Patent US4298806A successfully refreshed from Google Patents.
US4178285A
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Separation of active α1 -acid glycoprotein and utilization in the lipoprotein lipase enzyme system
Assignee: Individual
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Filing: 1978-12-20
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Granted: 1979-12-11
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Expires: 1998-12-20
Abstract
Abstract This invention relates to a method of separating the active α 1 -acid glycoprotein fraction, a co-factor in the lipoprotein lipase reaction, from urine of nephrotic animals and humans as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; and (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 O and lyophilize. This active α 1 -acid glycoprotein fraction is utilized in effective amounts in nephrotic animals to reverse the defect in triglyceride removal caused by the loss of plasma constituents in urine.
Inventors
- 👤 James M. Felts
- 👤 Ilona Staprans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 03:11
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/473; A; A61; A61K; A61K35/00; A61K35/12; A61K35/22; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/834
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
72 words
A method of separating the active α 1 -acid glycoprotein fraction, which contains a co-factor in the lipoprotein lipase reaction, from nephrotic urine as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 O and lyophilize.
Claim 2
Independent Claim
72 words
A method of separating the active α 1 -acid glycoprotein fraction, which contains a co-factor in the lipoprotein lipase reaction, from nephrotic urine as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; (c) recover the α 1 -acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO 3 , and purify by dialysis versus H 2 O and lyophilize.
Claim 3
Dependent Claim
26 words
The method of claim 1 wherein the α 1 -acid glycoprotein is recovered in the presence of an active glycosaminoglycan (GAG) with lipoprotein lipase co-factor activity.
Claim 4
Dependent Claim
32 words
The method of claim 1 wherein an active complex is produced embodying α 1 -acid glycoprotein in the presence of GAG, which complex may be further separated into active and inactive components.
Claim 5
Dependent Claim
16 words
The method of claim 1 wherein each procedure is performed in the cold at 0°-4° C.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-12-10 | 1983-06-11 | 1983-12-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-12-10 | 1987-06-11 | 1987-12-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-12-10 | 1991-06-11 | 1991-12-11 | $7700.00 | Window Closed / Lapsed |