US4121975A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Pretreatment of samples for polyiodothyronine assays
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1976-08-20
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Granted: 1978-10-24
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Expires: 1996-08-20
Abstract
Abstract Composition and method are provided for the pretreatment of serum prior to a thyroxine assay. Prior to carrying out of the assay, particularly an immunoassay or competitive protein-binding assay, the serum sample is combined with cyclodextrin preferably in combination with an hydroxylated aromatic carboxylic acid in a basic medium, in amounts sufficient to minimize interference with obtaining an accurate and reliable determination of polyiodothyronine in the serum. Normally, after the reagents have been added to the serum, a small incubation period is allowed, after which time the additional reagents may be added for carrying out the assay.
Inventors
- 👤 Edwin F. Ullman
- 👤 Joel E. Lavine
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 17:12
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
28 words
A composition useful for the treatment of serum prior to thyroxine determination comprising an alkaline aqueous solution having α-cyclodextrin and salicylate in a weight ratio of about 5-20:1.
Claim 2
Dependent Claim
28 words
A composition useful for the treatment of serum prior to thyroxine determination comprising an alkaline aqueous solution having α-cyclodextrin and salicylate in a weight ratio of about 5-20:1.
Claim 3
Dependent Claim
20 words
A composition according to claim 1 wherein the alkaline solution has an hydroxyl concentration in the range of about 0.25-2N.
Claim 4
Dependent Claim
28 words
A composition according to claim 2, wherein said weight ratio of α-cyclodextrin to salicylate is about 10:1 and said hydroxyl concentration is in the range from about 0.25-0.75N.
Claim 5
Dependent Claim
42 words
In a method for carrying out a competitive protein binding assay or a homogeneous enzyme immunoassay for polyiodothyronine in serum, the improvement which comprises adding to said serum prior to said determination, cyclodextrin to provide a final concentration 0.1-5 weight percent cyclodextrin.
Claim 6
Dependent Claim
42 words
In a method for carrying out a competitive protein binding assay or a homogeneous enzyme immunoassay for polyiodothyronine in serum, the improvement which comprises adding to said serum prior to said determination, cyclodextrin to provide a final concentration 0.1-5 weight percent cyclodextrin.
Claim 7
Dependent Claim
41 words
A method according to claim 4, wherein said cyclodextrin is alpha, and a base and salicylate are added to said serum to provide a final concentration in said serum of about 0.1-1.5N hydroxide and about 0.5 to 5 mg/ml of salicylate.
Claim 8
Dependent Claim
33 words
A method according to claim 5, wherein the hydroxide is present in from 0.2-0.4N, the α-cyclodextrin is present in from about 1.5-4 weight percent and the salicylate is present in from about 1-4mg/ml.
Claim 9
Dependent Claim
21 words
A method according to claim 5 including the step of incubating the sample after addition of said base, α-cyclodextrin and salicylate.
Claim 10
Dependent Claim
14 words
A method according to claim 5, wherein said method is a homogeneous enzyme immunoassay.
Claim 11
Dependent Claim
15 words
A method according to claim 5, wherein said method is a competitive protein binding assay.
Claim 12
Dependent Claim
14 words
A method according to claim 4, wherein said method is a homogeneous enzyme immunoassay.
Claim 13
Dependent Claim
15 words
A method according to claim 4, wherein said method is a competitive protein binding assay.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-23 | 1982-04-24 | 1982-10-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-23 | 1986-04-24 | 1986-10-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-23 | 1990-04-24 | 1990-10-24 | $7700.00 | Window Closed / Lapsed |