US7507811B2
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siRNA targeting KRAS
Assignee: Thermo Fisher Scientific Inc
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Filing: 2006-12-04
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Granted: 2009-03-24
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Expires: 2026-12-04
Abstract
Abstract Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rationale design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes.
Inventors
- 👤 Anastasia Khvorova
- 👤 Angela Reynolds
- 👤 Devin Leake
- 👤 William Marshall
- 👤 Stephen Scaringe
- 👤 Steven Read
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 08:57
CPC Classifications
C; C12; C12Y; C12Y113/00; C12Y113/12; C12Y113/12007; A; A61; A61K; A61K31/00; A61K31/70; A61K31/7088; A61K31/713; A61P; A61P13/00; A61P13/12; A61P21/00; A61P25/00; A61P25/28; A61P3/00; A61P3/08; A61P3/10; A61P35/00; A61P35/02; A61P37/00; A61P37/02; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1034; C12N15/1048; C12N15/11; C12N15/111; C12N15/113; C12N15/1135; C12N15/1136; C12N15/1137; C12N15/1138; C12Y502/00; C12Y502/01; C12Y502/01008; G; G16; G16B; G16B20/00; G16B20/20; G16B20/50; C12N2310/00; C12N2310/10; C12N2310/14; C12N2320/00; C12N2320/10; C12N2320/11
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
66 words
An siRNA molecule, consisting of: (a) a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region consisting of 19-30 base pairs and said antisense region comprises a sequence that is the complement of SEQ. ID. NO. 461; and (b) from zero to four overhang regions, wherein each overhang region is six or fewer nucleotides in length.
Claim 2
Dependent Claim
66 words
An siRNA molecule, consisting of: (a) a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region consisting of 19-30 base pairs and said antisense region comprises a sequence that is the complement of SEQ. ID. NO. 461; and (b) from zero to four overhang regions, wherein each overhang region is six or fewer nucleotides in length.
Claim 3
Dependent Claim
21 words
The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length.
Claim 4
Dependent Claim
21 words
The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 nucleotides in length.
Claim 5
Dependent Claim
21 words
The siRNA molecule of claim 1 , wherein said antisense region is 100% complementary to a region of a target mRNA.
Claim 6
Dependent Claim
17 words
The siRNA molecule of claim 1 , wherein said siRNA molecule has at least one overhang region.
Claim 7
Dependent Claim
15 words
The siRNA molecule of claim 1 , wherein said siRNA molecule has no overhang regions.
Claim 8
Dependent Claim
17 words
The siRNA molecule of claim 2 , wherein said siRNA molecule has at least one overhang region.
Claim 9
Dependent Claim
15 words
The siRNA molecule of claim 2 , wherein said siRNA molecule has no overhang regions.
Claim 10
Dependent Claim
17 words
The siRNA molecule of claim 3 , wherein said siRNA molecule has at least one overhang region.
Claim 11
Dependent Claim
15 words
The siRNA molecule of claim 3 , wherein said siRNA molecule has no overhang regions.
Claim 12
Dependent Claim
56 words
A chemically synthesized double stranded siRNA molecule that directs cleavage of an mRNA via RNA interference, wherein: a. each strand of said double stranded siRNA molecule is between 19 and 30 nucleotides in length; and b. one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ. ID. NO. 461.
Claim 13
Dependent Claim
56 words
A chemically synthesized double stranded siRNA molecule that directs cleavage of an mRNA via RNA interference, wherein: a. each strand of said double stranded siRNA molecule is between 19 and 30 nucleotides in length; and b. one strand of said double stranded siRNA molecule comprises a sequence that is the complement of SEQ. ID. NO. 461.
Claim 14
Dependent Claim
24 words
The chemically synthesized double siRNA molecule of claim 11 , wherein each strand of said double stranded siRNA molecule is 19 nucleotides in length.
Claim 15
Dependent Claim
67 words
An siRNA molecule, wherein said siRNA molecule consists of: (a) a sense region, wherein said sense region consists of a sequence that is SEQ. ID. NO. 461, and an antisense region, wherein said antisense region consists of a sequence that is the complement of SEQ. ID. NO. 461; and (b) from zero to four overhang regions, wherein each overhang region is six or fewer nucleotides in length.
Claim 16
Dependent Claim
67 words
An siRNA molecule, wherein said siRNA molecule consists of: (a) a sense region, wherein said sense region consists of a sequence that is SEQ. ID. NO. 461, and an antisense region, wherein said antisense region consists of a sequence that is the complement of SEQ. ID. NO. 461; and (b) from zero to four overhang regions, wherein each overhang region is six or fewer nucleotides in length.
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| 3.5 Years (Year 4) | 2012-03-23 | 2012-09-22 | 2013-03-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2016-03-23 | 2016-09-22 | 2017-03-24 | $3760.00 | Window Closed / Lapsed |
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