EP0079739A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Albumin-based nucleotides, their replication and use, and plasmids for use therein
Assignee: Pharmacia and Upjohn Co
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Filing: 1982-11-08
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Granted: —
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Expires: 2002-11-08
Abstract
Abstract The DNA sequence coding for human serum albumin has been isolated and inserted as two fragments into two novel plasmids which can be replicated in E. coli. These novel fragments can be joined to provide a unitary DNA sequence which then can be cloned into a suitable host, e.g. E. coli, for the expression of human serum albumin (which is used extensively in medical practice in treating shock conditions).
Inventors
- 👤 Achilles Dugaiczyk
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 14:54
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
12 words
Plasmid pHA36, having a restriction endonuclease pattern as shown in the drawing.
Claim 2
Dependent Claim
12 words
Plasmid pHA36, having a restriction endonuclease pattern as shown in the drawing.
Claim 3
Dependent Claim
12 words
Plasmid pHA206, having a restriction endonuclease pattern as shown in the drawing.
Claim 4
Dependent Claim
12 words
Plasmid pHA206, having a restriction endonuclease pattern as shown in the drawing.
Claim 5
Dependent Claim
11 words
E. coli HB101 (pHA36) having the deposit accession number NRRL B-12551.
Claim 6
Dependent Claim
11 words
E. coli HB101 (pHA36) having the deposit accession number NRRL B-12551.
Claim 7
Dependent Claim
11 words
E. coli HB101 (pHA206) having the deposit accession number NRRL B-12550.
Claim 8
Dependent Claim
11 words
E. coli HB101 (pHA206) having the deposit accession number NRRL B-12550.
Claim 9
Dependent Claim
24 words
A microorganism modified to contain a nucleotide sequence coding for the amino acid sequence of human serum albumin; said nucleotide sequence is as follows:
Claim 10
Dependent Claim
24 words
A microorganism modified to contain a nucleotide sequence coding for the amino acid sequence of human serum albumin; said nucleotide sequence is as follows:
Claim 11
Dependent Claim
15 words
Nucleotide sequence of the cDNA of human serum albumin, said nucleotide sequence is as follows:
Claim 12
Dependent Claim
15 words
Nucleotide sequence of the cDNA of human serum albumin, said nucleotide sequence is as follows:
Claim 13
Dependent Claim
16 words
Nucleotide sequence coding for the prepeptide of human serum albumin, said nucleotide sequence is as follows:
Claim 14
Dependent Claim
16 words
Nucleotide sequence coding for the prepeptide of human serum albumin, said nucleotide sequence is as follows:
Claim 15
Dependent Claim
14 words
Nucleotide sequence coding for pro human serum albumin, said nucleotide sequence is as follows:
Claim 16
Dependent Claim
14 words
Nucleotide sequence coding for pro human serum albumin, said nucleotide sequence is as follows:
Claim 17
Dependent Claim
16 words
Nucleotide sequence coding for the pre pro human serum albumin, said nucleotide sequence is as follows:
Claim 18
Dependent Claim
16 words
Nucleotide sequence coding for the pre pro human serum albumin, said nucleotide sequence is as follows:
Claim 19
Dependent Claim
18 words
1 0 . A nucleotide sequence according to any of claims 6 to 9, in essentially pure form.
Claim 20
Dependent Claim
13 words
A DNA transfer vector comprising a nucleotide sequence as defined in claim 5.
Claim 21
Dependent Claim
15 words
A DNA transfer vector according to claim 11, transferred to and replicated in a micro-organism.
Claim 22
Dependent Claim
12 words
A DNA transfer vector according to claim 12, which is a plasmid.
Claim 23
Dependent Claim
15 words
A DNA transfer vector according to claim 13, wherein the plasmid is pBR322 or YEp6.
Claim 24
Dependent Claim
16 words
A process for preparing human serum albumin, which comprises culturing a micro-organism according to claim 5.
Claim 25
Dependent Claim
27 words
A DNA transfer vector according to any of claims 12 to 14, or a process according to claim 15, wherein the micro-organism is a bacterium or yeast.
Claim 26
Dependent Claim
19 words
A vector or process according to claim 16, wherein the bacterium or yeast is E. coli or Saccharomyces cerevisiae.
References Cited (Backward Citations)
No citation records indexed.
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