The compound of claim 1 in which R is H.

Claim 4

The compound in accordance with claim 1 wherein R is 125 I.

Claim 5

A tracer composition for radioassaying biological samples, comprising an iodinated conjugate of folic acid and histamine.

Claim 6

A tracer composition for radioassaying biological samples, comprising an iodinated conjugate of folic acid and histamine.

Claim 7

The composition of claim 4, further comprising an alkaline buffer agent.

Claim 8

A composition for use in radioassay analysis for folic acid, metabolites of folic acid, derivatives of folic acid, or mixtures thereof, comprising a folic acid-histamine conjugate.

Claim 9

A composition for use in radioassay analysis for folic acid, metabolites of folic acid, derivatives of folic acid, or mixtures thereof, comprising a folic acid-histamine conjugate.

Claim 10

The composition of claim 6, further comprising a radioactive tracer material attached to said conjugate.

Claim 11

The composition of claim 7, wherein the radioactive tracer material comprises an iodinated conjugate of folic acid and histamine.

Claim 12

The composition of claim 6, for use as a standard in said analysis, said standard consisting essentially of a folic acid-histamine conjugate.

Claim 15

The process of claim 10, wherein R is 125 I.

Claim 16

The process of claim 10, further comprising forming a second mixture of said labeled compound with a known amount of a standard, which is an unlabeled compound, in the presence of a folate binder to bind at least some of said labeled compound, separating the bound labeled compound from the remaining free labeled compound, measuring the radiation emitted from either the bound labeled compound or the free labeled compound from said second mixture by means of a gamma counter, and determining the amount of said folic acid compound by comparing the radioactivity of the bound or unbound labeled compound from said mixture with the radioactivity of the bound or unbound labeled compound, respectively, from said second mixture.

Claim 17

The method of claim 12, wherein said standard is an unlabeled compound having a chemical formula: ##STR5##

Claim 18

The method of claim 12, wherein said standard is unlabeled folic acid.

Claim 19

The method of claim 12, wherein said standard is unlabeled N,5-methyltetrahydrofolic acid.

Claim 20

An article of manufacture or mercantile unit in the form of a radioassay test kit suitable for analyzing biological liquids for folic acid, N,5-methyltetrahydrofolic acid, metabolites of folic acid, derivative of folic acid, and mixtures thereof, said unit comprising containers or similar articles which contain various chemical components to be used in the analysis, said components comprising a supply of a specific folate binder, a radioactive tracer having the chemical formula: ##STR6## wherein R is 125 I or 131 I, and a supply of standard compound, said containers being packaged together in said unit.

Claim 21

An article of manufacture or mercantile unit in the form of a radioassay test kit suitable for analyzing biological liquids for folic acid, N,5-methyltetrahydrofolic acid, metabolites of folic acid, derivative of folic acid, and mixtures thereof, said unit comprising containers or similar articles which contain various chemical components to be used in the analysis, said components comprising a supply of a specific folate binder, a radioactive tracer having the chemical formula: ##STR6## wherein R is 125 I or 131 I, and a supply of standard compound, said containers being packaged together in said unit.

Claim 22

The kit of claim 16, wherein the standard compound is a conjugate of folic acid and histamine.

Claim 23

The kit of claim 16, wherein the standard compound is folic acid.

Claim 24

The kit of claim 16, further comprising a charcoal suspension.

Claim 25

The kit of claim 16, further comprising a supply of buffering material.

Claim 26

The kit of claim 16, further comprising a supply of ascorbic acid.

Claim 29

The process of claim 22, further comprising forming a second mixture of said labeled compound with a known amount of a standard, which is an unlabeled compound, in the presence of a folate binder to bind at least some of said labeled compound, separating the bound labeled compound from the remaining free labeled compound, measuring the radiation emitted from either the bound labeled compound or the free labeled compound from said second mixture by means of a gamma counter, and determining the amount of said folic acid compound by comparing the radioactivity of the bound or unbound labeled compound from said mixture with the radioactivity of the bound or unbound labeled compound, respectively, from said second mixture.

Claim 30

The method of claim 23, wherein said standard is an unlabeled compound having a chemical formula: ##STR7##

Claim 31

The method of claim 23, wherein said standard is unlabeled folic acid.

Claim 32

The method of claim 23, wherein said standard is unlabeled N,5-methyltetrahydrofolic acid.

Claim 33

An article of manufacture or mercantile unit in the form of a radioassay test kit suitable for analyzing biological liquids for folic acid, N,5-methyltetrahydrofolic acid, metabolites of folic acid, derivative of folic acid, and mixtures thereof, said unit comprising containers or similar articles which contain various chemical components to be used in the analysis, said components comprising a supply of a specific folate binder, a radioactive tracer that is an iodinated conjugate of folic acid and histamine, and a supply of standard compound, said containers being packaged together in said unit.

Claim 34

An article of manufacture or mercantile unit in the form of a radioassay test kit suitable for analyzing biological liquids for folic acid, N,5-methyltetrahydrofolic acid, metabolites of folic acid, derivative of folic acid, and mixtures thereof, said unit comprising containers or similar articles which contain various chemical components to be used in the analysis, said components comprising a supply of a specific folate binder, a radioactive tracer that is an iodinated conjugate of folic acid and histamine, and a supply of standard compound, said containers being packaged together in said unit.

Claim 35

The kit of claim 27, wherein the standard compound is a conjugate of folic acid and histamine.

Claim 36

The kit of claim 27, wherein the standard compound is folic acid.

Claim 37

The kit of claim 27, further comprising a charcoal suspension.

Claim 38

The kit of claim 27, further comprising a supply of buffering material.

Claim 39

The kit of claim 27, further comprising a supply of ascorbic acid.