Patent EP0328256A1 successfully refreshed from Google Patents.
EP0328256A1
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Glass fibers coated with agarose for use as column packing or chromatographic media for bioseparations
Assignee: Owens Corning
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Filing: 1989-01-19
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Granted: —
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Expires: 2009-01-19
Abstract
Abstract The invention discloses a glass fiber coated with a porous hydrophilic matrix material comprised of agarose which is derivatized to bind a suitable ligand or biological material. The glass fibers of the present invention are useful in removing contaminates in a chromatographic process. In one embodiment glass fibers have on their surface a coating comprising agarose and polymyxin B which coated glass fibers are useful for removing endotoxins in a bioseparation process.
Inventors
- 👤 Gary Masaru Nishioka
- 👤 Mary Beth Henderson
- 👤 William Edward Downey
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 20:08
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3274; B01J20/281; B01J20/286; B01J20/3202; B01J20/3204; B01J20/327; B01J20/328; B01J20/3291; B01J20/3293; C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K17/00; C07K17/14; C12; C12N; C12N11/00; C12N11/14
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
23 words
A glass fiber having on its surface a coating comprising a porous hydrophilic matrix derivatized to bind a suitable ligand or biological material.
Claim 2
Dependent Claim
23 words
A glass fiber having on its surface a coating comprising a porous hydrophilic matrix derivatized to bind a suitable ligand or biological material.
Claim 3
Dependent Claim
21 words
A glass fiber according to claim 1, wherein the porous hydrophilic matrix is selected from agarose, polyacrylamides, dextran and hydroxyalkyl methacrylates.
Claim 4
Dependent Claim
22 words
A column packing or chromatographic medium useful for bioseparations comprising a plurality of glass fibers according to claim 1 or claim 2.
Claim 5
Dependent Claim
22 words
A biological material composite comprising a ligand or biological material immobilized on a glass fiber according to claim 1 or claim 2.
Claim 6
Dependent Claim
26 words
A glass fiber having on its surface a coating comprising a porous hydrophilic matrix comprised of agarose derivatized to bind a suitable ligand or biological material.
Claim 7
Dependent Claim
26 words
A glass fiber having on its surface a coating comprising a porous hydrophilic matrix comprised of agarose derivatized to bind a suitable ligand or biological material.
Claim 8
Dependent Claim
22 words
A glass fiber according to claim 5, wherein the agarose matrix is present in an amount of at least 1% by weight.
Claim 9
Dependent Claim
29 words
A glass fiber according to claim 5 or claim 6, wherein the agarose matrix has been applied to the glass fiber surface in the form of a hot melt.
Claim 10
Dependent Claim
29 words
A glass fiber according to any one of claims 5 to 7, wherein the agarose matrix has been applied to the glass fiber surface using a coating off-line process.
Claim 11
Dependent Claim
25 words
A glass fiber according to any one of claims 5 to 8, wherein the agarose matrix has been activated with cyanogen bromide or sulfonyl chloride.
Claim 12
Dependent Claim
29 words
A column packing or chromatographic medium useful for removing toxins in a bioseparation process comprising a plurality of glass fibers according to any one of claims 5 to 9.
Claim 13
Dependent Claim
24 words
A biological material composite comprising a ligand or biological material immobilized on a glass fiber according to any one of claims 5 to 9.
Claim 14
Dependent Claim
36 words
A composite according to claim 4 or claim 11, wherein the ligand or biological material is selected from animal cells, antibodies, antibiotics, antigens, carbohydrates, bacteria, coenzymes, enzymes, fungi, microbial cells, plant cells, tissue cultures and yeast.
Claim 15
Dependent Claim
15 words
A composite according to claim 12, wherein the ligand or biological material comprises an antibiotic.
Claim 16
Dependent Claim
12 words
A composite according to claim 13, wherein the antibiotic comprises polymyxin B.
References Cited (Backward Citations)
FR2083067A5
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US4111838A
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EP0197784A1
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EP0203049A1
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WO1987007531A1
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EP0266580A2
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