US3884637A
Expired / Lapsed
US
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Method and composition for the determination of albumin
Assignee: Pierce Chemical Co
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Filing: 1973-03-21
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Granted: 1975-05-20
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Expires: 1993-03-21
Abstract
Abstract A working solution and method for the spectrophotometric determination of albumin, employing a sulphonphthalein dye such as bromcresol green and a phosphate-containing anionic surfactant to prevent precipitation of protein-dye complexes.
Inventors
- 👤 E Melvin Gindler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6803; G01N33/6827; G01N33/6839
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
88 words
IN A METHOD FOR THE DETERMINATION OF ALBUMIN COMPRISING ADDING TO AN ALBUMIN CONTAINING BIOLOGIC FLUID AN AQUEOUS SOLUTION CONTANING A SULPHONPHTHALEIN DYE BUFFERED TO AN APPROPRIATE PH FOR SENSITIVE SPECTROPHOTOMETRIC ANALYSIS AND THEREAFTER SPECTROPHOTOMETRICALLY MEASURING THE ABSORBANCE OF THE SOLUTION SO FORMED; THE IMPROVEMENT WHEREIN THE AQUEOUS SOLUTION ADDED TO THE BIOLOGIC FLUID CONTAINS A WATER SOLUBLE, PHOSPHATE CONTAINING ANIONIC SURFACTANT FORMED FROM A HYDROPHOBIC ALCOHOL HAVING AT LEAST EIGHT CARBON ATOMS, SAID ANIONIC SURFACTANT BEING PRESENT IN AN AMOUNT SUFFICIENT TO PREVENT PRECIPITATION OF PROTEIN-DYE COMPLEXES.
Claim 2
Dependent Claim
88 words
IN A METHOD FOR THE DETERMINATION OF ALBUMIN COMPRISING ADDING TO AN ALBUMIN CONTAINING BIOLOGIC FLUID AN AQUEOUS SOLUTION CONTANING A SULPHONPHTHALEIN DYE BUFFERED TO AN APPROPRIATE PH FOR SENSITIVE SPECTROPHOTOMETRIC ANALYSIS AND THEREAFTER SPECTROPHOTOMETRICALLY MEASURING THE ABSORBANCE OF THE SOLUTION SO FORMED; THE IMPROVEMENT WHEREIN THE AQUEOUS SOLUTION ADDED TO THE BIOLOGIC FLUID CONTAINS A WATER SOLUBLE, PHOSPHATE CONTAINING ANIONIC SURFACTANT FORMED FROM A HYDROPHOBIC ALCOHOL HAVING AT LEAST EIGHT CARBON ATOMS, SAID ANIONIC SURFACTANT BEING PRESENT IN AN AMOUNT SUFFICIENT TO PREVENT PRECIPITATION OF PROTEIN-DYE COMPLEXES.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the sulphonphthalein dye is bromcresol green.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein the anionic surfactant has the following structure:
Claim 5
Dependent Claim
19 words
The method of claim 3 wherein n is 2-10 and R is an alkyl radical having 14-22 carbon atoms
Claim 6
Dependent Claim
25 words
The method of claim 4 wherein, based on the weight of the biologic fluid, the anionic surfactant is present in an amount of about 65-85%.
Claim 7
Dependent Claim
62 words
The method of claim 4 wherein the anionic surfactant is further characterized as a partial sodium salt of a mixture of mono and diphosphate esters having a specific gravity (25*C) of 1.05-1.15, a pour point (ASTM) of 6*C, an acid number (mg KOH/g product) of approximately 35 to the second inflection point (pH 9-9.5) and pH of 5-6 (10% solution at 25*C).
Claim 8
Dependent Claim
25 words
The method of claim 4 wherein, based on the weight of the biologic fluid, the anionic surfactant is present in an amount of about 40-300%.
Claim 9
Dependent Claim
63 words
The method of claim 7 wherein the anionic surfactant is further characterized as a partial sodium salt of a mixture of mono and diphosphate esters having a specific gravity (25*C) of 1.05-1.15, a pour point (ASTM) of 6*C, an acid number (mg KOH/g product) of approximately 35 to the second inflection point (pH 9-9.5) and a pH of 5-6 (10% solution at 25*C).
Claim 10
Dependent Claim
24 words
The method of claim 8 wherein the dye is present in an amount of about 0.5-5%, based on the weight of the biologic fluid.
Claim 11
Dependent Claim
37 words
An aqueous solution useful in the spectrophotometric determination of albumin which consists essentially of water, a sulphonphthalein dye, and a water soluble, phosphate containing anionic surfactant formed from a hydrophobic alcohol having at least eight carbon atoms.
Claim 12
Dependent Claim
37 words
An aqueous solution useful in the spectrophotometric determination of albumin which consists essentially of water, a sulphonphthalein dye, and a water soluble, phosphate containing anionic surfactant formed from a hydrophobic alcohol having at least eight carbon atoms.
Claim 13
Dependent Claim
9 words
The solution of claim 10 which contains a buffer.
Claim 14
Dependent Claim
11 words
The solution of claim 11 wherein the dye is bromcresol green.
Claim 15
Dependent Claim
65 words
The solution of claim 12 wherein the anionic surfactant is characterized as a partial sodium salt of a mixture of mono And diphosphate esters having a specific gravity (25* C.) of 1.05-1.15, a pour point (ASTM) of 6* C., an acid number (mg KOH/g product) of approximately 35 to the second inflection point (pH 9-9.5) and a pH of 5-6 (10% solution at 25* C).
References Cited (Backward Citations)
US3533749A
Cited
US3558278A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-05-19 | 1978-11-18 | 1979-05-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-05-19 | 1982-11-18 | 1983-05-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-05-19 | 1986-11-18 | 1987-05-20 | $7700.00 | Window Closed / Lapsed |