Patent EP0126230A1 successfully refreshed from Google Patents.
EP0126230A1
Expired / Lapsed
EP
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Novel DNA and use thereof
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1984-03-14
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Granted: —
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Expires: 2004-03-14
Abstract
Abstract Human immune interferon protein can be obtained by growing a transformant containing a recombinant DNA having a structural gene of human immune interferon downstream from a rec A promoter or promoters and recovering the human immune interferon protein accumulated in the culture broth.
Inventors
- 👤 Masakazu Kikuchi
- 👤 Kyozo Tsukamoto
- 👤 Tsutomu Kurokawa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 12:34
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/57
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
20 words
A recombinant DNA which contains a structural gene of human immune interferon downstream from a rec A promoter or promoters.
Claim 2
Dependent Claim
20 words
A recombinant DNA which contains a structural gene of human immune interferon downstream from a rec A promoter or promoters.
Claim 3
Dependent Claim
34 words
A DNA according to Claim 1 which does not have any other translational initiation codon than that for a structural gene of human immune interferon between the rec A promoter and the structural gene.
Claim 4
Dependent Claim
14 words
A DNA according to Claim 1 or 2, which has one rec A promoter.
Claim 5
Dependent Claim
16 words
A DNA according to Claim 1 or 2, which has a plurality of rec A promoters.
Claim 6
Dependent Claim
19 words
A DNA according to Claim 4, which does not have any translational initiation codon between the rec A promoters.
Claim 7
Dependent Claim
22 words
A DNA according to Claim 1, wherein the structural gene of human immune interferon is of the base sequence of the formula:
Claim 8
Independent Claim
49 words
A method of producing a recombinant DNA containing a structural gene of human immune interferon downstream from a rec A promoter or promoters, which comprises combining a rec A promoter or promoters with a structural gene of human immune interferon drownstream from said promoter or promoters in a plasmid.
Claim 9
Independent Claim
49 words
A method of producing a recombinant DNA containing a structural gene of human immune interferon downstream from a rec A promoter or promoters, which comprises combining a rec A promoter or promoters with a structural gene of human immune interferon drownstream from said promoter or promoters in a plasmid.
Claim 10
Dependent Claim
23 words
A transformant which contains a recombinant DNA having a structural gene of human immune interferon downstream from a rec A promoter or promoters.
Claim 11
Dependent Claim
23 words
A transformant which contains a recombinant DNA having a structural gene of human immune interferon downstream from a rec A promoter or promoters.
Claim 12
Dependent Claim
10 words
A transformant according to Claim 8, which is Escherichia coli.
Claim 13
Dependent Claim
21 words
A transformant according to Claim 9, which is Escherichia coli, of which repressing function against rec A gene expression is lessened.
Claim 14
Independent Claim
36 words
A method of producing a transformant containing a recombinant DNA having a structural gene of human immune interferon downstream from a rec A promoter or promoters, which comprises transforming a host organism with said recombinant DNA.
Claim 15
Independent Claim
36 words
A method of producing a transformant containing a recombinant DNA having a structural gene of human immune interferon downstream from a rec A promoter or promoters, which comprises transforming a host organism with said recombinant DNA.
Claim 16
Dependent Claim
13 words
A method according to Claim 11, wherein the host organism is Escherichia coli.
Claim 17
Independent Claim
45 words
A method of producing human immune interferon protein which comprises growing a transformant containing a recombinant DNA having a structural gene of human immune interferon downstream from a rec A promoter or promoters and recovering the human immune interferon protein accumulated in the culture broth.
Claim 18
Independent Claim
45 words
A method of producing human immune interferon protein which comprises growing a transformant containing a recombinant DNA having a structural gene of human immune interferon downstream from a rec A promoter or promoters and recovering the human immune interferon protein accumulated in the culture broth.
Claim 19
Dependent Claim
12 words
A method according to Claim 13, wherein the transformant is Escherichia coli.
References Cited (Backward Citations)
EP0068719A2
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EP0089676A2
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