Patent US4369250A successfully refreshed from Google Patents.
US4369250A
Expired / Lapsed
US
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Fatty acid determination
Assignee: Covidien AG
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Filing: 1981-07-31
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Granted: 1983-01-18
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Expires: 2001-07-31
Abstract
Abstract A complex of a long chain cationic surfactant and an organic anionic dye is useful in demonstrating the presence of and determining the concentration of fatty acids, compounds which hydrolyze to liberate fatty acids, or the activity of hydrolaze enzymes which produce fatty acids from a substrate.
Inventors
- 👤 E. Melvin Gindler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 07:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; C; C12; C12Q; C12Q1/00; C12Q1/34; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/916; G01N2333/918; G01N2800/00; G01N2800/04; G01N2800/044; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10T; Y10T436/00; Y10T436/10; Y10T436/104165
Claims Analysis
Total Claims: 52
Claim 1
Independent Claim
70 words
A method of demonstrating the presence of a free fatty acid in a liquid sample, said method comprising the steps of: (a) mixing said sample with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; and, (b) observing the resulting color change in said sample.
Claim 2
Independent Claim
70 words
A method of demonstrating the presence of a free fatty acid in a liquid sample, said method comprising the steps of: (a) mixing said sample with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; and, (b) observing the resulting color change in said sample.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein said surfactant is a quaternary ammonium cationic surfactant.
Claim 4
Dependent Claim
33 words
The method of claim 2 wherein said surfactant is selected from the group consisting of surfactants having the following structures: ##STR4## wherein n is an integer between about 6 and 8, inclusive; ##STR5##
Claim 5
Dependent Claim
37 words
The method of claim 2 wherein said dye is selected from the group consisting of azo dyes, sulfonphthalein dyes, triphenylmethane dyes, fluorescein dyes, naphthalenesulfonic acid dyes, anthraquinone dyes, phenoxazine dyes, tricarbocyanine dyes, indigo dyes and acridine dyes.
Claim 6
Dependent Claim
41 words
The method of claim 4 wherein said surfactant comprises ##STR6## wherein n is an integer between about 6 and 8, inclusive, said dye is a sulfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
Claim 7
Dependent Claim
24 words
The method of claim 5 wherein said reaction is carried out at a pH of between about 8.5-8.8 in the presence of a buffer.
Claim 8
Independent Claim
81 words
A method of determining the concentration of a free fatty acid in a liquid sample, said method comprising the steps of: (a) mixing said sample with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acid reacts with said complex to liberate said dye from said complex; (b) observing the resulting color change in said sample; and, (c) comparing the degree of said color change to calibration means.
Claim 9
Independent Claim
81 words
A method of determining the concentration of a free fatty acid in a liquid sample, said method comprising the steps of: (a) mixing said sample with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acid reacts with said complex to liberate said dye from said complex; (b) observing the resulting color change in said sample; and, (c) comparing the degree of said color change to calibration means.
Claim 10
Dependent Claim
14 words
The method of claim 7 wherein said surfactant is a quaternary ammonium cationic surfactant.
Claim 11
Dependent Claim
33 words
The method of claim 8 wherein said surfactant is selected from the group consisting of surfactants having the following structures: ##STR7## wherein n is an integer between about 6 and 8, inclusive; ##STR8##
Claim 12
Dependent Claim
37 words
The method of claim 8 wherein said dye is selected from the group consisting of azo dyes, sulfonphthalein dyes, triphenylmethane dyes, fluorescein dyes, naphthalenesulfonic acid dyes, anthraquinone dyes, phenoxazine dyes, tricarbocyanine dyes, indigo dyes and acridine dyes.
Claim 13
Dependent Claim
41 words
The method of claim 10 wherein said surfactant comprises ##STR9## wherein in is an integer between about 6 and 8, inclusive, said dye is a solfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
Claim 14
Dependent Claim
24 words
The method of claim 11 wherein said reaction is carried out at a pH of between about 8.5-8.8 in the presence of a buffer.
Claim 15
Independent Claim
94 words
A method of demonstrating the presence in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component to liberate said free fatty acids to produce a first reaction mixture; (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; and, (c) observing the resulting color change in said sample.
Claim 16
Independent Claim
94 words
A method of demonstrating the presence in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component to liberate said free fatty acids to produce a first reaction mixture; (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; and, (c) observing the resulting color change in said sample.
Claim 17
Dependent Claim
14 words
The method of claim 13 wherein said surfactant is a quaternary ammonium cationic surfactant.
Claim 18
Dependent Claim
33 words
The method of claim 14 wherein said surfactant is selected from the group consisting of surfactants having the following structures: ##STR10## wherein n is an integer between about 6 and 8, inclusive; ##STR11##
Claim 19
Dependent Claim
37 words
The method of claim 14 wherein said dye is selected from the group consisting of azo dyes, sulfonphthalein dyes, triphenylmethane dyes, fluorescein dyes, naphthalenesulfonic acid dyes, anthraquinone dyes, phenoxazine dyes, tricarbocyanine dyes, indigo dyes and acridine dyes.
Claim 20
Dependent Claim
41 words
The method of claim 16 wherein said surfactant comprises ##STR12## wherein n is an integer between about 6 and 8, inclusive, said dye is a sulfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
Claim 21
Dependent Claim
24 words
The method of claim 17 wherein said reaction is carried out at a pH of between about 8.5-8.8 in the presence of a buffer.
Claim 22
Independent Claim
98 words
A method of demonstrating the presence in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component with a hydrolase enzyme to liberate said free fatty acids to produce a first reaction mixture; (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; and, (c) observing the resulting color change in said sample.
Claim 23
Independent Claim
98 words
A method of demonstrating the presence in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component with a hydrolase enzyme to liberate said free fatty acids to produce a first reaction mixture; (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; and, (c) observing the resulting color change in said sample.
Claim 24
Independent Claim
106 words
A method of determining the concentration in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component to liberate said free fatty acids to produce a first reaction mixture; and, (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; (c) observing the resulting color change in said sample; and, (d) comparing the degree of said color change to calibration means.
Claim 25
Independent Claim
106 words
A method of determining the concentration in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component to liberate said free fatty acids to produce a first reaction mixture; and, (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; (c) observing the resulting color change in said sample; and, (d) comparing the degree of said color change to calibration means.
Claim 26
Dependent Claim
14 words
The method of claim 20 wherein said surfactant is a quaternary ammonium cationic surfactant.
Claim 27
Dependent Claim
33 words
The method of claim 21 wherein said surfactant is selected from the group consisting of surfactants having the following structures: ##STR13## wherein n is an integer between about 6 and 8, inclusive; ##STR14##
Claim 28
Dependent Claim
37 words
The method of claim 1 wherein said dye is selected from the group consisting of azo dyes, sulfonphthalein dyes, triphenylmethane dyes, fluorescein dyes, naphthalenesulfonic acid dyes, anthraquinone dyes, phenoxazine dyes, tricarbocyanine dyes, indigo dyes and acridine dyes.
Claim 29
Dependent Claim
41 words
The method of claim 23 wherein said surfactant comprises ##STR15## wherein n is an integer between about 6 and 8, inclusive, said dye is a sulfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
Claim 30
Dependent Claim
24 words
The method of claim 24 wherein said reaction is carried out at a pH of between about 8.5-8.8 in the presence of a buffer.
Claim 31
Independent Claim
109 words
A method of determining the concentration in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component with a hydrolase enzyme to liberate said free fatty acids to produce a first reaction mixture; (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; (c) observing the resulting color change in said sample; and, (d) comparing the degree of said color change to calibration means.
Claim 32
Independent Claim
109 words
A method of determining the concentration in a liquid sample of a component which is hydrolyzable to liberate free fatty acids, said method comprising the steps of: (a) hydrolyzing said component with a hydrolase enzyme to liberate said free fatty acids to produce a first reaction mixture; (b) mixing said first reaction mixture with a reagent comprising a solution of a complex of a long chain cationic surfactant and an anionic dye whereby said free fatty acids react with said complex to liberate said dye from said complex; (c) observing the resulting color change in said sample; and, (d) comparing the degree of said color change to calibration means.
Claim 33
Independent Claim
88 words
A method of demonstrating the presence of a hydrolase enzyme in a liquid sample, said method comprising the steps of: (a) mixing said sample with a known amount of a material which is hydrolyzable by said enzyme to liberate fatty acids, and a reagent comprising a complex of a long chain cationic surfactant and an anionic dye whereby said material is hydrolyzed to liberate fatty acids, and said fatty acids react with said complex to liberate said dye; and, (b) observing the resulting color change in said sample.
Claim 34
Independent Claim
88 words
A method of demonstrating the presence of a hydrolase enzyme in a liquid sample, said method comprising the steps of: (a) mixing said sample with a known amount of a material which is hydrolyzable by said enzyme to liberate fatty acids, and a reagent comprising a complex of a long chain cationic surfactant and an anionic dye whereby said material is hydrolyzed to liberate fatty acids, and said fatty acids react with said complex to liberate said dye; and, (b) observing the resulting color change in said sample.
Claim 35
Dependent Claim
14 words
The method of claim 27 wherein said surfactant is a quaternary ammonium cationic surfactant.
Claim 36
Dependent Claim
33 words
The method of claim 28 wherein said surfactant is selected from the group consisting of surfactants having the following structures: ##STR16## wherein n is an integer between about 6 and 8, inclusive; ##STR17##
Claim 37
Dependent Claim
37 words
The method of claim 28 wherein said dye is selected from the group consisting of azo dyes, sulfonphthalein dyes, triphenylmethane dyes, fluorescein dyes, naphthalenesulfonic acid dyes, anthraquinone dyes, phenoxazine dyes, tricarbocyanine dyes, indigo dyes and acridine dyes.
Claim 38
Dependent Claim
41 words
The method of claim 30 wherein said surfactant comprises ##STR18## wherein n is an integer between about 6 and 8, inclusive, said dye is a sulfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
Claim 39
Dependent Claim
24 words
The method of claim 31 wherein said reaction is carried out at a pH of between about 8.5-8.8 in the presence of a buffer.
Claim 40
Independent Claim
95 words
A method of determining the concentration of hydrolase enzyme in a liquid sample, said method comprising the steps of: (a) mixing said sample with a known amount of a material which is hydrolyzable by said enzyme to liberate fatty acids, and a reagent comprising a complex of a long chain cationic surfactant and an anionic dye whereby said material is hydrolyzed to liberate fatty acids, and said fatty acids react with said complex to liberate said dye; (b) observing the resulting color change in said sample; and, (c) comparing said color change with calibration means.
Claim 41
Independent Claim
95 words
A method of determining the concentration of hydrolase enzyme in a liquid sample, said method comprising the steps of: (a) mixing said sample with a known amount of a material which is hydrolyzable by said enzyme to liberate fatty acids, and a reagent comprising a complex of a long chain cationic surfactant and an anionic dye whereby said material is hydrolyzed to liberate fatty acids, and said fatty acids react with said complex to liberate said dye; (b) observing the resulting color change in said sample; and, (c) comparing said color change with calibration means.
Claim 42
Dependent Claim
14 words
The method of claim 33 wherein said surfactant is a quaternary ammonium cationic surfactant.
Claim 43
Dependent Claim
33 words
The method of claim 34 wherein said surfactant is selected from the group consisting of surfactants having the following structures: ##STR19## wherein n is an integer between about 6 to 8, inclusive; ##STR20##
Claim 44
Dependent Claim
37 words
The method of claim 34 wherein said dye is selected from the group consisting of azo dyes, sulfonphthalein dyes, triphenylmethane dyes, fluorescein dyes, naphthalenesulfonic acid dyes, anthraquinone dyes, phenoxazine dyes, tricarbocyanine dyes, indigo dyes and acridine dyes.
Claim 45
Dependent Claim
41 words
The method of claim 36 wherein said surfactant comprises ##STR21## wherein n is an integer between about 6 and 8, inclusive, said dye is a sulfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
Claim 46
Dependent Claim
24 words
The method of claim 37 wherein said reaction is carried out at a pH of between about 8.5-8.8 in the presence of a buffer.
Claim 47
Dependent Claim
57 words
A reagent kit for determining the concentration of a component hydrolyzable by a hydrolase enzyme to liberate fatty acids, said reagent kit comprising first and second containers, said first container containing a solution of said hydrolase enzyme, and said second container containing a solution of a complex of a long chain cationic surfactant and an anionic dye.
Claim 48
Dependent Claim
57 words
A reagent kit for determining the concentration of a component hydrolyzable by a hydrolase enzyme to liberate fatty acids, said reagent kit comprising first and second containers, said first container containing a solution of said hydrolase enzyme, and said second container containing a solution of a complex of a long chain cationic surfactant and an anionic dye.
Claim 49
Dependent Claim
42 words
The reagent kit of claim 39 wherein said surfactant comprises ##STR22## wherein n is an integer between about 6 and 8, inclusive, said dye is a sulfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
Claim 50
Dependent Claim
60 words
A reagent kit for determining the concentration of a hydrolase enzyme in a sample, said reagent kit comprising first and second containers, said first container containing a material hydrolyzable by said hydrolase enzyme to liberate fatty acids from said material, and said second container containing a solution of a complex of a long chain cationic surfactant and an anionic dye.
Claim 51
Dependent Claim
60 words
A reagent kit for determining the concentration of a hydrolase enzyme in a sample, said reagent kit comprising first and second containers, said first container containing a material hydrolyzable by said hydrolase enzyme to liberate fatty acids from said material, and said second container containing a solution of a complex of a long chain cationic surfactant and an anionic dye.
Claim 52
Dependent Claim
42 words
The reagent kit of claim 41 wherein said surfactant comprises ##STR23## wherein n is an integer between about 6 and 8, inclusive, said dye is a sulfobromphthalein dye, and said surfactant and dye are present in said complex in substantially equimolar amounts.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-01-17 | 1986-07-19 | 1987-01-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-17 | 1990-07-19 | 1991-01-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-17 | 1994-07-19 | 1995-01-18 | $7700.00 | Window Closed / Lapsed |