US3901763A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the purification of glycero-lipid-splitting enzymes
Assignee: Toyo Jozo KK
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Filing: 1974-03-11
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Granted: 1975-08-26
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Expires: 1994-03-11
Abstract
Abstract Enzymes acting on glycero-lipids, for example, lipase, phospholipase, lysophospholipase or phosphatidate phosphatase, are purified by the steps of: contacting an aqueous enzyme solution with a carrier which is a fatty acid ester of a waterinsoluble polysaccharide or a derivative thereof containing hydroxyl groups, thereby to immobilize the same, and eluting the enzyme with a solution containing a surface active agent.
Inventors
- 👤 Yoshifumi Horiuchi
- 👤 Shigeyuki Imamura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 04:45
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/16; C12N9/18; C12N9/20; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/834; Y10S435/886; Y10S435/911; Y10S435/933; Y10S435/936
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
79 words
A METHOD FOR THE PURIFICATION OF AN ENZYME HAVING CUBSTRATE SPECIFICITY TO GLYCERO-LIPIDS SELECTED FROM THE GROUP CONSISTING OF GLYCERIDES AND GLYCERO-PHOSPHATIDES, WHICH COMPRISES THE STEPS OF: CONTACTING AN AQUEOUS SOLUTION OF A SAID ENZYME WITH A CARRIER SELECTED FROM THE GROUP CONSISTING OF FATTY ACID ESTERS OF WATER-INSOLUBLE POLYSACCHARIDES AND DERIVATIVES THEREOF CONTAINING HYDROXYL GROUPS, SAID FATTY ACID HAVING AT LEAST 6 CARBON ATOMS AND ELUTING THE ADSORBED ENZYME WITH AN AQUEOUS SOLUTION OF A SURFACE ACTIVE AGENT.
Claim 2
Independent Claim
79 words
A METHOD FOR THE PURIFICATION OF AN ENZYME HAVING CUBSTRATE SPECIFICITY TO GLYCERO-LIPIDS SELECTED FROM THE GROUP CONSISTING OF GLYCERIDES AND GLYCERO-PHOSPHATIDES, WHICH COMPRISES THE STEPS OF: CONTACTING AN AQUEOUS SOLUTION OF A SAID ENZYME WITH A CARRIER SELECTED FROM THE GROUP CONSISTING OF FATTY ACID ESTERS OF WATER-INSOLUBLE POLYSACCHARIDES AND DERIVATIVES THEREOF CONTAINING HYDROXYL GROUPS, SAID FATTY ACID HAVING AT LEAST 6 CARBON ATOMS AND ELUTING THE ADSORBED ENZYME WITH AN AQUEOUS SOLUTION OF A SURFACE ACTIVE AGENT.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-08-25 | 1979-02-24 | 1979-08-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-08-25 | 1983-02-24 | 1983-08-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-08-25 | 1987-02-24 | 1987-08-26 | $7700.00 | Window Closed / Lapsed |