US7635771B2
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siRNA targeting amyloid beta (A4) precursor protein (APP)
Assignee: Thermo Fisher Scientific Inc
•
Filing: 2007-10-29
•
Granted: 2009-12-22
•
Expires: 2027-10-29
Abstract
Abstract Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for APP.
Inventors
- 👤 Anastasia Khvorova
- 👤 Angela Reynolds
- 👤 Devin Leake
- 👤 William Marshall
- 👤 Steven Read
- 👤 Stephen Scaringe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:30
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; A; A61; A61P; A61P43/00; C12N15/111; C12N15/1135; G; G16; G16B; G16B20/00; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/14; C12N2320/00; C12N2320/10; C12N2320/11
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
86 words
An siRNA molecule, wherein said siRNA molecule consists of: (a) a duplex region; and (b) either no overhangs regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides, wherein the duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region together form said duplex region and said duplex region is 19-30 base pairs in length and said antisense region comprises a sequence that is the complement of SEQ ID NO: 498.
Claim 2
Dependent Claim
86 words
An siRNA molecule, wherein said siRNA molecule consists of: (a) a duplex region; and (b) either no overhangs regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides, wherein the duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region together form said duplex region and said duplex region is 19-30 base pairs in length and said antisense region comprises a sequence that is the complement of SEQ ID NO: 498.
Claim 3
Dependent Claim
21 words
The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 bases in length.
Claim 4
Dependent Claim
21 words
The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 bases in length.
Claim 5
Dependent Claim
17 words
The siRNA molecule of claim 1 , wherein said siRNA molecule has at least one overhang region.
Claim 6
Dependent Claim
15 words
The siRNA molecule of claim 1 , wherein said siRNA molecule has no overhang regions.
Claim 7
Dependent Claim
17 words
The siRNA molecule of claim 2 , wherein said siRNA molecule has at least one overhang region.
Claim 8
Dependent Claim
15 words
The siRNA molecule of claim 2 , wherein said siRNA molecule has no overhang regions.
Claim 9
Dependent Claim
17 words
The siRNA molecule of claim 3 , wherein said siRNA molecule has at least one overhang region.
Claim 10
Dependent Claim
15 words
The siRNA molecule of claim 3 , wherein said siRNA molecule has no overhang regions.
Claim 11
Dependent Claim
42 words
A chemically synthesized double-stranded siRNA molecule, wherein: (a) each strand of said siRNA molecule is between 19 and 30 nucleotides in length; and (b) one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 498.
Claim 12
Dependent Claim
42 words
A chemically synthesized double-stranded siRNA molecule, wherein: (a) each strand of said siRNA molecule is between 19 and 30 nucleotides in length; and (b) one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 498.
Claim 13
Dependent Claim
22 words
The chemically synthesized double-stranded siRNA molecule of claim 10 , wherein each strand of said siRNA molecule is 19 nucleotides in length.
Claim 14
Dependent Claim
148 words
A pool of at least two siRNAs, wherein said pool comprises a first siRNA and a second siRNA, wherein said first siRNA is the siRNA molecule of claim 10 and the duplex region of said first siRNA is a first duplex region and said second siRNA consists of a second duplex region and either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides, wherein the second duplex region of said second siRNA consists of a sense region and an antisense region, wherein said sense region and said antisense region of said second siRNA together form said second duplex region, said second duplex region is 19-30 base pairs in length and said antisense region of said second siRNA comprises a sequence that is the complement of a sequence selected from the group consisting of SEQ ID NO: 438-497 and 499-639.
Claim 15
Dependent Claim
19 words
The pool of claim 12 , wherein said first siRNA and said second siRNA each have no overhang regions.
Claim 16
Dependent Claim
23 words
The pool of claim 12 , wherein said first duplex region and said second duplex region are each 19-25 base pairs in length.
Claim 17
Dependent Claim
23 words
The pool of claim 14 , wherein said first duplex region and said second duplex region are each 19 base pairs in length.
Claim 18
Dependent Claim
14 words
The pool of claim 15 , wherein said first siRNA has no overhang regions.
Claim 19
Dependent Claim
14 words
The pool of claim 16 , wherein said second siRNA has no overhang regions.
Claim 20
Dependent Claim
16 words
The pool of claim 15 , wherein said first siRNA has at least one overhang region.
Claim 21
Dependent Claim
16 words
The pool of claim 18 , wherein said second siRNA has at least one overhang region.
Claim 22
Dependent Claim
76 words
An siRNA molecule consisting of: (a) a duplex region; and (b) either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides, wherein the duplex region is nineteen base pairs in length and consists of a sense region that has a sequence that is the same as SEQ ID NO: 498 and an antisense region that has a sequence that is the complement of SEQ ID NO: 498.
Claim 23
Dependent Claim
76 words
An siRNA molecule consisting of: (a) a duplex region; and (b) either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides, wherein the duplex region is nineteen base pairs in length and consists of a sense region that has a sequence that is the same as SEQ ID NO: 498 and an antisense region that has a sequence that is the complement of SEQ ID NO: 498.
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