US4135880A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Folate assay with ion exchange resin bound-free separation
Assignee: Bio Rad Laboratories Inc
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Filing: 1977-08-19
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Granted: 1979-01-23
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Expires: 1997-08-19
Abstract
Abstract Improved competitive binding serum folate assay in which serum folate bound by folate-selective protein is separated from free serum folate by removal with an anion exchanger, preferably intermediate base anion exchange resin. Preferable are resins characterized by having an aliphatic lattice, particularly, a polyalkyleneamine lattice having tertiary and quaternary amine groups. The resin is advantageously added for the separation function in tablet form.
Inventors
- 👤 Vito J. Mangiardi
- 👤 Henry J. Jeong
- 👤 Nathan Lewin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 14:20
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/82; G01N30/00; G01N30/96; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
38 words
In a competitive binding assay for serum folate including the step of separating unbound serum folate from serum folate bound by a folate-selective protein, the improvement comprising separating unbound serum folate by removal with an anion exchange resin.
Claim 2
Dependent Claim
38 words
In a competitive binding assay for serum folate including the step of separating unbound serum folate from serum folate bound by a folate-selective protein, the improvement comprising separating unbound serum folate by removal with an anion exchange resin.
Claim 3
Dependent Claim
22 words
The improved competitive binding assay in accordance with claim 1, wherein said anion exchange resin is an intermediate base anion exchange resin.
Claim 4
Dependent Claim
19 words
The improved competitive binding assay in accordance with claim 2, wherein said anion exchange resin has an aliphatic lattice.
Claim 5
Dependent Claim
19 words
The improved competitive binding assay in accordance with claim 1, wherein said anion exchange resin has an aromatic lattice.
Claim 6
Dependent Claim
24 words
The improved competitive binding assay in accordance with claim 3, wherein said anion exchange resin comprises tertiary and quarternary amines on a polyalkyleneamine lattice.
Claim 7
Dependent Claim
49 words
The improved competitive binding assay in accordance with claim 5, wherein the active groups in the resin include R - N - (CH 3 ) 2 Cl + and R × N - (CH 3 ) 2 (C 2 H 4 OH)Cl - , and R = polyalkyleneamine lattice.
Claim 8
Dependent Claim
86 words
An assay for serum folate comprising: providing a serum sample solution, treating said solution to free serum folate from serum folate binding proteins, inactivating the latter, competitively binding a portion of said serum folate with a folate-selective protein whereby said solution contains bound and free serum folate, adding about 3-140 mg for each 0.1 ml in the test solution of intermediate base anion exchange resin in dry solid form to said solution to adsorb free serum folate, and separating said anion exchange resin from said solution.
Claim 9
Dependent Claim
86 words
An assay for serum folate comprising: providing a serum sample solution, treating said solution to free serum folate from serum folate binding proteins, inactivating the latter, competitively binding a portion of said serum folate with a folate-selective protein whereby said solution contains bound and free serum folate, adding about 3-140 mg for each 0.1 ml in the test solution of intermediate base anion exchange resin in dry solid form to said solution to adsorb free serum folate, and separating said anion exchange resin from said solution.
Claim 10
Dependent Claim
23 words
The assay in accordance with claim 7, wherein said anion exchange resin is in a compressed tablet form when added to said solution.
Claim 11
Dependent Claim
27 words
The assay in accordance with claim 7, wherein said resin is added to constitute about 3-140 mg for each 0.1 ml of serum in a test solution.
Claim 12
Dependent Claim
27 words
The assay in accordance with claim 7, wherein said resin is added to constitute about 8-67 mg for each 0.1 ml of serum in a test solution.
Claim 13
Dependent Claim
17 words
The assay in accordance with claim 7, wherein said anion exchange resin is about 50-400 wet mesh.
Claim 14
Dependent Claim
17 words
The assay in accordance with claim 11, wherein said anion exchange resin is about 200-400 wet mesh.
References Cited (Backward Citations)
US4028465A
Cited
US4015939A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-01-22 | 1982-07-24 | 1983-01-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-01-22 | 1986-07-24 | 1987-01-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-01-22 | 1990-07-24 | 1991-01-23 | $7700.00 | Window Closed / Lapsed |