US4119709A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diagnostic test
Assignee: Individual
•
Filing: 1976-02-17
•
Granted: 1978-10-10
•
Expires: 1996-02-17
Abstract
Abstract A diagnostic test is provided based on competitive binding in which a partition coefficient is established for the substance whose concentration is to be determined and for the radioactive labeled form of the substance between liquid and solid phases.
Inventors
- 👤 William R. Holub
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 05:11
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/53; G01N33/543; G01N33/54306; G01N33/82; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
176 words
A method of determining the concentration of a substance in a sample fluid by competitive binding, which comprises forming an admixture of a predetermined amount of said sample fluid, a predetermined amount of a radioactive isotope labeled form of said substance, a predetermined amount of a receptor capable of binding the labeled and unlabeled forms of the substance, and a predetermined amount of a particulate solid sorbent coated with a further amount of said receptor, said sorbent being precoated, before said admixture is formed, with said further amount of receptor, said further amount being at least sufficient to bind all of the labeled substance, allowing the resulting heterogenous liquid phase/solid phase system to equilibrate, said liquid phase comprising said substance bound to the receptor and said radioactive isotype bound to the receptor and said solid phase comprising said coated sorbent to which is bound said substance and said radioactive isotope, separating the liquid phase from the solid phase after equilibration, and counting with a radiation counter the radioactivity of the liquid phase or the solid phase.
Claim 2
Independent Claim
176 words
A method of determining the concentration of a substance in a sample fluid by competitive binding, which comprises forming an admixture of a predetermined amount of said sample fluid, a predetermined amount of a radioactive isotope labeled form of said substance, a predetermined amount of a receptor capable of binding the labeled and unlabeled forms of the substance, and a predetermined amount of a particulate solid sorbent coated with a further amount of said receptor, said sorbent being precoated, before said admixture is formed, with said further amount of receptor, said further amount being at least sufficient to bind all of the labeled substance, allowing the resulting heterogenous liquid phase/solid phase system to equilibrate, said liquid phase comprising said substance bound to the receptor and said radioactive isotype bound to the receptor and said solid phase comprising said coated sorbent to which is bound said substance and said radioactive isotope, separating the liquid phase from the solid phase after equilibration, and counting with a radiation counter the radioactivity of the liquid phase or the solid phase.
Claim 3
Dependent Claim
42 words
The method according to claim 1, wherein said solid sorbent is selected from the group consisting of kaolin, surcrose, starch, sodium carbonate, calcium carbonate, calcium phosphate, magnesium carbonate, calcium oxide, silicic acid, magnesium silicates, charcoal, magnesium oxide, aluminum oxide and fuller's earth.
Claim 4
Dependent Claim
12 words
The method according to claim 1, wherein said solid sorbent is kaolin.
Claim 5
Dependent Claim
33 words
The method according to claim 3, wherein the substance is L-thyroxine (T 4 ), the labeled form of the substance is T 4 -I 125 , and the receptor is thyroid binding globulin.
Claim 6
Dependent Claim
33 words
The method according to claim 3, wherein the substance is L-thyroxine (T 4 ), the labeled form of the substance is T 4 - 125 , and the receptor is T 4 -antibody.
Claim 7
Dependent Claim
33 words
The method according to claim 3, wherein the substance is triiodothyronine (T 3 ), the labeled form of the substance is T 3 -I 125 , and the receptor is T 3 -antibody.
Claim 8
Dependent Claim
26 words
The method according to claim 3, wherein the substance is Fe, the labeled form of the substance is Fe 59 , and the receptor is Transferrin.
Claim 9
Dependent Claim
29 words
The method according to claim 3, wherein the substance is thyroid stimulating hormone (TSH), the labeled form of the substance is TSH-I 125 , and the receptor is TSH-antibody.
Claim 10
Dependent Claim
28 words
The method according to claim 3, wherein the substance is digoxin, the labeled form of the substance is 3-o-succinyl digoxigen-I 125 tyrosine, and the receptor is digoxin antibody.
Claim 11
Dependent Claim
32 words
The method according to claim 3, wherein the substance is vitamin B 12 , the labeled form of the substance is CO 57 -cyanocobalamin, and the receptor is vitamin B 12 -antibody.
References Cited (Backward Citations)
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Cited
US3666854A
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US3768979A
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US3901654A
Cited
US3775615A
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US3776698A
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US3960492A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-09 | 1982-04-10 | 1982-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-09 | 1986-04-10 | 1986-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-09 | 1990-04-10 | 1990-10-10 | $7700.00 | Window Closed / Lapsed |