Patent US4273867A successfully refreshed from Google Patents.
US4273867A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and reagent for counteracting lipemic interference
Assignee: Takeda GmbH
•
Filing: 1979-04-05
•
Granted: 1981-06-16
•
Expires: 1999-04-05
Abstract
Abstract A method and reagent for counteracting lipemic interference in an assay of a lipid-containing sample, e.g. blood. The method includes the steps of: (a) mixing with the sample prior to or during said assay a high surface area, finely-divided adsorbent having an affinity for lipids, and a nonionic, water-soluble polymer, and (b) precipitating adsorbed lipids in said sample. The reagent includes a high surface area finely-divided adsorbent, and a nonionic, water-soluble polymer having a molecular weight of from about 1,000 to 100,000.
Inventors
- 👤 Wayne H. T. Lin
- 👤 James J. Grib
- 👤 Larry D. Mosier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:21
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; G01N2800/00; G01N2800/04; G01N2800/044; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S435/968; Y10S436/00; Y10S436/804; Y10S436/825
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
122 words
In a double antibody immunoassay for a substance in a lipid-containing sample, wherein the improvement comprises (a) mixing with the sample a finely-divided adsorbent selected from the group consisting of charcoal, silica gel, talc, bentonote, clay base materials, aluminia base materials, magnesia base materials, and cellulose base materials having a surface area of at least 50 square meters per gram and having an affinity for lipids in an amount sufficient to adsorb lipids present in said sample, and a nonionic, water-soluble polhydroxypolymer having a molecular weight of about 1,000 to 100,000 in an amount sufficient to accelerate precipitation of adsorbed lipids in said sample, (b) precipitating said adsorbent containing adsorbed lipids in said sample and (c) measuring said sample for said substance.
Claim 2
Dependent Claim
122 words
In a double antibody immunoassay for a substance in a lipid-containing sample, wherein the improvement comprises (a) mixing with the sample a finely-divided adsorbent selected from the group consisting of charcoal, silica gel, talc, bentonote, clay base materials, aluminia base materials, magnesia base materials, and cellulose base materials having a surface area of at least 50 square meters per gram and having an affinity for lipids in an amount sufficient to adsorb lipids present in said sample, and a nonionic, water-soluble polhydroxypolymer having a molecular weight of about 1,000 to 100,000 in an amount sufficient to accelerate precipitation of adsorbed lipids in said sample, (b) precipitating said adsorbent containing adsorbed lipids in said sample and (c) measuring said sample for said substance.
Claim 3
Dependent Claim
16 words
The assay of claim 1 wherein said sample is selected from blood serum or blood plasma.
Claim 4
Dependent Claim
19 words
The assay of claim 1 wherein said adsorbent has an average particle size of less than about 50 microns.
Claim 5
Dependent Claim
39 words
The assay of claim 1 wherein said adsorbent and said polymer are added to said sample simultaneously in a lipid-adsorbing reagent containing said adsorbent and said polymer and an aqueous solution having a pH of about 4 to 10.
Claim 6
Dependent Claim
24 words
The assay of claim 4 wherein said lipid-adsorbing reagent is prepared by ultrasonic mixing of said adsorbent and said polymer in said aqueous solution.
Claim 7
Dependent Claim
54 words
The assay of claim 1 wherein said adsorbent and said polymer are added to said sample as separate reagents, an adsorbent-containing reagent and a polymer-containing reagent, wherein the adsorbent and polymer is each present in its said reagent and said reagents further include an aqueous solution having a pH of about 4 to 10.
Claim 8
Dependent Claim
41 words
The assay of claim 1 wherein the amount of said adsorbent is less than about 40 milligrams of adsorbent per milliliter of sample and the amount of said polymer is less than about 100 milligrams of polymer per milliliter of sample.
Claim 9
Dependent Claim
16 words
The assay of claim 1 wherein said assay is radioimmunoassay and said substance is prostatic phosphatase.
Claim 10
Dependent Claim
17 words
The assay of claim 1 wherein said assay is a radioimmunoassay and said substance is creatine phosphokinase.
Claim 11
Dependent Claim
15 words
The assay of claim 1 wherein said measuring is a photometric measurement of enzyme activity.
Claim 12
Dependent Claim
11 words
The assay of claim 1 wherein said polymer is polyethylene glycol.
Claim 13
Dependent Claim
14 words
The assay of claim 1 wherein the substance to be assayed is an antigen.
Claim 14
Dependent Claim
71 words
A reagent useful for counteracting lipemic interference in double antibody immunoassay of a lipid-containing sample, which comprises: a finely-divided adsorbent having a surface area of at least 50 square meters per gram and having a high affinity for lipids in an amount sufficient to adsorb lipids; and polyethylene glycol having a molecular weight of about 1,000 to 100,000 in an amount sufficient to accelerate precipitation of adsorbed lipids in said sample.
Claim 15
Dependent Claim
71 words
A reagent useful for counteracting lipemic interference in double antibody immunoassay of a lipid-containing sample, which comprises: a finely-divided adsorbent having a surface area of at least 50 square meters per gram and having a high affinity for lipids in an amount sufficient to adsorb lipids; and polyethylene glycol having a molecular weight of about 1,000 to 100,000 in an amount sufficient to accelerate precipitation of adsorbed lipids in said sample.
Claim 16
Dependent Claim
29 words
The reagent of claim 13 wherein said adsorbent is one having an average particle size of less than about 20 microns and is selected from silica gel and talc.
Claim 17
Dependent Claim
26 words
The reagent of claim 13 wherein said adsorbent and said polyethylene glycol are in an aqueous solution having a buffered ph of about 4 to 10.
References Cited (Backward Citations)
US3899298A
Cited
US4056468A
Cited
US3954409A
Cited
US4148869A
Cited
US4028465A
Cited
US4121975A
Cited
US4166104A
Cited
US4111657A
Cited
US4139604A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |