US4205128A
Expired / Lapsed
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Process for producing immobilized enzyme compositions
Assignee: Denka Co Ltd
•
Filing: 1978-08-25
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Granted: 1980-05-27
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Expires: 1998-08-25
Abstract
Abstract Immobilized enzyme compositions for industrial use are produced by reacting an enzyme and/or enzyme-containing microorganism cells with an anion-exchange high molecular substance having a quaternary pyridine ring in the molecule, and then subjecting the reaction products to molding and drying in a molding machine. A polyfunctional crosslinking agent may be added at any stage of the process. The immobilized enzyme compositions thus prepared can be packed in a reactor of an industrial scale for continuous enzyme reaction.
Inventors
- 👤 Yoshiaki Ishimatsu
- 👤 Shigeki Shigesada
- 👤 Hiroyasu Suzuki
- 👤 Hironoshin Kitagawa
- 👤 Shoji Kimura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:52
CPC Classifications
C; C08; C08F; C08F8/00; B; B29; B29C; B29C48/00; B29C48/022; B29C48/03; B29C48/04; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
155 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with a cross-linking agent and an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.05, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C, adjusting the water contents of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding the adjusted reaction products with an extruder at a temperature from about 0° C. to 25° C., thereby obtaining an extrudate, pelletizing said extrudate, and then drying the pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours.
Claim 2
Dependent Claim
155 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with a cross-linking agent and an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.05, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C, adjusting the water contents of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding the adjusted reaction products with an extruder at a temperature from about 0° C. to 25° C., thereby obtaining an extrudate, pelletizing said extrudate, and then drying the pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours.
Claim 3
Dependent Claim
24 words
The process of claim 1 wherein the enzyme-containing microorganic cells are selected from the group consisting of cells containing glucose isomerase, glucoamylase and aminoacylase.
Claim 4
Dependent Claim
20 words
The process of claim 1 wherein said enzyme is selected from the group consisting of glucose isomerase, glucoamylase and aminoacylase.
Claim 5
Dependent Claim
15 words
The process of claim 1, wherein the extruder is a twin screw horizontal type extruder.
Claim 6
Dependent Claim
160 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C., adjusting the water content of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding the adjusted reaction products in the presence of a cross-linking agent with an extruder at a temperature of from about 0° C. to 25° C., thereby obtaining an extrudate, pelletizing said extrudate, and then drying the resulted pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours.
Claim 7
Dependent Claim
160 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C., adjusting the water content of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding the adjusted reaction products in the presence of a cross-linking agent with an extruder at a temperature of from about 0° C. to 25° C., thereby obtaining an extrudate, pelletizing said extrudate, and then drying the resulted pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours.
Claim 8
Dependent Claim
161 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C., adjusting the water content of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding said adjusted products with an extruder at a temperature from about 0° C. to 25° C., thereby obtaining an extrudate, contacting said extrudate with a solution of a cross-linking agent, pelletizing the resulting extrudate, and then drying the pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours.
Claim 9
Dependent Claim
161 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary pyridine ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C., adjusting the water content of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding said adjusted products with an extruder at a temperature from about 0° C. to 25° C., thereby obtaining an extrudate, contacting said extrudate with a solution of a cross-linking agent, pelletizing the resulting extrudate, and then drying the pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours.
Claim 10
Dependent Claim
162 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C., adjusting the water contents of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding the adjusted reaction products with an extruder at a temperature from about 0° C. to 25° C., thereby obtaining an extrudate, pellitizing said extrudate, and then drying the pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours, and contacting said dried pellets with a solution of a cross-linking agent.
Claim 11
Dependent Claim
162 words
A process for producing an immobilized enzyme composition which comprises the steps of reacting enzyme and/or enzyme containing microorganic cells with an anionic exchange high molecular substance containing a quaternary ring in the molecule at a weight ratio of the enzyme and/or enzyme containing microorganic cells to the anionic exchange high molecular substance of between 1:0.005 and 1:0.5, in an aqueous dispersion having a pH range of from about 5 to 9, at a temperature from about 0° C. to about 30° C., adjusting the water contents of the resulting reaction products to from about 25 to about 80 wt.%, subjecting to extrusion molding the adjusted reaction products with an extruder at a temperature from about 0° C. to 25° C., thereby obtaining an extrudate, pellitizing said extrudate, and then drying the pellets at a temperature from about 40° C. to 80° C. for about 15 minutes to 20 hours, and contacting said dried pellets with a solution of a cross-linking agent.
Claim 12
Dependent Claim
22 words
The process of any of claims 1, 5, 6 or 7 wherein said extruder has a screen of 0.1 to 2.0 mm.
Claim 13
Dependent Claim
17 words
The process any of claims 1, 5, 6 or 7, wherein the crosslinking agent is dialdehyde starch.
Claim 14
Dependent Claim
16 words
The process any of claims 1, 5, 6 or 7, wherein the crosslinking agent is glutaraldehyde.
Claim 15
Dependent Claim
43 words
The process any of claims 1, 5, 6 or 7, wherein the crosslinking agent is selected from the group consisting of condensation products of ureaformaldehyde, succinic anhydride, maleic anhydride copolymer, epichlorohydrin, epoxy resin, cyanuric acid chloride, aminodichlorotriazine, toluene diisocyanate, dichlorobutene and Woodward's reagent.
Claim 16
Dependent Claim
38 words
The process any of claims 1, 5, 6 or 7, wherein the weight ratio of the enzyme and/or enzyme containing microorganic cells to the anion exchange high molecular substance is in the range of from 1:0.05 to 1:0.2.
Claim 17
Dependent Claim
13 words
The product of the process any of claims 1, 5, 6 or 7.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-05-27 | 1983-11-26 | 1984-05-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-05-27 | 1987-11-26 | 1988-05-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-05-27 | 1991-11-26 | 1992-05-27 | $7700.00 | Window Closed / Lapsed |