US3871960A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing l-lysine by fermentation
Assignee: Ajinomoto Co Inc
•
Filing: 1973-09-25
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Granted: 1975-03-18
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Expires: 1993-09-25
Abstract
Abstract Strains of the genus Corynebacterium which combine resistance to feedback inhibition by S-(2-aminoethyl)-L-cysteine with methionine sensitivity and additionally may require proline or arginine, produce a large amount of L-lysine by fermentation of otherwise conventional culture media.
Inventors
- 👤 Koji Kubota
- 👤 Yasuhiko Yoshihara
- 👤 Hiroshi Okada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 20:22
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/843
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
11 words
SAID STRAIN BEING RESISTANT TO FEEDBACK INHIBITION BY 1 MG/ML S-(2-AMINOETHYL)-L-CYSTEINE,
Claim 2
Dependent Claim
11 words
SAID STRAIN BEING RESISTANT TO FEEDBACK INHIBITION BY 1 MG/ML S-(2-AMINOETHYL)-L-CYSTEINE,
Claim 3
Independent Claim
60 words
A METHOD OF PRODUCING L-LYSINE WHICH COMPRISES: A. CULTURING A LYSINE-PRODUCING STRAIN OF CORYNEBACTERIUM UNDER AEROBIC CONDITIONS IN AN AQUEOUS MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN, INORGANIC SALTS AND ORGANIC SUBSTANCES REQUIRED FOR THE GROWTH OF SAID STRAIN AT PH 5 TO 9 UNTIL L-LYSINE ACCUMULATES IN SAOD MEDIUM, AND B. RECOVERING THE ACCUMULATED LYSINE FROM SAID MEDIUM,
Claim 4
Independent Claim
60 words
A METHOD OF PRODUCING L-LYSINE WHICH COMPRISES: A. CULTURING A LYSINE-PRODUCING STRAIN OF CORYNEBACTERIUM UNDER AEROBIC CONDITIONS IN AN AQUEOUS MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN, INORGANIC SALTS AND ORGANIC SUBSTANCES REQUIRED FOR THE GROWTH OF SAID STRAIN AT PH 5 TO 9 UNTIL L-LYSINE ACCUMULATES IN SAOD MEDIUM, AND B. RECOVERING THE ACCUMULATED LYSINE FROM SAID MEDIUM,
Claim 5
Dependent Claim
29 words
the growth of said strain being severely inhibited during 24 hours cultivation on a minimal medium when said minimal medium is supplemented with 0.1 millimole per liter methionine, and
Claim 6
Dependent Claim
29 words
the growth of said strain being severely inhibited during 24 hours cultivation on a minimal medium when said minimal medium is supplemented with 0.1 millimole per liter methionine, and
Claim 7
Dependent Claim
27 words
A method as set forth in claim 1, wherein said strain further requires at least one amino acid selected from the group consisting of proline and arginine.
Claim 8
Dependent Claim
29 words
THE GROWTH OF SAID STRAIN BEING SEVERELY INHIBITED DURING 24 HOURS CULTIVATION ON A MINIMAL MEDIUM WHEN SAID MINIMAL MEDIUM IS SUPPLEMENTED WITH 0.1 MILLIMOLE PER LITER METHIONINE, AND
Claim 9
Dependent Claim
29 words
THE GROWTH OF SAID STRAIN BEING SEVERELY INHIBITED DURING 24 HOURS CULTIVATION ON A MINIMAL MEDIUM WHEN SAID MINIMAL MEDIUM IS SUPPLEMENTED WITH 0.1 MILLIMOLE PER LITER METHIONINE, AND
Claim 10
Dependent Claim
24 words
the inhibition of said growth by said methionine not being overcome by addition of L-threonine in any amount up to 100 millimole per liter.
Claim 11
Dependent Claim
24 words
the inhibition of said growth by said methionine not being overcome by addition of L-threonine in any amount up to 100 millimole per liter.
Claim 12
Dependent Claim
18 words
A method as set forth in claim 1, wherein said strain is a mutant strain of Corynebacterium glutamicum.
Claim 13
Dependent Claim
24 words
THE IHIBITION OF SAID GROWTH BY SAID METHIONIE NOT BEING OVERCOME BY ADDITION OF L-THREONINE IN ANY AMOUNT UP TO 100 MILLIMOLE PER LITER.
Claim 14
Dependent Claim
24 words
THE IHIBITION OF SAID GROWTH BY SAID METHIONIE NOT BEING OVERCOME BY ADDITION OF L-THREONINE IN ANY AMOUNT UP TO 100 MILLIMOLE PER LITER.
Claim 15
Independent Claim
65 words
A method of producing L-lysine which comprises culturing Corynebacterium glutamicum FERM P1638, FERM P 2278, or FERM P 2279 under aerobic condition in an aqueous medium containing aSsimilable sources of carbon and nitrogen, inorganic salts and organic substances required for the growth of said Corynebacterium glutamicum at pH 5 to 9 until L-lysine accumulates in said medium; and recovering the accumulated lysine from said medium.
Claim 16
Independent Claim
65 words
A method of producing L-lysine which comprises culturing Corynebacterium glutamicum FERM P1638, FERM P 2278, or FERM P 2279 under aerobic condition in an aqueous medium containing aSsimilable sources of carbon and nitrogen, inorganic salts and organic substances required for the growth of said Corynebacterium glutamicum at pH 5 to 9 until L-lysine accumulates in said medium; and recovering the accumulated lysine from said medium.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-17 | 1978-09-16 | 1979-03-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-17 | 1982-09-16 | 1983-03-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-17 | 1986-09-16 | 1987-03-18 | $7700.00 | Window Closed / Lapsed |