EP0068719A2
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EP
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Deoxyribonucleic acids, their production and use
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1982-06-16
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Granted: —
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Expires: 2002-06-16
Abstract
Abstract A recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen is constructed. This structural gene is expressed in a transformant containing said recombinant DNA to produce hepatitis B virus subtype adw surface antigen which is useful as a vaccine against hepatitis B.
Inventors
- 👤 Koichi Igarashi
- 👤 Haruo Onda
- 👤 Masakazu Kikuchi
- 👤 Yukio Fujisawa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 13:52
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; A; A61; A61K; A61K39/00; C07K2319/00; C07K2319/40; C07K2319/61; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
16 words
A DNA which contains a structural gene coding for hepatitis B virus subtype adw surface antigen.
Claim 2
Dependent Claim
16 words
A DNA which contains a structural gene coding for hepatitis B virus subtype adw surface antigen.
Claim 3
Dependent Claim
31 words
A DNA according to Claim 1, wherein the structural gene coding for the surface antigen has the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof.
Claim 4
Dependent Claim
33 words
A DNA according to Claim 1 or 2, wherein the structural gene coding for the surface antigen codes for the polypeptide shown.in.Figure 4 or a polypeptide equivalent thereto in immunological or biological activity.
Claim 5
Dependent Claim
37 words
A DNA according to any one of Claims 1-3, which is in the form of a recombinant DNA consisting of a vector DNA and the structural gene coding for the surface antigen inserted in said vector DNA.
Claim 6
Dependent Claim
74 words
A DNA according to any cne of Claims 1-4, which contains the gene coding for the surface antigen as inserted in the gene coding for anthranilic acid synthetase component I or anthranilic acid synthetase component II in the tryptophan operon, the gene coding for rec A protein in the rec A operon or the gene coding for S-lactamase in the ampicillin resistance gene operon, or downstream from the tryptophan promoter or rec A promoter.
Claim 7
Dependent Claim
47 words
A DNA according to any one of Claims 1-5, wherein the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof with ATG joined to the 5'-end without any reading frame shift is inserted downstream from the tryptophan promoter or rec A promoter.
Claim 8
Independent Claim
92 words
A method of producing a recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen, which comprises subjecting a vector DNA and a hepatitis B virus subtype adw DNA from a Dane particle of hepatitis B virus subtype adw, after rendering the single-stranded portion of the latter DNA double-stranded by endogenous DNA synthesis reaction, each to restriction enzyme cleavage, mixing the cleavage products and thereby causing insertion of the hepatitis B virus subtype adw DNA or a fragment thereof at the cleavage site in said vector DNA.
Claim 9
Independent Claim
92 words
A method of producing a recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen, which comprises subjecting a vector DNA and a hepatitis B virus subtype adw DNA from a Dane particle of hepatitis B virus subtype adw, after rendering the single-stranded portion of the latter DNA double-stranded by endogenous DNA synthesis reaction, each to restriction enzyme cleavage, mixing the cleavage products and thereby causing insertion of the hepatitis B virus subtype adw DNA or a fragment thereof at the cleavage site in said vector DNA.
Claim 10
Dependent Claim
31 words
A method according to Claim 7, wherein the structural gene coding for the surface antigen has the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof.
Claim 11
Dependent Claim
35 words
A method according to Claim 7 or 8, wherein the structural gene coding for the surface antigen codes for the polypeptide shown in Figure 4 or a polypeptide equivalent thereto in immunological or biological activity.
Claim 12
Independent Claim
73 words
A method according to any one of Claims 7-9, wherein the gene coding for the surface antigen is inserted in the gene coding for anthranilic acid synthetase component I or anthranilic acid synthetase component II in the tryptophan operon, the gene coding for rec A protein in the rec A operon or the gene coding for S-lactamase in the ampicillin resistance gene operon, or downstream from the tryptophan promoter or rec A promoter.
Claim 13
Independent Claim
47 words
A method according to any one of Claims 7-10 wherein the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof with ATG joined to the 5'-end without any reading frame shift is inserted downstream from the tryptophan promoter or rec A promoter.
Claim 14
Dependent Claim
20 words
A transformant which contains a recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen.
Claim 15
Dependent Claim
20 words
A transformant which contains a recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen.
Claim 16
Dependent Claim
31 words
A transformant according to Claim 12, wherein the structural gene coding for the surface antigen has the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof.
Claim 17
Dependent Claim
35 words
A transformant according to Claim 12 or 13, wherein the structural gene coding for the surface antigen codes for the polypeptide shown in Figure 4 or a polypeptide equivalent thereto in immunological or biolcgical activity.
Claim 18
Dependent Claim
79 words
A transformant according to any one of Claims 12-14 which contains a recombinant DNA containing the structural gene coding for the surface antigen as inserted in the gene coding for anthranilic acid synthetase component I or anthranilic acid synthetase component II in the tryptophan operon, the gene coding for rec A protein in the rec A operon or the gene coding for 3-lactamase in the ampicillin resistance gene operon, or downstream from the tryptophan promoter or rec A promoter.
Claim 19
Dependent Claim
47 words
A transformant according to any one of Claims 12-15, wherein the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof with ATG joined to the 5'-end without any reading frame shift is inserted downstream from the tryptophan promoter or rec A promoter.
Claim 20
Dependent Claim
13 words
A transformant according to any one cf Claims 12-16, which is Escherichia coli.
Claim 21
Independent Claim
86 words
A method of producing hepatitis B virus subtype adw surface antigen or a polypeptide equivalent thereto in immunological or biological activity which comprises growing a transformant with a recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen in an expression system until hepatitis B virus subtype adw surface antigen or a polypeptide equivalent thereto in immunological or biological activity is produced and accumulated in the culture broth, and recovering the accumulated surface antigen or the polypeptide from the culture broth.
Claim 22
Independent Claim
86 words
A method of producing hepatitis B virus subtype adw surface antigen or a polypeptide equivalent thereto in immunological or biological activity which comprises growing a transformant with a recombinant DNA containing a structural gene coding for hepatitis B virus subtype adw surface antigen in an expression system until hepatitis B virus subtype adw surface antigen or a polypeptide equivalent thereto in immunological or biological activity is produced and accumulated in the culture broth, and recovering the accumulated surface antigen or the polypeptide from the culture broth.
Claim 23
Dependent Claim
31 words
A method according to Claim 18, wherein the structural gene coding for the surface antigen has the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof.
Claim 24
Dependent Claim
35 words
A method according to Claim 18 or 19, wherein the structural gene coding for the surface antigen codes for the polypeptide shown in Figure 4 or a polypeptide equivalent thereto in immunological or biological activity.
Claim 25
Independent Claim
81 words
A method according to any one of Claims 18-20, wherein the transformant contains a recombinant DNA containing the structural gene coding for the surface antigen as inserted in the gene coding for anthranilic acid synthetase component I or anthranilic acid synthetase component II in the tryptophan operon, the gene coding for rec A protein in the rec A operon or the gene coding for β-lactamase in the ampicillin resistance gene operon or downstream from the tryptophan promoter or rec A promoter.
Claim 26
Independent Claim
47 words
A method according to any one of Claims 18-21, wherein the base sequence shown in Figure 4 as covering bases 127-807 or a fragment thereof with ATG joined to the 5'-end without any reading frame shift is inserted downstream from the tryptophan promoter or rec A promoter.
Claim 27
Independent Claim
15 words
A method according to any one of Claims 18-22, wherein the transformant is Escherichia coli.
Claim 28
Dependent Claim
11 words
A polypeptide of the amino acid sequence shown in Figure 4.
Claim 29
Dependent Claim
11 words
A polypeptide of the amino acid sequence shown in Figure 4.
Claim 30
Dependent Claim
30 words
Hepatitis B virus subtype adw surface antigen or a polypeptide equivalent thereto in immunological or biological activity, which is obtainable by a method claimed in any one of Claims 18-23.
Claim 31
Dependent Claim
32 words
A composition of matter which comprises hepatitis B virus subtype adw surface antigen or a polypeptide equivalent thereto in immunological or biological activity, the balance of said composition comprising soluble microbial protein.
Claim 32
Dependent Claim
32 words
A composition of matter which comprises hepatitis B virus subtype adw surface antigen or a polypeptide equivalent thereto in immunological or biological activity, the balance of said composition comprising soluble microbial protein.
References Cited (Backward Citations)
No citation records indexed.
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