EP0089676A2
Expired / Lapsed
EP
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Novel DNA and use thereof
Assignee: Takeda Pharmaceutical Co Ltd
•
Filing: 1983-03-22
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Granted: —
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Expires: 2003-03-22
Abstract
Abstract Human immune interferon is produced by preparing a DNA which contains a base sequence coding for the human immune interferon polypeptide, transforming a host therewith, growing the host and recovering the same.
Inventors
- 👤 Masakazu Kikuchi
- 👤 Kyozo Tsukamoto
- 👤 Tsutomu Kurokawa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 13:17
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/57; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N15/73; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
17 words
A DNA which contains a base sequence of the formula: wherein X is TAA, TGA or TAG.
Claim 2
Dependent Claim
17 words
A DNA which contains a base sequence of the formula: wherein X is TAA, TGA or TAG.
Claim 3
Dependent Claim
16 words
A DNA according to Claim 1, wherein it has at the 5'- end thereof the sequence
Claim 6
Dependent Claim
14 words
A DNA according to Claim 1, wherein it has ATG at the 5'-end thereof.
Claim 7
Dependent Claim
14 words
A DNA according to any of Claims 1-3, wherein it codes for the polypeptide:
Claim 8
Dependent Claim
25 words
A DNA according to any of Claims 1-3, wherein it codes for a polypeptide equivalent to the immune interferon polypeptide in immunological or biological activities.
Claim 9
Dependent Claim
17 words
A D N A according to Claims 1, 3 or 5, wherein it codes for the polypeptide:
Claim 10
Dependent Claim
14 words
A DNA according to Claims 1,2 or 5, wherein it codes for the polypeptide:
Claim 11
Dependent Claim
18 words
A DNA according to any of Claims 1-7, wherein it forms a part of a recombinant DNA molecule.
Claim 12
Dependent Claim
20 words
A DNA according to any of Claims 1-8, wherein it is linked at the site of downstream from a promoter.
Claim 13
Independent Claim
127 words
A method of producing a DNA containing a base sequence of the formula: wherein X is TAA, TGA or TAG, which comprises, (a) growing human immune interferon-secreting cells, (b) isolating from the cells a messenger RNA coding for human immune interferon, (c) synthesizing a single-stranded complementary DNA with the use of said messenger RNA and reverse transcriptase, (d) coverting said complementary DNA to a double-stranded DNA, (e) inserting said double stranded DNA into a plasmid, (f) transforming a host with said recombinant plasmid, (g) isolating a plasmid containing the objective DNA from the culture of said host, (h) if desired, excising out the cloned DNA from the plasmid, and (i) if desired, connecting said cloned DNA at the site of downstream from a promoter in a vehicle.
Claim 14
Independent Claim
127 words
A method of producing a DNA containing a base sequence of the formula: wherein X is TAA, TGA or TAG, which comprises, (a) growing human immune interferon-secreting cells, (b) isolating from the cells a messenger RNA coding for human immune interferon, (c) synthesizing a single-stranded complementary DNA with the use of said messenger RNA and reverse transcriptase, (d) coverting said complementary DNA to a double-stranded DNA, (e) inserting said double stranded DNA into a plasmid, (f) transforming a host with said recombinant plasmid, (g) isolating a plasmid containing the objective DNA from the culture of said host, (h) if desired, excising out the cloned DNA from the plasmid, and (i) if desired, connecting said cloned DNA at the site of downstream from a promoter in a vehicle.
Claim 15
Dependent Claim
14 words
A method according to Claim 10, wherein the objective DNA codes for the polypeptide:
Claim 16
Dependent Claim
25 words
A method according to Claim 10, wherein the objective DNA codes for a polypeptide equivalent to the immune interferon polypeptide in immunological or biological activities.
Claim 17
Independent Claim
18 words
A method according to Claims 10-12, wherein the objective DNA forms a part of a recombinant DNA molecule.
Claim 18
Dependent Claim
20 words
A host transformed with a DNA containing a base sequence of the formula: wherein X is TAA, TGA or TAG.
Claim 19
Dependent Claim
20 words
A host transformed with a DNA containing a base sequence of the formula: wherein X is TAA, TGA or TAG.
Claim 20
Dependent Claim
22 words
A host according to Claim 14, wherein the DNA contains said base sequence linked at the site of downstream from a promoter.
Claim 21
Dependent Claim
17 words
A host according to Claim 14 or 15, wherein said host is Escherichia coli or Bacillus subtilis.
Claim 22
Dependent Claim
33 words
A compound of the formula: wherein Y is (a) hydrogen, (b) Met or (c) Met Lys Tyr Thr Ser Tyr Ile Ala Phe Gln Leu Cys Ile Val Leu Gly Ser Leu Gly.
Claim 23
Dependent Claim
33 words
A compound of the formula: wherein Y is (a) hydrogen, (b) Met or (c) Met Lys Tyr Thr Ser Tyr Ile Ala Phe Gln Leu Cys Ile Val Leu Gly Ser Leu Gly.
Claim 24
Independent Claim
71 words
A method of producing a compound of the formula: wherein Y is (a) hydrogen, (b) Met or (c) Met Lys Tyr Thr Ser Tyr Ile Ala Phe Gln Leu Cys Ile Val Leu Gly Ser Leu Gly, which comprises growing a host transformed with a DNA containing a base sequence of the formula: wherein X is TAA, TGA or TAG, accumulating said compound in the culture broth and recovering the same.
Claim 25
Independent Claim
71 words
A method of producing a compound of the formula: wherein Y is (a) hydrogen, (b) Met or (c) Met Lys Tyr Thr Ser Tyr Ile Ala Phe Gln Leu Cys Ile Val Leu Gly Ser Leu Gly, which comprises growing a host transformed with a DNA containing a base sequence of the formula: wherein X is TAA, TGA or TAG, accumulating said compound in the culture broth and recovering the same.
Claim 26
Dependent Claim
43 words
A sterile pharmaceutical composition, comprising a compound of the formula: wherein Y is (a) hydrogen, (b) Met or (c) Met Lys Tyr Thr Ser Tyr Ile Ala Phe Gln Leu Cys Ile Val Leu Gly Ser Leu Gly, and a pharmaceutically acceptable carrier.
Claim 27
Dependent Claim
43 words
A sterile pharmaceutical composition, comprising a compound of the formula: wherein Y is (a) hydrogen, (b) Met or (c) Met Lys Tyr Thr Ser Tyr Ile Ala Phe Gln Leu Cys Ile Val Leu Gly Ser Leu Gly, and a pharmaceutically acceptable carrier.
Claim 28
Dependent Claim
25 words
A pharmaceutical composition according to Claim 18, wherein Y is hydrogen, and the pharmaceutically acceptable carrier is sterile water, normal saline or human serum albumin.
References Cited (Backward Citations)
No citation records indexed.
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