Patent US2422758A successfully refreshed from Google Patents.
US3666854A
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Test for thyroid hormone
Assignee: NUCLEAR MED LAB
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Filing: 1969-07-30
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Granted: 1972-05-30
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Expires: 1989-07-30
Abstract
Abstract A procedure utilized in a diagnostic test for determining the level of thyroid hormone within a body fluid which includes admixing a radioactive isotope labeled hormone with a body fluid, and separating the resulting unbound hormone from the hormone bound by the hormone binding globulin and protein of the fluid by sorption of the unbound hormone by a particulate inorganic crystalline material. This sorption does not depend upon critical time or temperature limitations, and is generally conducted by thoroughly admixing the sorbent material with the solution containing the bound and unbound thyroid hormone at room temperature. The sorbent material is then separated from the resultant supernatant fluid and either the supernatant fluid or sorbent material, preferably the sorbent material, is counted with a scintillation counter to determine the labeled hormone bound to or free from the globulin.
Inventors
- 👤 Anna M Eisentraut
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 04:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
18 words
The improved test of claim 1 wherein the said particulate inorganic crystalline sorbent material comprises colloidal magnesium silicate.
Claim 2
Dependent Claim
25 words
The improved test of claim 2 wherein said sorbing occurs at a temperature within the range of from about 20* C to about 30* C.
Claim 3
Dependent Claim
19 words
The method of claim 3 wherein said sorbing occurs during a time interval of at least about 2 minutes.
Claim 4
Dependent Claim
12 words
The improved test of claim 4 wherein said sample fluid comprises blood.
Claim 5
Independent Claim
155 words
A method for determining the level of thyroid hormone in a sample of body fluid containing an unknown quantity of said hormone and hormone binding globulin comprising: a. thoroughly admixing a solution of this sample with a solution containing a radioactive isotope labeled hormone and barbital and allowing the resulting solution to equilibrate; b. thoroughly admixing the resulting equilibrated solution from step (a) with a particulate inorganic crystalline sorbent material selected from silicic acid and carbonates, phosphates, oxides, hydroxides, silicates, aluminates, and sulfates of the metallic elements in Groups IA, IIA, IIIA, IIB, and VIII of the Periodic Table, and mixed salts thereof to remove free thyroid hormone from said solution by selective sorption thereof by said sorbent material; c. separating said sorbent from said solution; and d. counting with a scintillation counter one of (1) the free radioisotope labeled hormone in said sorbent, and (2) the bound radioisotope labeled hormone in said resulting solution.
Claim 6
Dependent Claim
13 words
The method of claim 7 wherein said sorbent material is colloidal magnesium silicate.
Claim 7
Independent Claim
159 words
A method of measuring thyroxine in a sample solution comprising: a. precipitating proteins from said sample solution by admixing said sample solution with an organic solvent and thereby leaving said thyroxine in solution; b. thoroughly admixing a solution of the thyroxine from step (a) with a solution containing thyrobinding globulin, radioactive isotope labeled thyroxine, and barbital and allowing the resulting solution to equilibrate; C. thoroughly admixing the equilibrated solution of step (b) with a particulate inorganic crystalline sorbent material selected from silicic acid and carbonates, phosphates, oxides, hydroxides, silicates, aluminates, and sulfates of the metallic elements of Groups IA, IIA, IIIA, IIB and VIII of the Periodic Table, and mixed salts thereof to remove free thyroxine therefrom by selective sorption thereof by said sorbent material; d. separating said sorbent material from the resulting solution; and C. counting with a scintillation counter one of (1) free labeled thyroxine removed in said sorbent, and (2) labeled thyroxine remaining in said solution.
Claim 8
Dependent Claim
13 words
The method of claim 8 wherein said sorbent material is colloidal magnesium silicate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-05-30 | 1975-11-29 | 1976-05-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-05-30 | 1979-11-29 | 1980-05-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-05-30 | 1983-11-29 | 1984-05-30 | $7700.00 | Window Closed / Lapsed |