US4070153A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of serum standard for radioassay of thyroxine
Assignee: Corning Glass Works
•
Filing: 1976-06-18
•
Granted: 1978-01-24
•
Expires: 1996-06-18
Abstract
Abstract Thyroxine binding globulin (TBG) and thyroxine (T4) can be efficiently removed fron serum containing TBG and T4 by contacting the serum with finely-divided alumina particles to adsorb a major portion of the T4 and then reacting the serum at an acidic pH with an anion exchange resin to extract substantially all TBG and the remaining T4.
Inventors
- 👤 Karen L. Travis
- 👤 Frank B. Ward
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 13:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/106664; Y10T436/107497
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
68 words
A method of preparing a serum standard useful for radioassays of thyroxine, the method comprising: contacting a human serum containing thyroxine binding globulin and thyroxine with finely-divided alumina particles to remove a major portion of the thyroxine, and then reacting the serum at an acidic pH with an anion exchange resin for a period of time sufficient to extract substantially all thyroxine binding globulin and the remaining thyroxine.
Claim 2
Independent Claim
68 words
A method of preparing a serum standard useful for radioassays of thyroxine, the method comprising: contacting a human serum containing thyroxine binding globulin and thyroxine with finely-divided alumina particles to remove a major portion of the thyroxine, and then reacting the serum at an acidic pH with an anion exchange resin for a period of time sufficient to extract substantially all thyroxine binding globulin and the remaining thyroxine.
Claim 3
Dependent Claim
20 words
The method of claim 1 wherein the weight of the alumina is at least about 10% of the serum weight.
Claim 4
Dependent Claim
18 words
The method of claim 1 wherein the reaction with the resin is at a pH of about 4.0.
Claim 5
Dependent Claim
17 words
A method of claim 1 wherein the alumina particles have a particle size of about 30-45 mesh.
Claim 6
Dependent Claim
43 words
The method of claim 1 wherein the weight of the alumina particles is at least about 10% of the serum weight, the particles have a particle size of 30-45 mesh, and the reaction with the resin is at a pH of about 4.0.
Claim 7
Dependent Claim
18 words
The method of claim 5 wherein the total reaction time of both steps is less than 8 hours.
Claim 8
Dependent Claim
14 words
The method of claim 6 wherein the total reaction time is about 6 hours.
References Cited (Backward Citations)
US3666854A
Cited
US3743482A
Cited
US3775615A
Cited
US3947564A
Cited
US4005187A
Cited
US3962039A
Cited
US3970746A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-23 | 1981-07-25 | 1982-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-23 | 1985-07-25 | 1986-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-23 | 1989-07-25 | 1990-01-24 | $7700.00 | Window Closed / Lapsed |