Patent US4346170A successfully refreshed from Google Patents.
US4346170A
Expired / Lapsed
US
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Method for producing L-lysine by fermentation
Assignee: Ajinomoto Co Inc
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Filing: 1980-07-23
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Granted: 1982-08-24
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Expires: 2000-07-23
Abstract
Abstract An L-lysine producing microorganism which is obtained by incorporation into a host strain of the genus Escherichia of a hybrid plasmid having inserted therein a DNA fragment with genetic information controlling L-lysine production which is derived from a donor strain which is resistant to an L-lysine analogue, is useful for the production of high levels of L-lysine by fermentation.
Inventors
- 👤 Kounosuke Sano
- 👤 Takayasu Tsuchida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 21:31
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12P; C12P13/00; C12P13/04; C12P13/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
52 words
An L-lysine producing organism which is obtained by incorporation into a host strain of the genus Escherichia of a hybrid vector having inserted therein a DNA fragment with genetic information controlling L-lysine production, which fragment is derived from a donor strain of the genus Escherichia which is resistant to an L-lysine analogue.
Claim 2
Dependent Claim
52 words
An L-lysine producing organism which is obtained by incorporation into a host strain of the genus Escherichia of a hybrid vector having inserted therein a DNA fragment with genetic information controlling L-lysine production, which fragment is derived from a donor strain of the genus Escherichia which is resistant to an L-lysine analogue.
Claim 3
Dependent Claim
15 words
The microorganism of claim 1, wherein said host strain is Escherichia coli BT-14, NRRL B-12200.
Claim 4
Dependent Claim
15 words
The microorganism of claim 1, wherein said donor strain is Escherichia coli EL-1, NRRL B-12199.
Claim 5
Dependent Claim
26 words
The microorganism of claim 1, wherein said donor strain is resistant to an analogue selected from the group consisting of oxo-lysine, lysine-hydroxamate, S-(2-aminoethyl)-cysteine, γ-methyl-lysine, and β-chloro-caprolactam.
Claim 6
Dependent Claim
11 words
The microorganism of claim 1, which is Eschericha coli NRRL B-12185.
Claim 7
Dependent Claim
34 words
The microorganism of claim 1, wherein said hybrid vector is derived from a member selected from the group consisting of Col E1, pSC 101, pBR 322, pACYC 177, pCR 1, R6K and γ phage.
Claim 8
Dependent Claim
13 words
The microorganism of claim 1, wherein said vector is derived from Col E1.
Claim 9
Dependent Claim
61 words
A hybrid vector derived from insertion into a vector selected from the group consisting of Col E1, pSC 101, pBR 322, pACYC 177, pCR 1, R6K and γ phage, of a DNA fragment containing information controlling L-lysine production, which DNA fragment is derived from the DNA of a donor strain of the genus Escherichia which is resistant to an L-lysine analogue.
Claim 10
Dependent Claim
61 words
A hybrid vector derived from insertion into a vector selected from the group consisting of Col E1, pSC 101, pBR 322, pACYC 177, pCR 1, R6K and γ phage, of a DNA fragment containing information controlling L-lysine production, which DNA fragment is derived from the DNA of a donor strain of the genus Escherichia which is resistant to an L-lysine analogue.
Claim 11
Dependent Claim
11 words
The vector of claim 8, which is derived from Col E1.
Claim 12
Dependent Claim
15 words
The vector of claim 8, wherein said donor strain is Escherichia coli EL-1, NRRL 12199.
Claim 13
Independent Claim
73 words
A method of producing L-lysine which comprises culturing in a culture medium an L-lysine producing microorganism which is obtained by incorporation into a host strain of the genus Escherichia, of a hybrid vector having inserted therein a DNA fragment with genetic information controlling L-lysine production which fragment is derived from a donor strain of the genus Escherichia which is resistant to an L-lysine analogue, and recovering the L-lysine accumulated in the culture medium.
Claim 14
Independent Claim
73 words
A method of producing L-lysine which comprises culturing in a culture medium an L-lysine producing microorganism which is obtained by incorporation into a host strain of the genus Escherichia, of a hybrid vector having inserted therein a DNA fragment with genetic information controlling L-lysine production which fragment is derived from a donor strain of the genus Escherichia which is resistant to an L-lysine analogue, and recovering the L-lysine accumulated in the culture medium.
Claim 15
Dependent Claim
15 words
The method of claim 11, wherein said host strain is Escherichia coli BT-14, NRRL B-12200.
Claim 16
Dependent Claim
15 words
The method of claim 11, wherein said donor strain is Escherichia coli EL-1, NRRL B-12199.
Claim 17
Dependent Claim
26 words
The method of claim 11, wherein said donor strain is resistant to an analogue selected from the group consisting of oxo-lysine, lysine-hydroxamate, S-(2-aminoethyl)-cysteine, γ-methy-lysine and β-chloro-caprolactam.
Claim 18
Dependent Claim
15 words
The method of claim 11, wherein said L-lysine producing microorganism is Escherichia coli NRRL B-12185.
Claim 19
Dependent Claim
34 words
The method of claim 11, wherein said hybrid vector is derived from a member selected from the group consisting of Col E1, pSC 101, pBR 322, pACYC 177, pCR 1, R6K and λ phage.
Claim 20
Dependent Claim
13 words
The method of claim 11, wherein said vector is derived from Col E1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-08-23 | 1986-02-22 | 1986-08-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-08-23 | 1990-02-22 | 1990-08-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-08-23 | 1994-02-22 | 1994-08-24 | $7700.00 | Window Closed / Lapsed |