US3915797A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilized enzymes
Assignee: Denka Co Ltd
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Filing: 1974-02-20
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Granted: 1975-10-28
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Expires: 1994-02-20
Abstract
Abstract A water-insoluble enzymatically active composition is produced by causing a biologically active enzyme or enzymatically active microorganic cells to be adsorbed on an anion-exchange-resin having a quaternary pyridine ring in the molecular unit thereof. A method for the preparation of this composition and a method for the use thereof are also disclosed.
Inventors
- 👤 Yoshiaki Ishimatsu
- 👤 Shigeki Shigesada
- 👤 Shoji Kimura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 21:12
CPC Classifications
B; B01; B01J; B01J31/00; B01J31/003; B01J31/02; B01J31/06; B01J31/08; C; C08; C08F; C08F8/00; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; C12P; C12P13/00; C12P13/04; C12P19/00; C12P19/24
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
149 words
A WATER-INSOLUBLE, ENZYMATICALLY ACTIVE IMMOBILIZED ENZYME COMPOSITION COMPRISING A BIOLOGICALLY ACTIVE ENZYME SUBSTANCE BONDED TO A SUBSTANTIALLY WATER-INSOLUBLE ANION EXCHANGE RESIN HAVING A QUATERNIZED NITROGEN ATOM CONTAINED IN A PYRIDINE RING IN THE MOLECULAR UNIT THEREOF, SAID ANION EXCHANGE RESIN BEING JORMED BY ALLOWING A REAGENT CAPABLE OF QUATERNIZING THE NITROGEN ATOM IN SAID PYRIDINE RING AND SELECTED FORM THE GROUP CONSISTING OF METHYL CHLORIDE, METHY BROMIDE, METHYL IODIDE AND DIMETHYL SULFATE TO REACT UPON A COPOLYMER WHICH IS OBTAINED BY COPOLYMERIZING A VINYL PYRIDINE, DINE SELECTED FROM THE GROUP CONSISTING OF 4-VINYL PYRIDINE, 2-VINYL PYRIDINE, 5-METHYL-2-VINYL PYRIDINE, 3-ALLYL PYRIDINE, 1-VINYL QUINOLINE AND 2-METHYL-5-VINYL PYRIDINE AND AT LEAST ONE MONOMER SELECTED FROM THE GROUP CONSISTING OF AROMATIC VINYL COMPOUNDS, ETHYLENICALLY UNSATURATED COMPOUNDS, DIENE-UNSATURATED COMPOUNDS AND DIVINYL-UNSATURATED COMPOUNDS WHICH ARE COPOLYMERIZABLE WITH SAID VINYL PYRIDINE SAID VINYL PYRIDINE BEING PRESENT IN AN AMOUNT OF FROM 20 TO 99 MOL %.
Claim 2
Dependent Claim
149 words
A WATER-INSOLUBLE, ENZYMATICALLY ACTIVE IMMOBILIZED ENZYME COMPOSITION COMPRISING A BIOLOGICALLY ACTIVE ENZYME SUBSTANCE BONDED TO A SUBSTANTIALLY WATER-INSOLUBLE ANION EXCHANGE RESIN HAVING A QUATERNIZED NITROGEN ATOM CONTAINED IN A PYRIDINE RING IN THE MOLECULAR UNIT THEREOF, SAID ANION EXCHANGE RESIN BEING JORMED BY ALLOWING A REAGENT CAPABLE OF QUATERNIZING THE NITROGEN ATOM IN SAID PYRIDINE RING AND SELECTED FORM THE GROUP CONSISTING OF METHYL CHLORIDE, METHY BROMIDE, METHYL IODIDE AND DIMETHYL SULFATE TO REACT UPON A COPOLYMER WHICH IS OBTAINED BY COPOLYMERIZING A VINYL PYRIDINE, DINE SELECTED FROM THE GROUP CONSISTING OF 4-VINYL PYRIDINE, 2-VINYL PYRIDINE, 5-METHYL-2-VINYL PYRIDINE, 3-ALLYL PYRIDINE, 1-VINYL QUINOLINE AND 2-METHYL-5-VINYL PYRIDINE AND AT LEAST ONE MONOMER SELECTED FROM THE GROUP CONSISTING OF AROMATIC VINYL COMPOUNDS, ETHYLENICALLY UNSATURATED COMPOUNDS, DIENE-UNSATURATED COMPOUNDS AND DIVINYL-UNSATURATED COMPOUNDS WHICH ARE COPOLYMERIZABLE WITH SAID VINYL PYRIDINE SAID VINYL PYRIDINE BEING PRESENT IN AN AMOUNT OF FROM 20 TO 99 MOL %.
Claim 3
Dependent Claim
24 words
An immobilized enzyme composition according to claim 1, wherein the anion-exchange-resin has a total anion-exchange capacity of the order of 2.0 to 5.0 meq/g.
Claim 4
Dependent Claim
18 words
An immobilized enzyme composition according to claim 1, wherein the divinyl-unsaturated compounds include divinylbenzene and (poly)ethylene glycol dimethacrylate.
Claim 5
Dependent Claim
19 words
An immobilized enzyme composition according to claim 1, wherein the preparation of the copolymer is effected by radical polymerization.
Claim 6
Dependent Claim
21 words
An immobilized enzyme composition according to claim 1, wherein the preparation of the copolymer is effected by block or graft polymerization.
Claim 7
Dependent Claim
21 words
An immobilized enzyme composition according to claim 5, wherein vinylpyridine is present in an amount of from 25 to 75 mol%.
Claim 8
Dependent Claim
36 words
An immobilized enzyme composition according to claim 1, wherein the biologically active enzyme substance is selected from the group consisting of aminoacylase, glucoamylase, glucose isomerase, glucose oxidase, alkaline protease, invertase and microorganic cells containing said enzymes.
Claim 9
Dependent Claim
25 words
An immobilized enzyme composition according to claim 1 wherein said monomer is a divinyl-unsaturated compound in an amount of from 0.5 to 30 mol %.
Claim 10
Dependent Claim
21 words
A method for continuously converting a substrate by use of the water-insoluble, enzymatically active immobilized enzyme composition according to claim 1.
Claim 11
Dependent Claim
23 words
A method according to claim 9, wherein an immobilized aminoacylase composition is used to effect continuous conversion of N-acetyl-DL-amino acid into L-amino acid.
Claim 12
Dependent Claim
21 words
A method according to claim 9, wherein an immobilized glucoamylase composition is used to effect continuous conversion of starch into glucose.
Claim 13
Dependent Claim
22 words
A method according to claim 9, wherein an immobilized glucose isomerase composition is used to effect continuous conversion of glucose into fructose.
Claim 14
Dependent Claim
23 words
A method according to claim 9, wherein an immobilized glucose oxidase composition is used to effect continuous conversion of glucose into gluconic acid.
Claim 15
Dependent Claim
23 words
A method according to claim 9, wherein an immobilized invertase composition is used to effect continuous conversion of sucrose into glucose and fructose.
References Cited (Backward Citations)
US3563921A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-10-27 | 1979-04-28 | 1979-10-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-10-27 | 1983-04-28 | 1983-10-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-10-27 | 1987-04-28 | 1987-10-28 | $7700.00 | Window Closed / Lapsed |