US4001264A
Expired / Lapsed
US
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Enzyme complexes and their use
Assignee: Beecham Group PLC
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Filing: 1974-12-12
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Granted: 1977-01-04
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Expires: 1994-12-12
Abstract
Abstract Novel water-insoluble enzyme complexes are provided which comprise a penicillin acylase enzyme adsorbed on a water-insoluble polymer substrate which is a polymer or copolymer of methacrylic acid and cross-linked with a cross-linking agent selected from glutaraldehyde, glyoxal and formaldehyde. The complexes are useful in the production of 6-APA from penicillins G and V.
Inventors
- 👤 Thomas Adrian Savidge
- 👤 Lawson William Powell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 10:52
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
89 words
In the process for preparing 6-aminopenicillanic acid, which process consists essentially of maintaining an aqueous solution of benzylpenicllin or phenoxymethylpenicillin or a salt thereof at a pH of from 6.0 to 9.0 while treating it with a water-insoluble enzyme complex, wherein a penicillin acylase enzyme has been adsorbed on a water-insoluble polymer substrate and cross-linked with a cross-linking agent selected from the group consisting of glutaraldehyde, glyoxal and formaldehyde, the improvement wherein the said polymer substrate for the said enzyme is a water-insoluble polymer or coploymer of methacrylic acid.
Claim 2
Dependent Claim
89 words
In the process for preparing 6-aminopenicillanic acid, which process consists essentially of maintaining an aqueous solution of benzylpenicllin or phenoxymethylpenicillin or a salt thereof at a pH of from 6.0 to 9.0 while treating it with a water-insoluble enzyme complex, wherein a penicillin acylase enzyme has been adsorbed on a water-insoluble polymer substrate and cross-linked with a cross-linking agent selected from the group consisting of glutaraldehyde, glyoxal and formaldehyde, the improvement wherein the said polymer substrate for the said enzyme is a water-insoluble polymer or coploymer of methacrylic acid.
Claim 3
Dependent Claim
21 words
The process according to claim 1, wherein the penicillin acylase enzyme purity is in the range 1.5 to 30 micromoles/min/mg protein.
Claim 4
Dependent Claim
19 words
The process according to claim 1, wherein the substrate is a macroporous copolymer of methacrylic acid and divinyl benzene.
Claim 5
Dependent Claim
28 words
The process according to claim 1, wherein the substrate is a gel copolymer of methacrylic acid and divinyl benzene which also contains a proportion of benzene sulphonyl groups.
Claim 6
Dependent Claim
12 words
The process according to claim 1, wherein the crosslinking agent is glutaraldehyde.
Claim 7
Dependent Claim
29 words
The process according to claim 1, wherein the enzyme complex is in the form of finely-divided particles or beads of particle size in the range 0.1 to 0.01 mm.
Claim 8
Dependent Claim
61 words
The process according to claim 1, wherein the penicillin acylase enzyme purity is in the range 1.5 to 30 micromoles/min/mg protein, the substrate is a macroporous copolymer of methacrylic acid and divinyl benzene, the cross-linking agent is glutaraldehyde and the enzyme complex is in the form of finely-divided particles or beads of particle size in the range 0.1 to 0.01 mm.
Claim 9
Dependent Claim
38 words
The process according to claim 7, wherein the reaction temperature is in the range 30° - 50° C, and the pH is maintained in the range 7.0 to 8.5 by the continuous or intermittent addition of sodium hydroxide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-01-04 | 1980-07-05 | 1981-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-01-04 | 1984-07-05 | 1985-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-01-04 | 1988-07-05 | 1989-01-04 | $7700.00 | Window Closed / Lapsed |