Patent US1576859A successfully refreshed from Google Patents.
US3775615A
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US
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Method of determining thyroid function
Assignee: NUCLEAR MED LAB
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Filing: 1971-07-06
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Granted: 1973-11-27
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Expires: 1991-07-06
Abstract
Abstract An improved method is provided for measuring the total amount of thyroid hormone within a body fluid. The procedure includes an extraction step whereby thyroid hormone (thyroxine) is initially extracted from a blood sample by first diluting the blood sample from 1.5 to 6 times its initial volume with a nonionic aqueous diluent and thereafter extracting the thyroxine with a solvent such as alcohol. The extracted thyroxine is then combined with a known amount of thyrobinding globulin and a radioisotope labeled thyroid hormone to form an equilibrated solution. The free thyroid hormone is extracted from the resulting equilibrated solution with a particulate inorganic crystalline lattice sorbent material. This invention relates to diagnostic tests for determining the level of thyroid hormone within a body fluid. In another aspect, this invention relates to an improved method for measuring total thyroid hormone within a body fluid. In a further aspect, this invention relates to an improved technique for extracting thyroid hormone from a blood serum sample.
Inventors
- 👤 A Eisentraut
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 15:25
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
13 words
The method of claim 1 wherein said nonionic aqueous diluent is distilled wAter.
Claim 2
Dependent Claim
6 words
labeled thyroxine remaining in said solution.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said organic solvent is an alcohol.
Claim 4
Dependent Claim
14 words
The method of claim 3 wherein said organic solvent comprises at least 90% ethanol.
Claim 5
Independent Claim
112 words
A method of measuring thyroxine in a serum sample comprising: a. diluting said serum sample from about 1.5 to about 6 times its original volume with a nonionic aqueous diluent; b. extracting thyroxine from said diluted serum sample of step (a) with an organic solvent; c. separating the extracted thyroxine from said organic solvent; d. equilibrating a buffer solution of said extracted thyroxine with known amounts of thyrobinding globulin and a radioactive isotope labeled thyroxine; e. thoroughly admixing the equilibrated solution of step (d) with a sorbent materail to remove free thyroxine therefrom; f. separating said sorbent material from the resulting solution; and g. counting with a scintillation well counter one of
Claim 6
Dependent Claim
13 words
The method of claim 5 wherein said nonionic aqueous diluent is distilled water.
Claim 7
Dependent Claim
36 words
The method of claim 5 wherein said extracting in step (c) comprises adding sufficent organic solvent to said diluted serum to yield a final organic solvent concentration therein of at least about 60 percent by volume.
Claim 8
Dependent Claim
51 words
The method of claim 7 wherein said sorbent material is a particulate, inorganic crystalline sorbent material which is selected from silicic acid and carbonates, phosphates, oxides, hydroxides, silicates, aluminates, and sulfates of the metallic elements of Groups IA, IIA, IIIA, IIB, and VIII of the Periodic Table, and mixed salts thereof.
Claim 9
Dependent Claim
13 words
The method of claim 8 wherein said nonionic aqueous diluent is distilled water.
Claim 10
Dependent Claim
12 words
The method of claim 9 wherein said organic solvent is an alcohol.
Claim 11
Dependent Claim
15 words
The method of claim 10 wherein said organic solvent comprises at least 90 percent ethanol.
Claim 12
Dependent Claim
143 words
In a method of determining the amount of thyroid hormone in a serum sample whereby the thyroid hormone is initially extracted from said sample with a solvent, and then removed from said solvent, and thereafter admixed with known amounts of thyrobinding globulin and a radioactive isotope labeled thyroid hormone, and then the resulting mixture is contacted with a sorbent material to remove free thyroid hormone therefrom and either the solvent or the remaining supernatant is counted in a scintillation counter, the improvement comprising: stabilizing the amount of recovered thyroid hormone from said serum by diluting said serum from about 1.5 to about 6 times its initial volume with a nonionic aqueous dilutent and thereafter extracting said thyroid hormone by adding sufficient organic solvent to the diluted serum to yield a final organic solvent concentration therein of at least about 60 percent by volume.
Claim 13
Dependent Claim
13 words
The method of claim 12 wherein said nonionic aqueous diluent is distilled water.
Claim 14
Dependent Claim
12 words
The method of claim 13 wherein said organic solvent is an alcohol.
Claim 15
Dependent Claim
13 words
The method of claim 14 wherein said organic solvent comprises 90 percent ethanol.
References Cited (Backward Citations)
US3507618A
Cited
US3451777A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-11-26 | 1977-05-28 | 1977-11-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-11-26 | 1981-05-28 | 1981-11-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-11-26 | 1985-05-28 | 1985-11-27 | $7700.00 | Window Closed / Lapsed |