Patent US4264587A successfully refreshed from Google Patents.
US4264587A
Expired / Lapsed
US
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Vaccine for preventing persistent feline leukemia viremia in cats
Assignee: Individual
•
Filing: 1979-08-01
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Granted: 1981-04-28
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Expires: 1999-08-01
Abstract
Abstract A vaccine in parenteral dose form for preventing persistent feline leukemia viremia in cats is characterized by containing as the immunizing agent inactivated feline leukemia virus (FeLV) derived from the FL74 feline lymphoblastoid cell line.
Inventors
- 👤 Niels C. Pedersen
- 👤 Gordon H. Theilen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 07:48
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/13011; C12N2740/13022; Y; Y10; Y10S; Y10S424/00; Y10S424/819
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
107 words
The method of protecting cats and kittens against diseases resulting from infection with feline leukemia virus (FeLV) in which FeLV is obtained by culturing FL74 feline lymphoblastoid cells, separating the FeLV from the cells, inactivating the FeLV, and vaccinating the cat or kitten with inactivated FeLV, wherein the improvement comprises inactivating the FeLV by contact with an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde, and protecting the cat or kitten against persistent leukemia viremia by vaccinating the cat or kitten with a plurality of successive doses of from 0.01 to 0.8 milligrams (mg) per dose of said inactivated FeLV (protein basis).
Claim 2
Independent Claim
107 words
The method of protecting cats and kittens against diseases resulting from infection with feline leukemia virus (FeLV) in which FeLV is obtained by culturing FL74 feline lymphoblastoid cells, separating the FeLV from the cells, inactivating the FeLV, and vaccinating the cat or kitten with inactivated FeLV, wherein the improvement comprises inactivating the FeLV by contact with an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde, and protecting the cat or kitten against persistent leukemia viremia by vaccinating the cat or kitten with a plurality of successive doses of from 0.01 to 0.8 milligrams (mg) per dose of said inactivated FeLV (protein basis).
Claim 3
Dependent Claim
29 words
The improved method of claim 1 in which each vaccine dose contains from 0.02 to 0.4 mg of said FeLV (protein basis) and said doses are administered to kittens.
Claim 4
Dependent Claim
24 words
The improved method of claim 1 or claim 2 in which said FeLV is obtained by culturing the FL74-UCD-1 cell line (ATCC No. CRL-8012).
Claim 5
Independent Claim
118 words
The method of protecting kittens against diseases resulting from infection with feline leukemia virus (FeLV) in which FeLV is obtained by culturing FL74 feline lymphoblastoid cells, separating the FeLV from the cells, inactivating the FeLV, and vaccinating the cat or kitten with the inactivated FeLV in admixture with an adjuvant, wherein the improvement comprises protecting the cat or kitten against persistent leukemia viruemia by the steps of inactivating the FeLV obtained from said culturing by introducing the FeLV into an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde, and vaccinating the cat or kitten with a plurality of successive doses of from 0.02 to 0.4 milligrams (mg) of said inactivated FeLV (protein basis).
Claim 6
Independent Claim
118 words
The method of protecting kittens against diseases resulting from infection with feline leukemia virus (FeLV) in which FeLV is obtained by culturing FL74 feline lymphoblastoid cells, separating the FeLV from the cells, inactivating the FeLV, and vaccinating the cat or kitten with the inactivated FeLV in admixture with an adjuvant, wherein the improvement comprises protecting the cat or kitten against persistent leukemia viruemia by the steps of inactivating the FeLV obtained from said culturing by introducing the FeLV into an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde, and vaccinating the cat or kitten with a plurality of successive doses of from 0.02 to 0.4 milligrams (mg) of said inactivated FeLV (protein basis).
Claim 7
Dependent Claim
26 words
The improved method of claim 4 in which the formaldehyde concentration of said aqueous solution used from said FeLV inactivation is from 0.5 to 1.5% formaldehyde.
Claim 8
Dependent Claim
24 words
The improved method of claim 4 or claim 5 in which said FeLV is obtained by culturing the FL74-UCD-1 cell line (ATCC No. CRL-8012).
Claim 9
Dependent Claim
19 words
The improved method of claim 4, claim 5, or claim 6 in which said adjuvant is Freund's incomplete adjuvant.
Claim 10
Dependent Claim
33 words
The improved method of claim 4 or claim 5 in which said FeLV is obtained by culturing the FL74-UCD-1 cell line (ATCC No. CRL-8012), and in which said adjuvant is Freund's incomplete adjuvant.
Claim 11
Dependent Claim
76 words
The process of preparing a vaccine for prevention of persistent feline leukemia viremia, in which FL74 feline lymphoblastoid cells are cultured in a medium with elaboration of feline leukemia virus (FeLV) into the medium, separating the cells from the medium, and recovering the active FeLV, wherein the improvement comprises enhancing the antigenic response to said vaccine by inactivating the recovered FeLV in an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde.
Claim 12
Dependent Claim
76 words
The process of preparing a vaccine for prevention of persistent feline leukemia viremia, in which FL74 feline lymphoblastoid cells are cultured in a medium with elaboration of feline leukemia virus (FeLV) into the medium, separating the cells from the medium, and recovering the active FeLV, wherein the improvement comprises enhancing the antigenic response to said vaccine by inactivating the recovered FeLV in an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde.
Claim 13
Dependent Claim
20 words
The process improvement of claim 9 in which said aqueous solution has a formaldehyde concentration of from 0.5 to 1.5%.
Claim 14
Dependent Claim
24 words
The process improvement of claim 9 or claim 10 in which said FL74 cells are provided by the FL74-UCD-1 cell line (ATCC No. CRL-8012).
Claim 15
Dependent Claim
117 words
The process of preparing a vaccine for prevention of persistent feline leukemia viremia, in which FL74 feline lymphoblastoid cells are cultured in a medium with elaboration of feline leukemia virus (FeLV) into the medium, separating the cells from the medium, and recovering the active FeLV, wherein the improvement comprises enhancing the antigenic potency of said vaccine by the steps of inactivating the recovered FeLV in an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde, and preparing the inactivated FeLV in injectable dose form by combining the FeLV with an adjuvant therefor, said injectable dose form containing from 0.1 to 0.8 milligrams (mg) of FeLV (protein basis) per 0.5 milliliter (ml) of dose.
Claim 16
Dependent Claim
117 words
The process of preparing a vaccine for prevention of persistent feline leukemia viremia, in which FL74 feline lymphoblastoid cells are cultured in a medium with elaboration of feline leukemia virus (FeLV) into the medium, separating the cells from the medium, and recovering the active FeLV, wherein the improvement comprises enhancing the antigenic potency of said vaccine by the steps of inactivating the recovered FeLV in an aqueous solution of formaldehyde having a concentration of from 0.3 to 1.8% formaldehyde, and preparing the inactivated FeLV in injectable dose form by combining the FeLV with an adjuvant therefor, said injectable dose form containing from 0.1 to 0.8 milligrams (mg) of FeLV (protein basis) per 0.5 milliliter (ml) of dose.
Claim 17
Dependent Claim
11 words
The vaccine dose form prepared by the process of claim 12.
Claim 18
Dependent Claim
29 words
The improved process of claim 24 or the vaccine dose of claim 13 in which said dose form is prepared by combining said adjuvant with said FeLV-containing formaldehyde solution.
Claim 19
Dependent Claim
130 words
The process of preparing a vaccine for preparation of persistent feline leukemia viremia, in which FL74 feline lymphoblastoid cells are cultured in a medium with elaboration of feline leukemia virus (FeLV) into the medium separating the cells from the medium, and recovering the active FeLV, wherein the improvement comprises enhancing the antigenic potency of said vaccine by the steps of using the FL74-UCD-1 cell line (ATCC No. CRL-8012) to prepare said active FeLV, inactivating the recovered FeLV in an aqueous solution of formaldehyde having a concentration of from 0.5 to 1.5% formaldehyde, and preparing the inactivated FeLV in injectable dose form by combining the FeLV with Freund's incomplete adjuvant, said injectable dose form containing from 0.2 to 0.4 milligrams (mg) of FeLV (protein basis) per 0.5 milliliter (ml) of dose.
Claim 20
Dependent Claim
130 words
The process of preparing a vaccine for preparation of persistent feline leukemia viremia, in which FL74 feline lymphoblastoid cells are cultured in a medium with elaboration of feline leukemia virus (FeLV) into the medium separating the cells from the medium, and recovering the active FeLV, wherein the improvement comprises enhancing the antigenic potency of said vaccine by the steps of using the FL74-UCD-1 cell line (ATCC No. CRL-8012) to prepare said active FeLV, inactivating the recovered FeLV in an aqueous solution of formaldehyde having a concentration of from 0.5 to 1.5% formaldehyde, and preparing the inactivated FeLV in injectable dose form by combining the FeLV with Freund's incomplete adjuvant, said injectable dose form containing from 0.2 to 0.4 milligrams (mg) of FeLV (protein basis) per 0.5 milliliter (ml) of dose.
Claim 21
Dependent Claim
24 words
The improved process of claim 15 in which said dose form is prepared by combining said Freund's incomplete adjuvant with said FeLV-containing formaldehyde solution.
Claim 22
Dependent Claim
14 words
The vaccine dose form prepared by the process of claim 15 or claim 16.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-04-27 | 1984-10-27 | 1985-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-04-27 | 1988-10-27 | 1989-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-04-27 | 1992-10-27 | 1993-04-28 | $7700.00 | Window Closed / Lapsed |