Patent US4350761A successfully refreshed from Google Patents.
US4350761A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of and reagents for quantitative analysis of cyclic nucleotides
Assignee: YAMASU SHOYU KK
•
Filing: 1980-05-15
•
Granted: 1982-09-21
•
Expires: 2000-05-15
Abstract
Abstract A method of quantitative analysis of cyclic nucleotides which comprises: acylating a cyclic nucleotide in an assay sample by using an acylating agent; causing said acyl-cyclic nucleotide and a specific quantity of an enzyme-labeled cyclic nucleotide formed by bonding a cyclic nucleotide corresponding to the acyl-cyclic nucleotide and an enzyme through a dicarboxylic acid to undergo competitive reaction with a specific quantity of an antibody with respect to a corresponding cyclic nucleotide; separating the enzyme-labeled cyclic nucleotide which has become bonded to said antibody and the enzyme-labeled cyclic nucleotide which has not become bonded thereto; and determining the quantity of the cyclic nucleotide by measuring the enzyme activity of either of the enzyme-labeled cyclic nucleotides. For this analysis, a reagent comprising an enzyme-labeled cyclic nucleotide is used.
Inventors
- 👤 Itaru Yamamoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 19:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5308; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/975; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
123 words
A method for the quantitative analysis of cyclic nucleotides which comprises: acylating a cyclic nucleotide in an assay sample by using an acylating agent; causing said acyl-cyclic nucleotide and a predetermined quantity of an enzyme-labeled cyclic nucleotide formed by bonding a cyclic nucleotide corresponding to the cyclic nucleotide to be assayed and an enzyme through a dicarboxylic acid to undergo a competitive antigen-antibody reaction with a predetermined quantity of an antibody corresponding to the cyclic nucleotide to be assayed; separating the enzyme-labeled cyclic nucleotide which has become bonded to said antibody and the enzyme-labeled cyclic nucleotide which has not become bonded thereto; and measuring the enzyme activity of either of said enzyme-labeled cyclic nucleotides thereby to determine the quantity of the cyclic nucleotide.
Claim 2
Independent Claim
123 words
A method for the quantitative analysis of cyclic nucleotides which comprises: acylating a cyclic nucleotide in an assay sample by using an acylating agent; causing said acyl-cyclic nucleotide and a predetermined quantity of an enzyme-labeled cyclic nucleotide formed by bonding a cyclic nucleotide corresponding to the cyclic nucleotide to be assayed and an enzyme through a dicarboxylic acid to undergo a competitive antigen-antibody reaction with a predetermined quantity of an antibody corresponding to the cyclic nucleotide to be assayed; separating the enzyme-labeled cyclic nucleotide which has become bonded to said antibody and the enzyme-labeled cyclic nucleotide which has not become bonded thereto; and measuring the enzyme activity of either of said enzyme-labeled cyclic nucleotides thereby to determine the quantity of the cyclic nucleotide.
Claim 3
Dependent Claim
30 words
The method as claimed in claim 1 in which the cyclic nucleotide is selected from the group consisting of adenosine-3',5'-cyclic monophosphate, guanosine-3',5'-cyclic monophosphate, inosine-3',5'-cyclic monophosphate, cytidine-3',5'-cyclic monophosphate, and uridine-3',5'-cyclic monophosphate.
Claim 4
Dependent Claim
48 words
The method as claimed in claim 1 in which the enzyme in the enzyme-labeled cyclic nucleotide is selected from the group consisting of β-D-galactosidase, peroxidase, alkaline phosphatase, glucose oxidase, glucoamylase, lysozyme, malate dehydrogenase, glucose-6-phosphate dehydrogenase, acetylcholine esterase, lipase, β-glucuronidase, hyaluronidase, β-glucosidase, and an active fragment of said enzyme.
Claim 5
Dependent Claim
24 words
The method as claimed in claim 1 in which the acylating agent is a combination of an acid anhydride and an organic tertiary amine.
Claim 6
Dependent Claim
26 words
The method as claimed in claim 4 in which the organic tertiary amine is selected from the group consisting of aliphatic tertiary amines and heterocyclic bases.
Claim 7
Dependent Claim
29 words
The method as claimed in claim 1 in which the antibody is selected from the group consisting of an antibody immobilized on an insoluble carrier and a soluble antibody.
Claim 8
Dependent Claim
44 words
The method as claimed in claim 6 in which the insoluble carrier is selected from the group consisting of silicone, glass, ceramics, polystyrenes, crosslinked polyacrylamides, plastics, CM cellulose, DEAE cellulose, cellulose, cross-linked dextran, filter paper and a carrier coated with an organic silane derivative.
Claim 9
Dependent Claim
45 words
A method according to claim 1 in which the cyclic nucleotide and acylating agent are reacted at room temperature for a period of 5 to 60 minutes and the antigen-antibody reactor proceeds at 1° to 5° C. for a period of 6 to 48 hours.
Claim 10
Dependent Claim
94 words
A kit for the quantitative analysis of cyclic nucleotides comprising, in combination, the following components each being in individual tubes: (a) an enzyme-labeled cyclic nucleotide; (b) an antibody to a cyclic nucleotide immobilized on an insoluble carrier; (c) an acid anhydride; (d) an organic tertiary amine; (e) a buffer solution; (f) a cyclic nucleotide standard solution; (g) an enzyme substrate; and (h) a reaction terminating agent; said components (a) through (h) being present in amounts sufficient to perform the quantitative analysis of cyclic nucleotides according to the methods of any one of claims 1-7.
Claim 11
Dependent Claim
98 words
A kit for the quantitative analysis of cyclic nucleotides comprising, in combination, the following components each being in individual tubes: (a) an enzyme-labeled cyclic nucleotide; (b) an antibody to a cyclic nucleotide immobilized on an insoluble carrier; (c) an acid anhydride; (d) an organic tertiary amine; (e) a buffer solution; (f) a cyclic nucleotide standard solution; (g) an enzyme substrate; (h) a reaction terminating agent; and (i) a phosphodiesterase inhibitor; said components (a) through (i) being present in amounts sufficient to perform the quantitative analysis of cyclic nucleotides according to the methods of any one of claims 1-7.
Claim 12
Dependent Claim
93 words
A kit for the quantitative analysis of cyclic nucleotides comprising, in combination, the following components each being in individual tubes: (a) an enzyme-labeled cyclic nucleotide; (b) an antibody to a cyclic nucleotide; (c) a separation agent; (d) an acid anhydride; (e) an organic tertiary amine; (f) a buffer solution; (g) a cyclic nucleotide standard solution; (h) an enzyme substrate; and (i) a reaction terminating agent; said components (a) through (i) being present in amounts sufficient to perform the quantitative analysis of cyclic nucleotides according to the methods of any one of claims 1-7.
Claim 13
Dependent Claim
97 words
A kit for the quantitative analysis of cyclic nucleotides comprising, in combination, the following components each being in individual tubes: (a) an enzyme-labeled cyclic nucleotide; (b) an antibody to a cyclic nucleotide; (c) a separation agent; (d) an acid anhydride; (e) an organic tertiary amine; (f) a buffer solution; (g) a cyclic nucleotide standard solution; (h) an enzyme substrate; (i) a reaction terminating agent; and (j) a phosphodiesterase inhibitor; said components (a) through (j) being present in amounts sufficient to perform the quantitative analysis of cyclic nucleotides according to the methods of any one of claims 1-7.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-09-20 | 1986-03-22 | 1986-09-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-09-20 | 1990-03-22 | 1990-09-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-09-20 | 1994-03-22 | 1994-09-21 | $7700.00 | Window Closed / Lapsed |