Patent EP0200381A1 successfully refreshed from Google Patents.
EP0200381A1
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A solid phase system for use in ligand-receptor assays
Assignee: Hybritech Inc
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Filing: 1986-04-04
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Granted: —
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Expires: 2006-04-04
Abstract
Abstract A solid phase system for use in a ligand-receptor assay for the detection of a selected analyte in a fluid sample, comprising a porous matrix in which microspheres are entrapped wherein said microspheres are bound with a receptor capable of capturing a target ligand.
Inventors
- 👤 Albert Samuel Rubenstein
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 20:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G01N33/54313; G01N33/5436; G01N33/544; G01N33/549; G01N33/569; G01N33/56911; G01N33/56983; G01N33/573; G01N33/576; G01N33/74
Claims Analysis
Total Claims: 55
Claim 1
Independent Claim
46 words
1 . A solid phase system for use in a ligand-receptor assay for the detection of a selected analyte in a fluid sample, comprising a porous matrix in which microspheres are entrapped wherein said microspheres Are bound with a receptor capable of capturing a target ligand.
Claim 2
Dependent Claim
46 words
1 . A solid phase system for use in a ligand-receptor assay for the detection of a selected analyte in a fluid sample, comprising a porous matrix in which microspheres are entrapped wherein said microspheres Are bound with a receptor capable of capturing a target ligand.
Claim 3
Dependent Claim
33 words
The solid phase system according to Claim 1 wherein said matrix is a membrane or filter in which microspheres of a size compatible with the pore size of said matrix may be entrapped.
Claim 4
Dependent Claim
29 words
The solid phase system according to Claim 2 wherein said microspheres are selected to have a size which maximizes the effective surface area of said microspheres within said matrix.
Claim 5
Dependent Claim
31 words
The solid phase system according to Claim 2 wherein said microspheres are selected to have a size within the range of from about 0.1 micron to about 50 microns in diameter.
Claim 6
Dependent Claim
31 words
The solid phase system according to Claim 2 wherein said microspheres are selected to have a size within the range of from about 0.1 micron to about 2.0 microns in diameter.
Claim 7
Dependent Claim
40 words
The solid phase system according to Claim 1 wherein said microspheres are entrapped within said matrix in a manner to permit the mobility of said microspheres/within said matrix and inhibit the aggregation or adhesion of said microspheres within said matrix
Claim 8
Dependent Claim
25 words
The solid phase system according to Claim 1 wherein said membrane or filter is of a material selected from glass fibers, nylon or ceramic materials.
Claim 9
Dependent Claim
20 words
The solid phase system according to Claim 1 wherein said microspheres are entrapped within a discrete zone of said matrix.
Claim 10
Dependent Claim
24 words
The solid phase system according to Claim 1 wherein said microspheres are comprised of a polymeric material capable of being bound with said receptor.
Claim 11
Dependent Claim
24 words
10 . The solid phase system according to Claim 9 wherein said polymeric material is selected from latex. polyethylene, polypropylene and polystryrene and copolymersthereof.
Claim 12
Dependent Claim
15 words
The solid phase system according to Claim 1 wherein said ligand-receptor assay is an immunoassay.
Claim 13
Dependent Claim
26 words
The solid phase system according to Claim 11 wherein said receptor bound with said microspheres is an antibody or antigen capable of capturing said target ligand.
Claim 14
Dependent Claim
15 words
The solid phase system according to Claim 12 wherein said antibody is a monoclonal antibody.
Claim 15
Dependent Claim
60 words
The solid phase system for use in a multiple ligand-receptor assay for the simultaneous dection of at least two selected anaytes in a fluid sample comprising a porous matrix in which distinct groups of microspheres are entrapped within discrete zones wherein each distinct group of microspheres is bound with a receptor capable of capturing a different target lig d nd.
Claim 16
Dependent Claim
60 words
The solid phase system for use in a multiple ligand-receptor assay for the simultaneous dection of at least two selected anaytes in a fluid sample comprising a porous matrix in which distinct groups of microspheres are entrapped within discrete zones wherein each distinct group of microspheres is bound with a receptor capable of capturing a different target lig d nd.
Claim 17
Dependent Claim
33 words
The solid phase system according to Claim 14 wherein said matrix is a membrane or filter in which microspheres of a size compatable with the pore size of said matrix may be entrapped.
Claim 18
Dependent Claim
25 words
The solid phase system according to Claim 14 wherein said membrane or filter is of a material selected from glass fibers, nylon or ceramic materials.
Claim 19
Dependent Claim
24 words
The solid phase system according to Claim 14 wherein said microspheres are comprised of a polymeric material capable of being bound with said receptor.
Claim 20
Dependent Claim
22 words
The solid phase system according to Claim 17 latex, wherein said polymeric material is selected from/polyethylene, polypropylene and polystyrene and copolymers thereof.
Claim 21
Dependent Claim
15 words
The solid phase system according to Claim 14 wherein said ligand-receptor assay is an immunoassay.
Claim 22
Dependent Claim
30 words
The solid phase system according to Claim 19 wherein said receptor bound with at least one group of microspheres is an antibody or antigen capable of capturing said target ligand.
Claim 23
Dependent Claim
17 words
2 1. The solid phase system according to Claim 20 wherein said antibody is a monoclonal antibody.
Claim 24
Dependent Claim
40 words
22 . The solid phase system according to Claim 21 wherein said group of microspheres comprises at least two subpopulations of microspheres to which are bound monoclonal anti- bodies selected to bind at non-interfering epitopes of said ' target ligand.
Claim 25
Dependent Claim
86 words
A solid phase system for use in a ligand-receptor assay for the detection of at least one selected analyte in a fluid sample comprising a porous matrix in which distinct groups of microspheres are entrapped within discrete zones wherein: a) at least one group of microspheres is bound with a receptor capable of capturing a target ligand; and b) at least one group of microspheres is bound with said target ligand or other suitable receptor substance as a positive control for the detection of said analyte.
Claim 26
Dependent Claim
86 words
A solid phase system for use in a ligand-receptor assay for the detection of at least one selected analyte in a fluid sample comprising a porous matrix in which distinct groups of microspheres are entrapped within discrete zones wherein: a) at least one group of microspheres is bound with a receptor capable of capturing a target ligand; and b) at least one group of microspheres is bound with said target ligand or other suitable receptor substance as a positive control for the detection of said analyte.
Claim 27
Dependent Claim
91 words
A solid phase system for use in a ligand-receptor assay for the detection of at least one selected analyte in a fluid sample comprising a porous matrix in which distinct groups of microspheres are entrapped within discrete zones wherein: a) at least one group of microspheres is bound with a receptor capable of capturing d target ligand; and b) at least one group of microspheres is bound with a substance incapable of capturing said target ligand, or without a bound component, as a negative control for the detection of said analyte.
Claim 28
Dependent Claim
91 words
A solid phase system for use in a ligand-receptor assay for the detection of at least one selected analyte in a fluid sample comprising a porous matrix in which distinct groups of microspheres are entrapped within discrete zones wherein: a) at least one group of microspheres is bound with a receptor capable of capturing d target ligand; and b) at least one group of microspheres is bound with a substance incapable of capturing said target ligand, or without a bound component, as a negative control for the detection of said analyte.
Claim 29
Dependent Claim
35 words
The solid phase system according to Claims 23 or 24 wherein said matrix is a membrane or filter in which microspheres of a size compatible with the pore size of said matrix may be entrapped.
Claim 30
Dependent Claim
27 words
The solid phase system according to Claims 23 or 24 wherein said membrane or filter is of a material selected from glass fibers, nylon or ceramic materials.
Claim 31
Dependent Claim
26 words
The solid phase system according to Claims 23 or 24 wherein said microspheres are comprised of a polymeric material capable of being bound with said receptor.
Claim 32
Dependent Claim
22 words
The solid phase system according to Claim 27 wherein said polymeric material is selected from/latex, polyethylene, polypropylene and polystyrene and copolymers therecf.
Claim 33
Dependent Claim
17 words
The solid phase system according to Claims 23 or 24 wherein said ligand-receptor assay is an immunoassay.
Claim 34
Dependent Claim
30 words
The solid phase system according to Claim 29 wherein said receptor bound with at least one group of microspheres is an antibody or antigen capable of capturing said target ligand.
Claim 35
Dependent Claim
15 words
The solid phase system according to Claim 30 wherein said antibody is a monoclonal antibody.
Claim 36
Dependent Claim
31 words
The solid phase system according to Claim 30 further comprising a distinct group of microspheres to which is bound the target ligand or other suitable receptor substance as a positive control.
Claim 37
Independent Claim
71 words
An apparatus for use in a ligand-receptor assat for the detection of at least one selected analyte in a fluid sample comprising: a) a first porous member comprising a solid phase system according to Claims 1, 14, 23 or 24; and b) a means, operatively associated with said first porous member, for facilitating the flow of said fluid sample and liquid reagents used in said assay through said first porous member.
Claim 38
Independent Claim
139 words
An apparatus for use in a ligand-receptor assay for the detection of at least one selected analyte in a fluid sample comprising: a) a first porous member comprising a solid phase system according to Claims 1, 14, 23 or 24; and b) a second absorbent member associated with said first member so as to permit the flow of said fluid sample and liquid reagents used in said assay through said first member and into said second member, said second member having a surface over which said first member is placed and having capillaries therethrough in a direction generally transverse to the surface over which said first member is placed, which capillaries are in communication with the pores of said first member so as to draw fluid which has permeated said first member into the capillaries of said second member.
Claim 39
Dependent Claim
13 words
The apparatus according to Claim 33 wherein said ligand-receptor assay is an immunoass'ay.
Claim 40
Dependent Claim
107 words
A ligand-receptor assay process for the detection of at least one selected analyte in a fluid sample comprising: a) introducing said fluid sample suspected of containing a target ligand onto the first porous member of the d pparatus according to Claim 33, which porous member comprises microspheres to which are bound a receptor capable of binding said target ligand; b) adding a solution of receptor conjugate capable of binding with said target ligand, said receptor conjugate being labeled to permit detection, in order to form a complex of said receptor conjugate with said target ligand; c) detecting said receptor conjugate, if any, bound to said porous member.
Claim 41
Dependent Claim
13 words
The process according to Claim 36 wherein said ligand-receptor assay is an immunoassay.
Claim 42
Dependent Claim
29 words
The process according to Claim 36 wherein said·receptor is an allergen, said ligand is an IgE antibody specific for said.allergen and said receptor conjugate is a labeled anti-IgE antibody.
Claim 43
Dependent Claim
21 words
The process according to Claim 36 wherein said analyte is a virus or an antigen or antibody associated with said virus.
Claim 44
Dependent Claim
39 words
A process according to Claim 39 wherein said ligand is selected from the group consisting of Rubella virus, Rota virus, adeno virus, respiratory syncitial virus, HTLV, hepatitis virus, hepatitis/A, hepatitis/B, hepatitis nonA nonB, influenza virus, cytomegalovirus or herpes virus.
Claim 45
Dependent Claim
27 words
The process according to Claim 36 wherein said analyte is a bacterium, fungus or parasite or an antigen or antibody associated with said bacterium, fungus or parasite.
Claim 46
Dependent Claim
33 words
A process according to Claim 41 wherein said analyte is selected from the group consisting of group A and B streptococcus, N eisseria gonorrhea, Trichomonas vaginalis, Candida albicans, Chlamydia trachomatis or Hemophilus influenza.
Claim 47
Dependent Claim
36 words
The process according to Claim 36 wherein said analyte is a physiological marker substance selected from the group consisting of human choriogonadotropin, prostatic acid phosphatase, prostrate-specific antigen, alphafetoprotein, carcinoembryonic antigen, leutinizing hormone or creatine kinase isoenzyme.
Claim 48
Dependent Claim
17 words
The process according to Claim 36 wherein said sample is serum, plasma, urine or other biological substance.
Claim 49
Dependent Claim
48 words
The process according to Claim 36 wherein said receptor conjugate is labeled with an enzyme and said detecting step includes addition of a suitable substrate to said first porous member to generate a color change upon reaction with said enzyme which is determined by visual or instrumental means.
Claim 50
Dependent Claim
40 words
46 . The process according to Claim 36 wherein said receptor is a monoclonal antibody and said receptor conjugate is a labeled monoclonal antibody, said receptor and said receptor conjugate being selected to bind at non-interfering epitopes of said ligand.
Claim 51
Dependent Claim
39 words
The solid phase system according to Claim 1 wherein said assay is a nucleic acid probe assay and wherein said receptor comprises a nucleic acid sequence complementary to a portion of the nucleic acid sequence of said target ligand.
Claim 52
Dependent Claim
16 words
The apparatus according to Claim 33 wherein said ligand-receptor assay is a nucleic acid probe assay.
Claim 53
Dependent Claim
16 words
The process according to Claim 36 wherein said ligand-receptor assay is a nucleic acid probe assay.
Claim 54
Dependent Claim
50 words
The process according to Claim 36 wherein said receptor is a nucleic acid sequence complementary to a portion of the nucleic acid sequence of said target ligand and said receptor conjugate is a labeled nucleic acid probe complementary to another portion of the nucleic acid sequence of said target ligand.
Claim 55
Dependent Claim
18 words
The solid phase systems according to claims 10, 18 or 28 wherein said microspheres are comprised of latex.
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