US3905866A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for production of L-lysine by fermentation
Assignee: Kanegafuchi Chemical Industry Co Ltd
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Filing: 1973-08-02
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Granted: 1975-09-16
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Expires: 1993-08-02
Abstract
Abstract A process for the production of L-lysine by fermentation which comprises cultivating aerobically an L-lysine-producing mutant strain of a microorganism belonging to the Genus Pseudomonas or Achromobacter in a culture medium containing a hydrocarbon as a main carbon source until a substantial amount of L-lysine is accumulated in the culture medium and recovering the thus accumulated L-lysine from the culture broth is disclosed. The mutant strain used in the present invention has a resistance to at least one amino acid or analogue thereof selected from the group consisting of L-valine, Alpha -aminobutyric acid, norvaline, +-hydroxynorvaline, Alpha -amino- Beta -chlorobutyric acid and L-threonine.
Inventors
- 👤 Kiyoshi Watanabe
- 👤 Tutomu Tanaka
- 👤 Tamotsu Hirakawa
- 👤 Hideaki Kinoshita
- 👤 Mamoru Sasaki
- 👤 Koji Obayashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 07:02
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/824; Y10S435/874
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
100 words
A PROCESS FOR THE PRODUCTION OF L-LYSINE BY FERMENTATION WHICH COMPRISES CULTIVATING AEROBICALLY AN L-LYSINE-PRODUCING MUTANT STRAIN OF A MICROORGANISM BELONGING TO THE GENUS PSEUDOMONAS OR BELONGING TO ACHROMOBACTER COAGULANS ATCC 21934 WHICH ARE THE PARENT STRAINS, IN A CULTURE MEDIUM CONTAINING A HYDROCARBON AS A MAIN CARBON SOURCE UNTIL A SUBSTANTIAL AMOUNT OF L-LYSINE IS ACCUMULATED IN THE CULTURE MEDIUM AND RECOVERING THE THUS ACCUMULATED L-LYSINE FROM THE CULTURE BROTH, SAID MUTANT STRAIN BEING RESISTANT TO AT LEAST ONE AMINO ACID OR ANALOGUE THEREOF SELECTED FROM THE GROUP CONSISTING OF L-VALINE A-AMINO-BUTYRIC ACID, NORVALINE, BHYDROXYNORVALINE, A-AMINO-B-CHLOROBUTYRIC ACID AND L-THREONINE.
Claim 2
Dependent Claim
100 words
A PROCESS FOR THE PRODUCTION OF L-LYSINE BY FERMENTATION WHICH COMPRISES CULTIVATING AEROBICALLY AN L-LYSINE-PRODUCING MUTANT STRAIN OF A MICROORGANISM BELONGING TO THE GENUS PSEUDOMONAS OR BELONGING TO ACHROMOBACTER COAGULANS ATCC 21934 WHICH ARE THE PARENT STRAINS, IN A CULTURE MEDIUM CONTAINING A HYDROCARBON AS A MAIN CARBON SOURCE UNTIL A SUBSTANTIAL AMOUNT OF L-LYSINE IS ACCUMULATED IN THE CULTURE MEDIUM AND RECOVERING THE THUS ACCUMULATED L-LYSINE FROM THE CULTURE BROTH, SAID MUTANT STRAIN BEING RESISTANT TO AT LEAST ONE AMINO ACID OR ANALOGUE THEREOF SELECTED FROM THE GROUP CONSISTING OF L-VALINE A-AMINO-BUTYRIC ACID, NORVALINE, BHYDROXYNORVALINE, A-AMINO-B-CHLOROBUTYRIC ACID AND L-THREONINE.
Claim 3
Dependent Claim
18 words
A process according to claim 1 wherein said microorganism is a mutant strain of Pseudomonas brevis ATCC 21940.
Claim 4
Dependent Claim
15 words
A process according to claim 1 wherein said mutant strain is Pseudomonas brevis ATCC 21941.
Claim 5
Dependent Claim
18 words
A process according to claim 1 wherein said mutant strain is Achromobacter coagulans ATCC 21935 or ATCC 21936.
Claim 6
Dependent Claim
19 words
A process according to claim 1 wherein said hydrocarbon is kerosene or n-paraffin having 10 to 20 carbon atoms.
References Cited (Backward Citations)
US3759789A
Cited
US3756916A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-15 | 1979-03-17 | 1979-09-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-15 | 1983-03-17 | 1983-09-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-15 | 1987-03-17 | 1987-09-16 | $7700.00 | Window Closed / Lapsed |