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EP0147198A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Fermentation processes and their products

Assignee: Albumedix Ltd
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Filing: 1984-12-20
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Granted: —
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Expires: 2004-12-20
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Abstract

Abstract The invention provides a process for the production of ethanol and a protein or peptide which is heterologous to yeast which comprises fermenting an aqueous sugar-containing medium with an industrial yeast strain which has been genetically modified to be capable of expressing a heterologous protein or peptide, recovering the ethanol so-formed, and obtaining the said heterologous protein or peptide from the fermentation products. The process may be applied to the industrial production of alcoholic beverages such as beer or distilled alcohol. The yeast inevitably obtained as a by-product in the process has improved value because of the heterologous protein or peptide which it contains and provides a source of the latter. Heterologous protein and peptides which may be produced by the new process include enzymes such as beta-lactamase, beta-glucanase and beta-galactosidase and proteins of therapeutic value such as human serum albumen.

Inventors

  • 👤 Stuart William Molzahn

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-26 21:05
CPC Classifications
C; C12; C12Y; C12Y302/00; C12Y302/01; C12Y302/01073; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2468; C12N9/2471; C12N9/78; C12N9/86; C12P; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12Y302/01023; C12Y305/00; C12Y305/02; C12Y305/02006; Y; Y02; Y02E; Y02E50/00; Y02E50/10

Claims Analysis

Total Claims: 12
Claim 1
Independent Claim 59 words
Process for the production of ethanol and a protein or peptide which is heterologous to yeast which comprises fermenting an aqueous sugar-containing medium with a yeast strain which has been genetically modified to be capable of expressing a heterologous protein or peptide, recovering the ethanol so formed, and obtaining the said heterologous protein or peptide from the fermentation products.
Claim 2
Dependent Claim 59 words
Process for the production of ethanol and a protein or peptide which is heterologous to yeast which comprises fermenting an aqueous sugar-containing medium with a yeast strain which has been genetically modified to be capable of expressing a heterologous protein or peptide, recovering the ethanol so formed, and obtaining the said heterologous protein or peptide from the fermentation products.
Claim 3
Dependent Claim 47 words
Process according to claim 1 in which the ethanol is recovered in the form of an aqueous potable liquid which is substantially free from yeast and from the said heterologous protein or peptide and which contains substantially all the water and ethanol of the said fermented medium.
Claim 4
Dependent Claim 23 words
Process according to claim 1 in which the ethanol is recovered from the said fermented medium in the form of an ethanolic distillate.
Claim 5
Dependent Claim 18 words
Process according to claim 2 in which the aqueous sugar-containing medium contains maltose as the major sugar present.
Claim 6
Dependent Claim 17 words
Process according to claim 4 in which the aqueous sugar-containing medium is a barley malt-based beer wort.
Claim 7
Dependent Claim 18 words
Process according to claim 2, 4 or 5 in which the fermentation is effected at 8 to 25°C.
Claim 8
Dependent Claim 25 words
Process according to claim 3 in which the aqueous sugar-containing medium is a fermentation medium for the production of potable distilled ethanol or power ethanol.
Claim 9
Dependent Claim 32 words
Process according to claim 7 in which the said medium is based on grain, potatoes, cassava, sugar cane, or sugar beet, optionally pretreated to convert cellulose and/or starch therein into fermentable sugars.
Claim 10
Dependent Claim 19 words
Process according to any one of claims 1 to 8 in which the fermentation is a substantially anaerobic fermentation.
Claim 11
Dependent Claim 30 words
Process according to any one of claims 1 to 9 in which the yeast used is a genetically engineered modification of an industrial strain of Saccharromyces cerevisiae, or S. carlsbergensis.
Claim 12
Dependent Claim 31 words
Process according to any of claims 1 to 10 in which the said heterologous protein or peptide is obtained as protein or peptide retained in the yeast produced during the fermentation.

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