EP0197784A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for immobilizing a biological material
Assignee: Genex Corp
•
Filing: 1986-04-04
•
Granted: —
•
Expires: 2006-04-04
Abstract
Abstract The present invention provides a method for immobilizing a biological material by preparing an insolubilized biological material composite comprising the steps of: (a) coating glass fibers of matrices with a mixture of the biological material and a polymer; and (b) insolubilizing the polymer by applying a cross-linking agent and/or a condensing agent to the coated glass fibers or matrices.
Inventors
- 👤 Wayne E. Swann
- 👤 Carol A. Nolf
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 19:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/098; C12N11/089; C12N11/14; C12P; C12P13/00; C12P13/04; C12P13/20; C12P13/22; C12P13/222; C12P19/00; C12P19/20; G01N33/551; G01N33/552
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
55 words
A method for immobilizing a biological material by preparing an insolubilized biological material composite comprising the steps of: (a) coating glass fibers of matrices with a mixture of the biological material and a polymer; and (b) insolubilizing the polymer by applying a cross-linking agent and/or a condensing agent to the coated glass fibers or matrices.
Claim 2
Independent Claim
55 words
A method for immobilizing a biological material by preparing an insolubilized biological material composite comprising the steps of: (a) coating glass fibers of matrices with a mixture of the biological material and a polymer; and (b) insolubilizing the polymer by applying a cross-linking agent and/or a condensing agent to the coated glass fibers or matrices.
Claim 3
Dependent Claim
30 words
A method as claimed in claim 1 wherein the mixture of the biological material and the polymer employed to coat the glass fibers or matrices is in an aqueous medium.
Claim 4
Dependent Claim
27 words
A method as claimed in claim 1 or claim 2 wherein the polymer is selected from the group consisting of polyalkyleneimines, polysaccharides, polyacrylamide, polyurethane, alginate and carageenan.
Claim 5
Dependent Claim
13 words
A method as claimed in claim 3 wherein the polymer is a polyalkyleneimine.
Claim 6
Dependent Claim
24 words
A method as claimed in claim 4 wherein the polyalkyleneimine polymer is selected from the group consisting of polyethyleneimine, polypropyl- eneimine, polybutyleneimine and polypentyleneimine.
Claim 7
Dependent Claim
12 words
A method as claimed in claim 5 wherein the polymer is polyethyleneimine.
Claim 8
Independent Claim
35 words
A method as claimed in any one of claims 4 to 6 wherein a cross-linking amount of an amine cross-linking agent is applied to said coated glass fibers or matrices to immobilize the biological material.
Claim 9
Independent Claim
65 words
A method as claimed in any one of claims 4 to 6 wherein said polvalkyleneimine polvmer is mixed with a polycarboxylic acid to produce a water soluble pre-polymer before it is coated onto said glass fibers or matrices and the said water soluble pre-polymer after application to the glass fibers or matrices is condensed by adding a condensing amount of a carbodiimide under condensing conditions.
Claim 10
Dependent Claim
32 words
A method as claimed in claim 8 further comprising modifying the insolubilized biological material composite by post-treatment with an amine cross- linking agent to impart additional strength and stability to the composite.
Claim 11
Dependent Claim
62 words
A method as claimed in claim 8 or claim 9 wherein the polycarboxylic acid is selected from the group consisting of adipic acid, azelaic acid, 1,11-undecanedioic acid, 1,12-dodecanedioic acid, traumtic acid, pentadecanedioic acid, hexadecanedioic acid, sebacic acid, suberic acid, glutaric acid, malonic acid, pimellic acid, succinic acid, malic acid, maleic acid, glutamic acid, aspartic acid, oxalic acid, fumaric acid and polyaspartic acid.
Claim 12
Independent Claim
33 words
A method as claimed in any one of claims 8 to 10 wherein the carbodiimide is selected from the group consisting of 1-ethyl-3,3-dimethylaminopropyl carbodiimide, dicyclohexyl carbodiimide and 1-cyclohexyl 3-(2-morpholinoethyl)carbodiimidemetho-p-toluene sulphonate and salts thereof.
Claim 13
Dependent Claim
29 words
A method as claimed in claim 7 wherein said amine cross-linking agent is selected from the group consisting of glutaraldehyde, diisocyanates, polyisocyanates, 2,4,6-trichloro-S-triazine, bisoxirane, bissimidate, divinyl-sulfone, 1,5-difluoro-2,4-dinitrobenzene and epoxides.
Claim 14
Dependent Claim
26 words
An insolubilized biological material composite comprising glass fibers or matrices having on the surface thereof a mixture of a biologically active material and a cross-linked polymer.
Claim 15
Dependent Claim
26 words
An insolubilized biological material composite comprising glass fibers or matrices having on the surface thereof a mixture of a biologically active material and a cross-linked polymer.
Claim 16
Dependent Claim
19 words
An insolubilized biological material composite as claimed in claim 13 prepared by a method as claimed in claim 7.
Claim 17
Dependent Claim
19 words
An insolubilized biological material composite as claimed in claim 13 prepared by a method as claimed in claim 8.
Claim 18
Dependent Claim
23 words
A composite as claimed in any one of claims 13 to 15 comprising glass fibers having a diameter between 2 and 20 microns.
Claim 19
Dependent Claim
41 words
A composite as claimed in any one of claims 13 to 16 wherein the biologically active material is selected from the group consisting of enzymes, microbial cells, antigens, antibodies, antibiotics, co-enzymes, bacteria, yeast, fungi, plant cells, animal cells and tissue cultures.
Claim 20
Dependent Claim
14 words
A composite as claimed in claim 17 wherein the biologically active material is glucoamylase.
Claim 21
Dependent Claim
18 words
A composite as claimed in claim 17 wherein the biologically active material is aspartase or aspartase-containing microbial cells.
Claim 22
Dependent Claim
21 words
A composite as claimed in claim 17 wherein the biologically active material is an alkaline protease-producing microorganism of the genus Bacillus.
Claim 23
Dependent Claim
22 words
A composite as claimed in claim 17 wherein the biologically active material is phenylalanine ammonia lyase or phenylalanine ammonia lvase-containing microbial cells.
Claim 24
Dependent Claim
30 words
A method for making aspartic acid which comprises contacting, under asoartic acid-producing conditions, a substrate solution containing ammonium fumarate with an insolubilized biological material composite as claimed in claim 19.
Claim 25
Dependent Claim
29 words
A method for making an alkaline protease which comprises contacting, under alkaline protease-producing conditions, a suitable fermentation medium with an insolubilized biological material composite as claimed in claim 20.
Claim 26
Dependent Claim
28 words
A method for making L-phenylalanine which comprises contacting, under L-phenylalanine-producing conditions, a substrate solution containing ammonium cinnamate with an insolubilized biological material composite as claimed in claim 21.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.