Patent US4088749A successfully refreshed from Google Patents.
US4088749A
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US
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Composition and method for determination of pregnancy
Assignee: Rafa Laboratories Ltd
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Filing: 1976-04-26
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Granted: 1978-05-09
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Expires: 1996-04-26
Abstract
Abstract Composition for the determining of whether or not a female is pregnant are provided. The composition consists essentially of a neutral and inert particle on which gamma -globulin is adsorbed, said gamma -globulin being coupled to a bi-functional coupling agent, the two functional groups thereof possessing different reactivities, the other functional group of said coupling agent being coupled to HCG. The body fluid of the female suspected of being pregnant is mixed with the above composition and permitted to stand. Agglutination indicates non-pregnancy whereas failure of agglutination indicates pregnancy.
Inventors
- 👤 Lea Grundman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 19:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/826; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
127 words
Method of producing a seriodiagnostic composition for determining whether or not a female is pregnant, said composition consisting essentially of solid neutral particles having γ-globulin adsorbed thereon, said γ-globulin being coupled to one functional group of a bi-functional coupling agent having two functional groups with different reactivities, the other of said functional groups being coupled to human chorionic gonadotropin, which comprises linking one of the functional groups of said coupling agent at a temperature of about 0°-10° C and at a pH of 5.4-7.4 to said human chorionic gonadotropin and then linking said coupling agent at the other of its functional groups at a temperature of about 30°-60° C and at a pH of about 7.6-9.2 to said γ-globulin which has been adsorbed on said neutral particles.
Claim 2
Dependent Claim
127 words
Method of producing a seriodiagnostic composition for determining whether or not a female is pregnant, said composition consisting essentially of solid neutral particles having γ-globulin adsorbed thereon, said γ-globulin being coupled to one functional group of a bi-functional coupling agent having two functional groups with different reactivities, the other of said functional groups being coupled to human chorionic gonadotropin, which comprises linking one of the functional groups of said coupling agent at a temperature of about 0°-10° C and at a pH of 5.4-7.4 to said human chorionic gonadotropin and then linking said coupling agent at the other of its functional groups at a temperature of about 30°-60° C and at a pH of about 7.6-9.2 to said γ-globulin which has been adsorbed on said neutral particles.
Claim 3
Dependent Claim
13 words
The method of claim 1, wherein said neutral particles are of polystyrene latex.
Claim 4
Dependent Claim
11 words
The method of claim 1, wherein said coupling agent is toluene-2,4-diisocyanate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-05-08 | 1981-11-07 | 1982-05-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-05-08 | 1985-11-07 | 1986-05-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-05-08 | 1989-11-07 | 1990-05-09 | $7700.00 | Window Closed / Lapsed |