Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
86 words
Process for the production of ethanol and a protein or peptide which is heterologous to yeast which comprises fermenting an aqueous .carbohydrate-containing medium with a yeast strain which has been genetically modified to be capable of expressing a heterologous protein or peptide under conditions such that the yeast multiplies but no expression of the said heterologous protein or peptide takes place, recovering the ethanol so formed, inducing expression of the said protein or peptide by the yeast, and obtaining the said heterologous protein or peptide therefrom.
Claim 2
Dependent Claim
86 words
Process for the production of ethanol and a protein or peptide which is heterologous to yeast which comprises fermenting an aqueous .carbohydrate-containing medium with a yeast strain which has been genetically modified to be capable of expressing a heterologous protein or peptide under conditions such that the yeast multiplies but no expression of the said heterologous protein or peptide takes place, recovering the ethanol so formed, inducing expression of the said protein or peptide by the yeast, and obtaining the said heterologous protein or peptide therefrom.
Claim 3
Dependent Claim
37 words
Process according to claim 1 in which the said yeast contains a plasmid having the GAL 10-CYCl hybrid promoter and expression of the said protein or peptide is induced by exposing the said multiplied yeast to galactose.
Claim 4
Dependent Claim
24 words
Protein according to claim 1 or 2 in which the said protein or peptide is human serum albumin or a human serum albumin-like protein.
Claim 5
Dependent Claim
43 words
Process according to any of claims 1 to 3 in which the ethanol is recovered in the form of an aqueous potable liquid which is substantially free from yeast and which contains substantially all the water and ethanol of the said fermented medium.
Claim 6
Dependent Claim
27 words
Process according to any of claims 1 to 3 in which the ethanol is recovered from the said fermented medium in the form of an ethanolic distillate.
Claim 7
Dependent Claim
18 words
Process according to claim 4 in which the aqueous carbohydrate-containing medium contains maltose as the major sugar present.
Claim 8
Dependent Claim
17 words
Process according to claim 6 in which the aqueous carbohydrate-containing medium is a barley malt-based beer wort.
Claim 9
Dependent Claim
20 words
Process according to claim 4, 6 or 7 in which the fermentation is effected at 8 to 25 °C .
Claim 10
Dependent Claim
25 words
Process according to claim 5 in which the aqueous carbohydrate-containing medium is a fermentation medium for the production of potable distilled ethanol or power ethanol.
Claim 11
Dependent Claim
35 words
Process according to claim 9 in which the said medium is based on grain, potatoes, cassava, sugar cane, sugar beet or a lignocellulosic material optionally pretreated to convert cellulose and/or starch therein into fermentable. sugars.
Claim 12
Dependent Claim
19 words
Process according to any one of claims 1 to 10 in which the fermentation is a substantially anaerobic fermentation.
Claim 13
Dependent Claim
30 words
Process according to any one of claims 1 to 11 in which the yeast used is a genetically engineered modification of an industrial strain of Saccharromyces cerevisiae, or S. carlsbergensis.