Abstract Apparatus for determination of Angiotensin I comprises a vial of 125I Angiotensin I containing anion exchange resin, a vial of Angiotensin I standard, at least one vial of Angiotensin I Antiserum, and the following reagents: tris(hydroxymethyl)aminomethane, powdered charcoal, barbital buffer mixture, 8-hydroxyquinoline sulfate, bovine serum albumin powder, and Dimercaprol solution. The method involves the radioimmunoassay of Angiotensin I generated by the action of renin in incubated plasma samples. Antibody to Angiotensin I is employed as the specific antibody, and 125I Angiotensin is employed as the labeled antigen, and synthetic Angiotensin I is employed as a reference standard. Powdered charcoal is used to separate free Angiotensin I from antibody-bound Angiotensin I.
APPARATUS FOR DETERMINATION OF ANGIOTENSIN 1 COMPRISING A CARRIER BEING COMPARTMENTED TO RECEIVE AT LEAST THREE VIALS AND TO MAINTAIN SAID VIALS IN CLOSE CONFINEMENT, A FIRST VIAL COMPRISING AN ACETIC ACID SOLUTION CONTAINING ABOUT 10 PICOGRAMS OF ANGIOTENSIN I PER MICROLITER, A SECOND VIAL OF ANGIOTENSIN I ANTISERUM, AND A THIRD VITAL COMPRISING AN AQUEOUS SOLUTION OF 125I ANGIOTENSIN I HAVING A TOTAL ACTIVITY OF ABOUT 2 MICROCURIES, AND HAVING THEREIN A STRIP OF PAPER, SAID PAPER IMPREGNATED WITH AN ANION EXCHANGE RESIN.
Claim 2
Dependent Claim
84 words
APPARATUS FOR DETERMINATION OF ANGIOTENSIN 1 COMPRISING A CARRIER BEING COMPARTMENTED TO RECEIVE AT LEAST THREE VIALS AND TO MAINTAIN SAID VIALS IN CLOSE CONFINEMENT, A FIRST VIAL COMPRISING AN ACETIC ACID SOLUTION CONTAINING ABOUT 10 PICOGRAMS OF ANGIOTENSIN I PER MICROLITER, A SECOND VIAL OF ANGIOTENSIN I ANTISERUM, AND A THIRD VITAL COMPRISING AN AQUEOUS SOLUTION OF 125I ANGIOTENSIN I HAVING A TOTAL ACTIVITY OF ABOUT 2 MICROCURIES, AND HAVING THEREIN A STRIP OF PAPER, SAID PAPER IMPREGNATED WITH AN ANION EXCHANGE RESIN.
Claim 3
Dependent Claim
16 words
Apparatus in accordance with claim 1 which contains a plurality of vials of Angiotensin I antiserum.
Claim 4
Dependent Claim
14 words
Apparatus in accordance with claim 2 which contains 5 vials of Angiotensin I antiserum.