US4045384A
Expired / Lapsed
US
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Method for forming an amide bond between a latex and protein
Assignee: Dow Chemical Co
•
Filing: 1976-07-23
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Granted: 1977-08-30
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Expires: 1996-07-23
Abstract
Abstract A method of linking a protein to a carboxylated latex by an amide bond which comprises the steps of forming an active ester latex, dialyzing the active ester latex, and coupling the latex to a protein such as an antibody or an antigen.
Inventors
- 👤 Linneaus C. Dorman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:49
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/76; A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; C; C08; C08G; C08G81/00; C08G81/02; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
54 words
A method for coupling a carboxylated latex to a protein with an amide bond which comprises reacting the latex with a water soluble carbodiimide and a water solubilized N-hydroxy compound to form an active ester latex, removing unchanged reactants from the active ester latex and coupling the cleaned active ester latex with a protein.
Claim 2
Independent Claim
54 words
A method for coupling a carboxylated latex to a protein with an amide bond which comprises reacting the latex with a water soluble carbodiimide and a water solubilized N-hydroxy compound to form an active ester latex, removing unchanged reactants from the active ester latex and coupling the cleaned active ester latex with a protein.
Claim 3
Dependent Claim
35 words
The method of claim 1 wherein the latex is a monodispersed uniform particle size latex selected from the group consisting of polymers ad copolymers containing acrylic acid, itaconic acid, fumaric acid, styrene, and methacrylic acid.
Claim 4
Dependent Claim
47 words
The method of claim 2 wherein the latex is reacted with an amount of the water-soluble carbodiimide equal to the chemical equivalence of the carboxyl groups of the latex for a period of from 3 to 5 hours at a temperature of from 2° to 5° C.
Claim 5
Dependent Claim
11 words
The method of claim 3 wherein the latex is styrene-acrylamide succinylhydrazide.
Claim 6
Dependent Claim
11 words
The method of claim 3 wherein the N-hydroxy compound is N-hydroxybenzotriazole.
Claim 7
Dependent Claim
12 words
The method of claim 4 wherein the protein is human chorionic gonadotropin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-29 | 1981-02-28 | 1981-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-29 | 1985-02-28 | 1985-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-29 | 1989-02-28 | 1989-08-30 | $7700.00 | Window Closed / Lapsed |