US6885196B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biosensor
Assignee: PHC Holdings Corp
•
Filing: 2001-07-17
•
Granted: 2005-04-26
•
Expires: 2021-07-17
Abstract
Abstract A highly sensitive biosensor that uses a smaller amount of sample for measurement is described. The biosensor includes a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to the working electrode, and a reagent layer containing at least an oxidoreductase. The biosensor further includes a sample supply pathway formed between the first and second insulating base plates, where the working electrode, counter electrode and reagent layer are exposed to an inside of the sample supply pathway, and the distance between the working electrode and the counter electrode is 150 μm or less.
Inventors
- 👤 Yuko Taniike
- 👤 Shin Ikeda
- 👤 Shiro Nankai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:22
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/28; G01N27/30; G01N27/327; G01N27/3271; G01N27/3272; C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/006
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
111 words
A biosensor comprising a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to said working electrode, a reagent layer comprising at least an oxidoreductase, and a sample supply pathway formed between the first and second insulating base plates, wherein said working electrode, counter electrode and reagent layer are exposed to an inside of said sample supply pathway, and the distance between said working electrode and said counter electrode is 150 μm or less, and the area of said counter electrode exposed to the sample supply pathway is smaller than the area of said working electrode exposed to the sample supply pathway.
Claim 2
Dependent Claim
111 words
A biosensor comprising a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to said working electrode, a reagent layer comprising at least an oxidoreductase, and a sample supply pathway formed between the first and second insulating base plates, wherein said working electrode, counter electrode and reagent layer are exposed to an inside of said sample supply pathway, and the distance between said working electrode and said counter electrode is 150 μm or less, and the area of said counter electrode exposed to the sample supply pathway is smaller than the area of said working electrode exposed to the sample supply pathway.
Claim 3
Dependent Claim
59 words
The biosensor in accordance with claim 1 , wherein the area of said working electrode exposed to the sample supply pathway is 0.1 to 2.0 mm 2 , and the area of said counter electrode exposed to the sample supply pathway is in a range of not less than 0.05 mm 2 and less than 2.0 mm 2 .
Claim 4
Dependent Claim
24 words
The biosensor in accordance with claim 1 , wherein the distance between said working electrode and said counter electrode is 40 μm or more.
Claim 5
Dependent Claim
36 words
The biosensor in accordance with claim 1 , wherein a spacer member having a slit is interposed between the first and second insulating base plates such that said sample supply pathway is formed in said slit.
Claim 6
Dependent Claim
92 words
A biosensor comprising a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to said working electrode, a reagent layer comprising at least an oxidoreductase, and a sample supply pathway formed between the first and second insulating base plates, wherein said working electrode, counter electrode and reagent layer are exposed to said sample supply pathway, and the area of said counter electrode exposed to the sample supply pathway is less than the area of said working electrode exposed to the sample supply pathway.
Claim 7
Dependent Claim
92 words
A biosensor comprising a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to said working electrode, a reagent layer comprising at least an oxidoreductase, and a sample supply pathway formed between the first and second insulating base plates, wherein said working electrode, counter electrode and reagent layer are exposed to said sample supply pathway, and the area of said counter electrode exposed to the sample supply pathway is less than the area of said working electrode exposed to the sample supply pathway.
Claim 8
Dependent Claim
24 words
The biosensor in accordance with claim 5 , wherein the distance between said working electrode and said counter electrode is 150 μm or less.
References Cited (Backward Citations)
JPH03202764A
Cited
US5508171A
Cited
WO1991009139A1
Cited
US5437999A
Cited
EP0685737A1
Cited
JPH09159642A
Cited
EP0795601A2
Cited
JPH102874A
Cited
US6413410B1
Cited
WO1998035225A1
Cited
JPH11352093A
Cited
EP0964059A2
Cited
EP0969282A2
Cited
EP0969097A2
Cited
JP2000065777A
Cited
EP0984069A2
Cited
US6212417B1
Cited
JP2000258382A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2008-04-25 | 2008-10-25 | 2009-04-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2012-04-25 | 2012-10-25 | 2013-04-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2016-04-25 | 2016-10-25 | 2017-04-26 | $7700.00 | Window Closed / Lapsed |